TNF-a TAYİNİ İÇİN REFLEKTOMETRİK İNTERFERANS SPEKTROSKOPİSİ TEMELLİ SENSÖR GELİŞTİRİLMESİ

Year 2010, Volume: 11 Issue: 2, 141 - 147, 22.10.2010

Sibel Emir Diltemiz , Ayça Atılır Özcan , Özlem Yalçınkaya

https://izlik.org/JA48AK25AS

Abstract

In this work, sensor systems based on reflectometric interference spectroscopy has been prepared for selectively recognition and binding of TNF-a biomolecules. For this purpose, carboxymethyldextran coated and reflectometric interference spectroscopy based sensor systems have been activated for binding of suitable orientated antibody, then anti-TNF-a antibody has been bounded on the surface and binding efficiency and binding constants of prepared sensors have been investigated

Keywords

-

TNF-α TAYİNİ İÇİN REFLEKTOMETRİK İNTERFERANS SPEKTROSKOPİSİ TEMELLİ SENSÖR GELİŞTİRİLMESİ

https://izlik.org/JA48AK25AS

Abstract

Bu çalışmada, TNF-α biyomolekülünü seçici olarak tanıyan ve bağlayabilen reflektometrik interferans spektroskopisi temelli sensör sistemi hazırlanmıştır. Bu amaçla, reflektometrik interferans spektroskopisi temelli ve karboksimetildekstran kaplı sensör sistemleri TNF-α antibadinin uygun yönlenimli bağlanabilmesi amacıyla aktive edilmiş, ardından uygun derişimdeki anti-TNF-α antibadi aktive yüzeylere bağlanmış ve hazırlanan sensörlerin TNF-α molekülünü bağlama etkinlikleri incelenerek, bağlanma sabitleri hesaplanmıştır.

Keywords

TNF-α , Reflektometrik interferans spektroskopisi , Biyosensör sistemleri

References

• Chen, G. ve Goeddel, D.V. (2002). TNF-R1 sig- naling: a beautiful pathway. Tularik Inc. 296, 1634-1635.
• Dana, L.D. ve Russell, C.J. (1991). In Vitro and In Vivo Induction of Tumor Necrosis Fac- tor Alpha by Borrelia burgdorferi. Infec- tion and Immunity 60(3) 1109-1113.
• Jacobs, C.B., Peairs, M.J. ve Venton, B.J. (2010). Review: Carbon nanotube based electrochemical sensors for biomolecules. Analytica Chimica Acta 662, (2, 10), 105- 127.
• Kittigul, L., Temprom, W., Sujirarat, D. ve Kit- tigul, C. (2000). A modified sandwich en- zyme-linked immunosorbent assay using biotin-streptavidin system (BS-ELISA) was developed to determine levels of tu- mor necrosis factor-alpha. Journal of Vi- rological Methods 90, 51-57.
• Mehdi, H., Razieh, Y., ve Fereidoun, A. (2001). Determination of Human Tumor Necrosis Factor a by a Highly Sensitive Enzyme Immunoassay. Biochemical and Biophysi- cal Research Communications 289, 295– 298.
• Old, L.J. (1985). Tumor necrosis factor (TNF). Science 230 630–632.
• Pearson, J.E., Gill, A. ve Vadgama, P. (2000). Analytical aspects of biosensors. Ann. Clin. Biochem. 37 119-145.
• Ping, T., Mien-Chie, H. and Jim, K. (1996). Human pro-Tumor Necrosis Factor is a Homotrimer. Biochemistry 35, 8216- 8225.
• Scheller, F.W., Wollenberger, U., Warsinke, A. and Lisdat, F. (2001). Research and de- velopment in biosensors. Current Opinion in Biotechnology 12 35–40.
• Sharma, S.K., Sehgal, N. ve Kumar, A. (2003). Biomolecules for development of biosen- sors and their applications. Current Ap- plied Physics 3(2-3), 307-316.
• Srinivasan, N. and Kilburn, J.D. (2004). Combi- natorial approaches to synthetic receptors. Current Opinion in Chemical Biology 8, 305–310.
• Tiina, S., Olli, C., Marja-Liisa, K., Anders, P., Ursula, T., Markku, K. ve Perttu, J.L. (2001). Evolution of Cerebral Tumor Ne- crosis Factor-a Production During Human Ischemic Stroke. Stroke 32, 1750-1758.
• Yagiuda, K., Hemmi, A. ve Ito, S. ve ark. (1996). Development of a conductivity- based immunosensor for sensitive detec- tion of methamphetamine (stimulant drug) in human urine. Biosens Bioelectron 11, 703-707.
• Wanachiwanawin, W., Wiener, E., Siripan- yaphinyo, U., Chinprasertsuk, S., Mawas, F., Fucharoen, S., Wickramasinghe, S.N., Pootrakul, P. ve Visudhiphan, S. (1999). Serum Levels of Tumor Necrosis Factor- alpha, Interleukin-1, and Interferon- gamma in beta-Thalassemia/HbE and Their Clinical Significance. Journal of In- terferon & Cytokine Research 19, 105- 111.
• Wang, J., Nielsen, P., Jiang, M., Cai, X., Fer- nandez, J.R., Grant, D., Ozsoz, M., Begle- iter, A. ve Mowat, M. (1997). Mismatch sensitive hybridization detection by PNA immobilized on QCM. Anal. Chem. 69, 5200–5202.