Physical Insights into the Bio-preservation of Proteins by Glassy Solvents: Why is Glycerol better than Trehalose at low Temperatures?

Yıl 2020, Cilt: 8 Sayı: 3, 580 - 584, 30.09.2020

Taner Dirama

https://doi.org/10.21541/apjes.637368

Öz

Biopreservation has been a critical area of technical
and scientific research as it enables various forms biomolecular therapeutic
agents to find practical use in medicine. The mechanism at which biomimicry-inspired
solutions to stabilize biomolecules has been of a great scientific interest. We
have studied the behavior of lysozyme immersed in glycerol and trehalose, two
solvents frequently used in the bio-preservation of proteins, with the purpose
of identifying the microscopic origins of their very different dynamical
suppression capabilities. In agreement with experiments, we find that glycerol
is superior to trehalose at low temperatures, although the latter is deeper in
the glassy state, while trehalose is better at higher temperatures. We traced
the basis of this phenomenon to the different temperature dependencies of the
intermolecular hydrogen bonds between the model protein and the surrounding solvent.

Anahtar Kelimeler

structural dynamics, protein, solvent, biopreservation

Proteinlerin Camsı Solventlerle Biyolojik Korunması Üzerine Fiziksel Bilgiler: Gliserol, Düşük Sıcaklıklarda Neden Trehaloz'dan Daha İyidir?

Öz

Biyokoruma, çeşitli formlarda biyomoleküler terapötik
ajanların tıpta pratik kullanım bulmasını sağlayan kritik bir teknik ve
bilimsel araştırma alanı olmuştur. Temelini biyomimikriden alan biyomolekülleri
stabilize eden çözümlerin çalışma prensipleri bilimsel ilgi odağı olmuştur. Bu
çalışmada, lizozimin proteinlerin biyolojik olarak korunmasında sıkça
kullanılan iki çözücü olan gliserol ve trehaloz içerisindeki davranışlarını, bu
çözücülerin birbirinden farklı olan dinamik bastırma yeteneklerinin mikroskobik
kökenlerini belirlemek amacıyla inceledik. Gliserolün düşük sıcaklıklarda
trehaloza (trehalozun daha derin bir camsı halde olmasına rağmen) üstün
olduğunu, trehalozun ise yüksek sıcaklıklarda daha etkin olduğunu gözlemledik.
Bu sonucun kökeninin protein ve çözücü arasındaki moleküller arası hidrojen
bağlarının farklı sıcaklık bağımlılıklarına sahip oldukları sonucuna vardık.

Anahtar Kelimeler

yapısal dinamik, protein, çözücü, biyokoruma

Kaynakça

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