Use of PCR for detection of Burkholderia mallei in Türkiye

Abstract

Within the scope of the "National Ruam Eradication Project" carried out between 2000-2001, Glanders was eradicated in our country. Unfortunately, 81 horses were culled in Türkiye in December 2019, following the detection of Epidemic in horses in Büyükada. In 2019, Glanders were reported in horses in Uşak and Bolu. No human cases have been reported. Türkiye is at risk for Glanders because of its geography. Therefore, reliable and fastly detection of Burkholderia mallei by PCR in a short time will prevent the distribution of unwanted infections that may occur in the future throughout the country. In this study; Burkholderia mallei strains isolated from horses were verified and optimized by PCR. The use of PCR for the detection of Burkholderia mallei was performed for the first time in our country. It has been concluded that the PCR as a diagnostic method with high reliability and sensitivity safely used together with diagnosis of Glanders.

Keywords

• burkholderia mallei
• CBRN
• glanders
• PCR

References

1. Cárdenas NC, Galvis JO, Farinati AA, et al (2019): Burkholderia mallei: The dynamics of networks and disease transmission. Transbound Emerg Dis, 66, 715-728.
2. Elschner MC, Klaus CU, Liebler‐Tenorio E, et al (2009): Burkholderia mallei infection in a horse imported from Brazil. Equine Vet Educ, 21, 147-150.
3. Falcao MV, Laroucau K, Vorimore F, et al (2022). Molecular characterization of Burkholderia mallei strains isolated from horses in Brazil (2014–2017). Infection Genetics and Evolution, 99, 105250.
4. Fonseca Júnior AA, Pinto CA, Alencar CA, et al (2021): Validation of three qPCR for the detection of Burkholderia mallei in equine tissue samples. Arch Microbiol, 203, 3965–3971.
5. Gee JE, Sacchi CT, Glass MB, et al (2003): Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol, 41, 4647-4654.
6. Gilling DH, Luna VA, Pfugradt C (2014): The identification and differentiation between Burkholderia mallei and Burkholderia pseudomallei using one gene pyrosequencing. Int Sch Res Notices, 2, 109583.
7. Girault G, Wattiau P, Saqib M, et al (2018): High-resolution melting PCR analysis for rapid genotyping of Burkholderia mallei. Infect Genet Evol, 63, 1–4.
8. Ghori MT, Khan MS, Khan JA, et al (2018): Molecular detection of Burkholderia mallei in nasal swabs from draught horses with signs of respiratory tract infection. J Anim Plant Sci, 28, 1717-1724.

9. Godlewsky S (1972): Türkiye'de Veteriner Hekimlik (19'uncu Yüzyıl Ortalarında). Çev. Nihal Erk. Ankara Univ Vet Fak Yayınları, 281.
10. Hornstra H, Pearson T, Georgia S, et al (2009): Molecular epidemiology of glanders, Pakistan. Emerg Infect Dis, 15, 2036–2039.
11. Khan I, Wieler LH, Melzer F, et al (2013): Glanders in animals: A review on epidemiology, clinical presentation, diagnosis and countermeasures. Transbound Emerg Dis, 60, 204–221.
12. Laroucau K, Aaziz R, Vorimore F, et al (2021): A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders. Transbound Emerg Dis, 68, 960-963.
13. Merwyn S, Kumar S, Agarwal GS, et al (2010): Evaluation of PCR, DNA hybridization and immunomagnetic separation-PCR for detection of burkholderia mallei in artificially inoculated environmental samples. Ind J Mic, 50, 172-178.
14. Najafpour R, Mosavari N, Tadayon K, et al (2015): Optimization of variable number tandem repeat (VNTR) analysis in the classical PCR machines for typing of Burkholderia mallei. J Microbiol, 8, 190-199.
15. Neubauer H, Sprague LD, Zacharia R, et al (2005): Serodiagnosis of Burkholderia mallei infections in horses: state‐of‐the‐art and perspectives. J Vet Med B, 52, 201-205.
16. OIE (2018): Chapter 2.5.11: Glanders and melioidosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Available at: http://www.oie.int/fileadmin/Home/ eng/Health_standards/tahm/2.05.11_GLANDERS.pdf (Accessed January 25, 2021).
17. OIE (2020): Chapter 3.5.11: Glanders and melioidosis. In: Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Available at: https://www.oie.int/fileadmin/ Home/eng/Health_standards/tahm/3.05.11_GLANDERS.pdf. (Accessed May 21, 2021).
18. Osmanağaoğlu Ş, Melikoğlu B (2009): Türkiye’de Ruam hastalığı eradikasyon çalışmalarına tarihsel bir bakış açısı. Kafkas Univ Vet Fak Derg, 15, 331-337.
19. Öztürk R, Başağaç RT (2002): Veteriner hekimliği tarihinde iz bırakanlar Hüdai-Ahmet-Kemal Cemil. Türk Vet Hek Birl Derg, 2, 54-56.
20. Pakdemirli A, Dülger D (2021): Tarihsel bir biyolojik ajan ve KBRN açısından önemi: Ruam (Glanders) “Burkholderia mallei”. Etlik Vet Mikrobiyol Derg, 32, 1-7.
21. Saxena A, Pal V, Tripathi NK, et al (2019): Development of a rapid and sensitive recombinase polymerase amplification-lateral flow assay for detection of Burkholderia mallei. Transbound Emerg Dis, 66, 1016-1022.
22. Shanmugasundaram K, Singha H, Saini S, et al (2022): 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013–2019). Current Microbiology, 79, 1-13.
23. Singha H, Vorimore F, Saini S, et al (2021): Molecular epidemiology of Burkholderia mallei isolates from India (2015–2016): New SNP markers for strain tracing. Infect Genet Evol, 95, 105059.
24. Scholz HC, Joseph M, Tomasso H, et al (2006): Detection of the reemerging agent Burjholderia mallei in a recent outbreak of glanders in the United Arab Emirates by a newly flip- based polymerase chain reaction assay. Diagn Microbiol Infect Dis, 54, 241-247.
25. Torba TA (2020): Burkholderia mallei: Ruam Hastalığı/Burkholderia mallei: Glanders. ESTÜDAM Halk Sağl Derg, 5, 353-361.