Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye

Öykü Barılı; Şinasi Umur

doi:10.33988/auvfd.1703476

Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye

Abstract

Tetraserialis tscherbakovi (Petrov and Chertkova, 1960) is a rare monostomic trematode belonging to the family Notocotylidae. This study aimed to provide detailed information on Tetraserialis tscherbakovi. In this study, the digestive systems of 25 rodents captured in the Sarıkamış district of Kars Province were examined, and 19 trematodes morphologically consistent with Tetraserialis tscherbakovi were detected in a single Microtus mystacinus individual. Light microscopy images of the parasites were obtained, along with scanning electron microscopy (SEM) images, which are presented for the first time worldwide. SEM examination revealed, in detail, a previously undescribed cap-like helical structure of the eggs. The sequencing of the ITS1, 5.8S rRNA, and ITS2 gene regions was conducted; the obtained isolate was deposited in GenBank (PP812223). BLAST analysis showed the highest similarity to Notocotylus malhamensis (97.54%) and Notocotylus sp. (96.89%). Since these values are below the generally accepted threshold for definitive species-level molecular identification, the molecular data were considered preliminary and supported by morphological findings. Since the analysis was based only on the ITS1–5.8S–ITS2 region and showed similarity values slightly below the accepted species-level threshold, the molecular results should be interpreted with caution until additional markers (e.g., COI, 28S) are studied. This study reports the first morphologically identified case of Tetraserialis tscherbakovi from M. mystacinus in Türkiye and provides preliminary data on the phylogenetic position of the species.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Parasitology

Journal Section

Research Article

Authors

Öykü Barılı ^*
0000-0001-7692-5171
Türkiye

Şinasi Umur
0000-0001-9766-2817
Türkiye

Early Pub Date

November 25, 2025

Publication Date

January 1, 2026

Submission Date

May 21, 2025

Acceptance Date

October 7, 2025

Published in Issue

Year 2026 Volume: 73 Number: 1

DOI

https://doi.org/10.33988/auvfd.1703476

IZ

https://izlik.org/JA54JU37UW

Cite

RIS / Bibtex

APA

Barılı, Ö., & Umur, Ş. (2026). Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 73(1), 33-39. https://doi.org/10.33988/auvfd.1703476

AMA

1.Barılı Ö, Umur Ş. Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye. Ankara Univ Vet Fak Derg. 2026;73(1):33-39. doi:10.33988/auvfd.1703476

Chicago

Barılı, Öykü, and Şinasi Umur. 2026. “Morphological Confirmation and Preliminary Molecular Data on Tetraserialis Tscherbakovi from Microtus Mystacinus in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 (1): 33-39. https://doi.org/10.33988/auvfd.1703476.

EndNote

Barılı Ö, Umur Ş (January 1, 2026) Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 1 33–39.

IEEE

[1]Ö. Barılı and Ş. Umur, “Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye”, Ankara Univ Vet Fak Derg, vol. 73, no. 1, pp. 33–39, Jan. 2026, doi: 10.33988/auvfd.1703476.

ISNAD

Barılı, Öykü - Umur, Şinasi. “Morphological Confirmation and Preliminary Molecular Data on Tetraserialis Tscherbakovi from Microtus Mystacinus in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73/1 (January 1, 2026): 33-39. https://doi.org/10.33988/auvfd.1703476.

JAMA

1.Barılı Ö, Umur Ş. Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye. Ankara Univ Vet Fak Derg. 2026;73:33–39.

MLA

Barılı, Öykü, and Şinasi Umur. “Morphological Confirmation and Preliminary Molecular Data on Tetraserialis Tscherbakovi from Microtus Mystacinus in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 73, no. 1, Jan. 2026, pp. 33-39, doi:10.33988/auvfd.1703476.

Vancouver

1.Öykü Barılı, Şinasi Umur. Morphological confirmation and preliminary molecular data on Tetraserialis tscherbakovi from Microtus mystacinus in Türkiye. Ankara Univ Vet Fak Derg. 2026 Jan. 1;73(1):33-9. doi:10.33988/auvfd.1703476