Metformin ve Farklı Doğal Ürün Kombinasyonları ile Tedavi Edilen Diyabetik Sıçanların İdrar Örneklerinin Değerlendirilmesi

Abstract

Diyabet tüm dünyada yaygın bir hastalık olup diyabetik hayvanlar için glukozüri ve diyabetik nefropatiyi kontrol etmek amacıyla dipstick ile idrar analizi önemli bir ölçümdür. Bu çalışmanın amacı, diyabette hipoglisemik ilaç kullanımında ve ilaç ve doğal ürün kombinasyonunda glukozüri, ketonüri ve proteinüri değişikliklerini izlemektir. Tip 2 diyabet streptozotosin (65 mg/kg, i.p) ve nikotinamid (110 mg/kg, i.p) ile oluşturulmuştur. İlaç ve doğal ürünler 84 gün boyunca oral yolla verilmiş ve deney sonunda idrar örnekleri toplanmıştır. İdrar tahlili (glikoz, keton ve protein) dipstick ile yapılmıştır. Sonuçlar 0-3 arasında skorlanarak Kruskal Wallis analizi uygulanmıştır. Tedavi edilmemiş diyabetik grup (DM) ile diğer gruplar arasında glukoz açısından anlamlı bir fark bulunmuş, ancak keton ve protein analizleri istatistiksel olarak anlamlı bir fark göstermemiştir. Sonuçlar, ilaç ve ilaç+doğal ürün kombinasyonunun diyabette idrar glukoz atılımını azalttığını göstermiştir. Sonuç olarak, metformin veya/ve doğal ürün kombinasyonlarının kullanımı idrarda glukoz çıkışını azaltmıştır. İdrarda glukoz testinin dipstick gibi kolay ve ucuz bir izleme yöntemi ile diyabetin geç komplikasyonları (nefropati) ortaya konabilir ve ilaç ve doğal ürün kombinasyonlarının etkisi izlenebilir.

Keywords

• diyabet
• ilaç
• doğal ürün
• idrar analizi

Supporting Institution

Bu çalışma Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü ve Türkiye Sağlık Enstitüleri Başkanlığı tarafından onaylanan projelerden alınan destek ile yapılmıştır

Project Number

21L0239018 (Bilimsel Araştırma Projeleri Koordinatörlüğü Ankara Üniversitesi) ve 16614 (Türkiye Sağlık Enstitüleri Başkanlığı)

Ethical Statement

Bu çalışma, Ankara Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu tarafından onaylanmıştır (Karar numarası: 2021-5-29).

Thanks

Bu çalışma Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 21L0239018 proje numarası ve Türkiye Sağlık Enstitüleri Başkanlığı tarafından 16614 proje numarası ile desteklenmiştir.

Evaluation of urine samples of diabetic rats treated with metformin and different natural product combinations

Abstract

Diabetes is highly prevalent worldwide, and urine analyses with dipstick methods are important tools to monitor glucosuria and nephron status in diabetic animals. The aim of this study is to follow glucosuria, ketonuria, and proteinuria changes in hypoglycemic drug use in diabetes and drug and natural product combinations. Male wistar albino rats were used in the study and type 2 diabetes was induced with streptozotocin (65 mg/kg, i.p.) and nicotinamide (110 mg/kg, i.p.). The drug and natural products were administered orally for a period of 84 days (healthy control, diabetic, diabetic+metformin, diabetic+metformin+cherry laurel, diabetic+metformin+rutin and diabetic+metformin+alpha lipoic acid groups), and the urine samples were collected at the end of the experiment. The urinalysis (glucose, ketone, and protein) was done with a dipstick. The results were scored between 0 and 3, and Kruskal-Wallis analysis was applied. There was a significant difference between the untreated diabetic group (DM) and the remaining groups in glucose, but ketone and protein analysis did not show any statistically significant differences. The results showed that drug and drug+natural product combination reduced urinary glucose excretion in diabetes. In conclusion, the use of metformin and/or natural product combinations decreased the glucose output in urine. With an easy and cheap monitoring method such as a dipstick, the metabolic state can be revealed. And the effect of drug and natural product combinations can be monitored.

Keywords

• Diabetes
• Metformin
• Natural Products
• Urinalysis

Supporting Institution

This study was carried out with the support received from the projects approved by Ankara University Scientific Research Projects Coordination Office and Turkish Health Institutes.

Project Number

21L0239018 (Bilimsel Araştırma Projeleri Koordinatörlüğü Ankara Üniversitesi) ve 16614 (Türkiye Sağlık Enstitüleri Başkanlığı)

Ethical Statement

This study was carried out after the animal experiment was approved by Ankara University Local Ethics Committee of Animal Experiments (Decision number: 2021-5-29).

Thanks

This study was done with the support of Scientific Research Projects Coordination Office of Ankara University with the 21L0239018 project number and The Health Institutes of Türkiye with the 16614 project number.

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