Effects of dietary propolis supplementation on fattening performance, carcass traits and meat quality in New Zealand White rabbits

Abstract

The aim of the study was to determine the effects of dietary propolis extract supplementation at different doses on the fattening performance, slaughter characteristics, meat quality, and fatty acid composition of New Zealand White rabbits. A total of 72 weaned, mixed-sex New Zealand White rabbits, aged one month, were used in the study. The rabbits were randomly assigned to three groups: one control (C) and two experimental groups (P1 and P2) (n = 24 per group). The control group was fed a standard ration, while the P1 and P2 groups received the same ration supplemented with 1000 mg/kg and 5000 mg/kg of propolis extract, respectively. The fattening period lasted for 8 weeks. Daily feed intake and feed conversion ratio were significantly higher in the P2 group (P˂0.001 and P˂0.01, respectively). The dressing percentage was highest in the C group (P˂0.05). Kidney and spleen percentages were lower in C group than experimental groups (P˂0.001 and P˂0.05, respectively). The pH and color parameters of the meat were within optimal ranges. The P2 group had the highest values for expressed juice loss and cooking loss (P˂0.001 and P˂0.05, respectively). There were no significant differences among groups for drip loss and calculated fatty acid ratios. In conclusion, dietary supplementation of propolis extract increased feed intake, carcass yield, spleen, and kidney percentages and cooking loss; while it decreased expressed juice, and had no significant effect on fatty acid composition except for a few saturated and unsaturated fatty acids.

Keywords

• Fattening performance
• Fatty acid composition
• Meat quality
• Propolis
• Rabbit

Supporting Institution

Balıkesir Üniversitesi

Project Number

Balıkesir Üniversitesi BAP 2018/063

References

1. Abdel-Khalek AM (2013): Supplemental antioxidants in rabbit nutrition: A review. Livest Sci, 158, 95-105.
2. Abdel-Wareth AAA, Taha EMM, Südekum KH, et al (2018): Thyme oil inclusion levels in a rabbit ration: Evaluation of productive performance, carcass criteria and meat quality under hot environmental conditions. Anim Nutr, 4, 410-416.
3. Agradi S, Sulce M, Menchetti L, et al (2023): Dietary supplementation with n-3 polyunsaturated fatty acids: Effects on reproductive and productive performance and meat quality in rabbit breeding. Anim Nutr, 14, 70-78.
4. Akçapınar A, Özbeyaz C (2021): Animal Husbandry (Basic Knowledge). Medisan Publishing, Ankara.
5. Al-Homidan I, Fathi M, Abdelsalam M, et al (2022): Effect of propolis supplementation and breed on growth performance, immunity, blood parameters and cecal microbiota in growing rabbits. Anim Biosci, 35, 1606-1615.
6. Assan N (2017): Carcass traits and meat quality properties as affected by nutritional programs and feeding patterns in a rabbit enterprise. Sci J Anim Sci, 6, 459-470.
7. Attia YA, Bovera F, Abd-Elhamid A, et al (2019): Evaluation of the carryover effect of antibiotic, bee pollen and propolis on growth performance, carcass traits and splenic and hepatic histology of growing rabbits. J Anim Physiol Anim Nutr, 103, 947-958.
8. Attia YA, El-Hanoun AM, Bovera F, et al (2014): Growth performance, carcass quality, biochemical and haematological traits and immune response of growing rabbits as affected by different growth promoters. J Anim Physiol Anim Nutr, 98, 128-139.

9. Beriain MJ, Horcada A, Purroy A, et al (2000): Characteristics of Lacha and Rasa Aragonesa lambs slaughtered at three live weights. J Anim Sci, 78, 3070-3077.
10. Bhatt RS, Sarkar S, Sharma SR, et al (2023): Use of moringa oleifera leaves (sole or combined with concentrate) in rabbit feeding: Effects on performance, carcass characteristics and meat quality attributes. Meat Sci, 198, 109108.
11. Blasco A, Piles M (1990): Muscular pH of the rabbit. Ann Zootech, 39, 133-136.
12. Blasco A, Quhayoun J (1993): Harmonization of criteria and terminology in rabbit meat research. Revised proposal. World Rabbit Sci, 4, 93-99.
13. Blight E, Dyer W (1959): A rapid method of total lipid extraction and purification. Can J Biochem Physiol, 37, 911-917.
14. Blonska M, Bronikowska J, Pietsz G, et al (2004): Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages. J Ethnopharmacol, 91, 25-30.
15. Celia C, Cullere M, Gerencsér Z, et al (2016): Effect of pre- and post-weaning dietary supplementation with Digestarom herbal formulation on rabbit carcass traits and meat quality. Meat Sci, 118, 89-95.
16. Cullere M, Dalle Zotte A (2018): Rabbit meat production and consumption : State of knowledge and future perspectives. Meat Sci, 143, 137-146.
17. Da Costa MFB, Libório AB, Teles F, et al (2015): Red propolis ameliorates ischemic-reperfusion acute kidney injury. Phytomedicine, 22, 787-795.
18. Da Silva JA, Ítavo CCBF, Ítavo LCV, et al (2019): Dietary addition of crude form or ethanol extract of brown propolis as nutritional additive on behaviour, productive performance and carcass traits of lambs in feedlot. J Anim Feed Sci, 28, 31-40.
19. Dalle Zotte A (2002): Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livest Prod Sci, 75, 11-32.
20. Dalle Zotte A, Szendrő Z (2011): The role of rabbit meat as functional food. Meat Sci, 88, 319-331.
21. Dalle Zotte A, Cullere M, Tasoniero G, et al (2018): Supplementing growing rabbit diets with chestnut hydrolyzable tannins: Effect on meat quality and oxidative status, nutrient digestibilities, and content of tannin metabolites. Meat Sci, 146, 101-108.
22. Demir MT, Ünal N, Onbaşılar EE (2025): The effects of LED lights in different colors on fattening performance, litter characteristics, meat properties, and some welfare parameters in broilers. Ankara Univ Vet Fak Derg, 72, 77-82.
23. Dias DMB, de Oliveira MC, da Silva DM, et al (2013): Suplementação com pólen apícola em dietas para coelhas e coelhos em crescimento. Acta Sci - Anim Sci, 35, 425-430.
24. Dutra DR, Villegas-Cayllahua EA, Baptista GG, et al (2024): Characterization of post-mortem Ph evolution and rigor mortis process in Botucatu rabbit carcasses of different categories. Animals, 14, 2502.
25. El-Sabrout K, Khalifah A, Ciani F (2023): Current applications and trends in rabbit nutraceuticals. Agriculture, 13, 1-18.
26. Forrester-Anderson IT, McNitt J, Way R, et al (2006): Fatty acid content of pasture-reared fryer rabbit meat. J Food Compost Anal, 19, 715-719.
27. Gabr S, Younan G, Hamad M, et al (2016): Effect of some natural antioxidants on growth performance, blood parameters and carcass traits of growing rabbits under Egyptian summer condition. J Anim Poultry Prod, 7, 457-466.
28. Galeano-Díaz JP, Sánchez-Torres JE, Domínguez-Vara IA, et al (2023): Effects on growth performance parameters, carcass traits, meat nutrimental quality and intramuscular fatty acid profile of rabbits fed with diets with avocado waste (Persea americana Mill). Agriculture, 13, 549.
29. Grau R, Hamm R (1957): Über das wasserbindungsvermogen des saugetiermuskels. II. Mitteilung. Z Lebensm Unters Forsch, 105, 440–446.
30. Hashem NM, Abd El-Hady AM, Hassan OA (2017): Inclusion of phytogenic feed additives comparable to vitamin E in diet of growing rabbits: Effects on metabolism and growth. Annals Agric Sci, 62, 161-167.
31. Hernández P, Zotte AD (2010): Influence of Diet on Rabbit Meat Quality. 163-178. In: C de Blas, J Wiseman (Ed) Nutrition of the Rabbit. CAB International: Cambridge USA.
32. Honikel KO (1998): Reference methods for the assessment of physical characteristics of meat. Meat Sci, 49, 447-457.
33. Hughes JM, Oiseth SK, Purslow PP, et al (2014): A structural approach to understanding the interactions between colour, water-holding capacity and tenderness. Meat Sci, 98, 520-532.
34. Itavo CCBF, Itavo LCV, Esteevs CAT, et al (2019): Influence of solid residue from alcoholic extraction of brown propolis on intake, digestibility, performance, carcass and meat characteristics of lambs in feedlot. J Anim Feed Sci, 28, 149-158.
35. Kaya M, Çetinkaya MA (2025): Radiographic cardiac indices for healthy New Zealand White rabbits: A reference interval study based on echocardiography. Ankara Univ Vet Fak Derg, 72, 105-112.
36. Kerth CR (2013): The Science of Meat Quality. Wiley-Blackwell, USA.
37. Kołodziej-Skalska A, Rybarczyk A, Matysiak B, et al (2011): Effect of dietary plant extracts mixture on pork meat quality. Acta Agriculturae Scand Section A, 61, 80-85.
38. Koné AP, Desjardins Y, Gosselin A, et al (2019): Plant extracts and essential oil product as feed additives to control rabbit meat microbial quality. Meat Sci, 150, 111-121.
39. Mapiye C, Chimonyo M, Dzama K, et al (2011): Fatty acid composition of beef from Nguni steers supplemented with Acacia karroo leaf-meal. J Food Compost Anal, 24, 523-528.
40. Meineri G, Cornale P, Tassone S, et al (2009): Effects of chia (Salvia hispanica L.) seed supplementation on rabbit meat quality, oxidative stability and sensory traits. Ital J Anim Sci, 9, 45-49.
41. North MK, Dalle Zotte A, Hoffman L (2019): The use of dietary flavonoids in meat production: A review. Anim Feed Sci Technol, 257, 114291.
42. Onbaşılar EE, Onbaşılar İ (2007): Effect of cage density and sex on growth, food utilization and some stress parameters of young rabbits. Scand J Lab Anim Sci, 34, 1-7.
43. Onbaşılar İ, Yalçın S (2008): The effects of dietary supplementation of probiotic and anticoccidial additives on performance and blood parameters in growing rabbits. Revue Med Vet, 159, 570-574.
44. Peker A, Orkan Ş, Aral Y, et al (2024): A Comprehensive outlook on cultured meat and conventional meat production. Ankara Univ Vet Fak Derg, 71, 511-522.
45. Piza PC, Moreira BL, Silva NCD, et al (2021): Effect of crude propolis on the performance and feed digestibility of new zealand white rabbits. Acta Sci Anim Sci, 43, 1-6.
46. Prakatur I, Miškulin I, Senčić D, et al (2020) :The influence of propolis and bee pollen on chicken meat quality. Vet Arhiv, 90, 617-625.
47. Rossi R, Vizzarri F, Chiapparini S, et al (2020): Effects of dietary levels of brown seaweeds and plant polyphenols on growth and meat quality parameters in growing rabbit. Meat Sci, 161, 107987.
48. Sadarman Irawan A, Hidayat C, Elfawati Sholikin MM, et al (2021): Propolis supplementation on broiler chicken performances, nutrient digestibility, and carcass characteristics: A Meta-analysis. Trop Anim Sci J, 44, 425-433.
49. Saeed M, Arain M, Kamboh A, et al (2017): Raw propolis as a promising feed additive in poultry nutrition: Trends and advances. J Anim Health Prod, 5, 132-142.
50. Santos-Silva J, Bessa RJB, Santos-Silva F (2002): Effect of genotype, feeding system and slaughter weight on the quality of light lambs II. Fatty acid composition of meat. Livest Prod Sci, 77, 187-194.
51. Shreif E, El-Saadany A (2016): The effect of supplementing diet with propolis on Bandarah laying hens’ performance. Egypt Poult Sci, 36, 481-499.
52. Siddiqui SA, Gerini F, Ikram A, et al (2023): Rabbit meat—production, consumption and consumers’ attitudes and behavior. Sustainability, 15, 2008.
53. Sierra-Galicia MI, Rodríguez-de Lara R, Orzuna-Orzuna JF, et al (2022): Supplying bee pollen and propolis to growing rabbits: Effects on growth performance, blood metabolites, and meat quality. Life, 12, 1-15.
54. Sierra-Galicia MI, Rodríguez-de Lara R, Orzuna-Orzuna JF, et al (2023): Effects of supplementation with bee pollen and propolis on growth performance and serum metabolites of rabbits: A Meta-analysis. Animals, 13, 1-17.
55. Silveira MAD, Capcha JMC, Sanches TR, et al (2021): Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis. Sci Rep, 11, 1-11.
56. Tatlı Seven P, Seven I, Yılmaz M, et al (2008): The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim Feed Sci Technol, 146, 137-148.
57. Tejeda JF, Peña RE, Andrés AI (2008): Effect of live weight and sex on physico-chemical and sensorial characteristics of Merino lamb meat. Meat Sci, 80, 1061-1067.
58. Ulbricht T, Southage D (1991): Coronary heart disease: Seven dietary factors. Lancet, 338, 985–992.
59. Valero MV, Zeoula LM, Peres De Moura LP, et al (2015): Propolis extract in the diet of crossbred (1/2 Angus vs. 1/2 Nellore) bulls finished in feedlot: Animal performance, feed efficiency and carcass characteristics. Semina: Cien Agrar, 36, 1067-1078.
60. Waly A, Abo El-Azayem E, Younan G, et al (2021): Effect of propolis supplementation on growth performance, nutrients digestibility, carcass characteristics and meat quality of growing New Zealand rabbits. Egypt J Nutr Feeds, 24, 65-73.
61. Wołoszyn J, Haraf G, Okruszek A, et al (2020): Fatty acid profiles and health lipid indices in the breast muscles of local Polish goose varieties. Poult Sci, 99, 1216-1224.
62. Wood JD, Enser M (1997): Factors influencing fatty acids in meat and the role of antioxidants in improving meat quality. Br J Nutr, 78, 49-60.
63. Wood JD, Richardson RI, Nute GR, et al (2003): Effects of fatty acids on meat quality: a review. Meat Sci, 66, 21-32.
64. Yalçın S, Onbaşılar EE, Onbaşılar İ (2006): Effect of sex on carcass and meat characteristics of New Zealand White rabbits aged 11 weeks. Asian-Aust J Anim Sci, 19, 1212-1216.
65. Yalçın S, Tuncer İ, Yalçın S, et al (2003): The use of different levels of common vetch seed (Vicia sativa L.) in diets for fattening rabbits. Livest Prod Sci, 84, 93-97.