In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential

Izzet Burcin Saticioglu; Nihed Ajmi; Esra Demirbaş; Cansu Aktaş; Ertan Emek Onuk

doi:10.33988/auvfd.1748170

In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential

Abstract

Edwardsiella tarda is a facultatively anaerobic, Gram-negative bacterium that is widely distributed in aquatic environments. With the increasing interactions between aquatic animals and humans, E. tarda has become an important pathogen with significant implications for global public health. In this study, we characterized strain ET-1, isolated from an aquarium housing Pangasius pangasius, to evaluate its ecological presence, antimicrobial resistance, and pathogenic potential using a combination of phenotypic and genome-based approaches. Biochemical characteristics were determined using the API 20E system. Antimicrobial susceptibility was evaluated using MIC testing to determine resistance to clinically relevant antibiotics. Whole-genome sequencing using Oxford Nanopore Technologies revealed a genome size of 3.76 Mb with a GC content of 57.12%. Genome-based identification using digital DNA–DNA hybridization (dDDH, 86.6%) confirmed the classification as E. tarda. Several virulence-associated genes (flgG – flagellar basal body-rod protein; etfC – electron transfer flavoprotein subunit C) and antimicrobial resistance determinants (qnrS1 – quinolone resistance protein S1; efflux pump genes such as kpnH and kpnF) were identified. Pathogenic potential analysis via PathogenFinder2 indicated a high likelihood of human pathogenicity. Additionally, 16S rRNA-based profiling revealed the widespread environmental and host-associated presence of ET-1-like sequences. These results underscore the zoonotic and environmental significance of E. tarda ET-1 and support its relevance for public health surveillance within the One Health framework.

Keywords

Project Number

THIZ-2024-2071

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Details

Primary Language

English

Subjects

Veterinary Microbiology

Journal Section

Research Article

Authors

Izzet Burcin Saticioglu
0000-0002-2721-3204
Türkiye

Nihed Ajmi
0000-0002-5368-4819
Türkiye

Esra Demirbaş
0000-0002-3708-0804
Türkiye

Cansu Aktaş
0000-0003-3869-870X
Türkiye

Ertan Emek Onuk ^*
0000-0001-7643-046X
Türkiye

Early Pub Date

December 2, 2025

Publication Date

April 1, 2026

Submission Date

July 22, 2025

Acceptance Date

November 19, 2025

Published in Issue

Year 2026 Volume: 73 Number: 2

DOI

https://doi.org/10.33988/auvfd.1748170

IZ

https://izlik.org/JA25SB92FZ

Cite

RIS / Bibtex

APA

Saticioglu, I. B., Ajmi, N., Demirbaş, E., Aktaş, C., & Onuk, E. E. (2026). In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 73(2), 121-126. https://doi.org/10.33988/auvfd.1748170

AMA

1.Saticioglu IB, Ajmi N, Demirbaş E, Aktaş C, Onuk EE. In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential. Ankara Univ Vet Fak Derg. 2026;73(2):121-126. doi:10.33988/auvfd.1748170

Chicago

Saticioglu, Izzet Burcin, Nihed Ajmi, Esra Demirbaş, Cansu Aktaş, and Ertan Emek Onuk. 2026. “In Silico Characterization of Edwardsiella Tarda Strain ET-1 Isolated from Aquarium Water Reveals Zoonotic and Pathogenic Potential”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 (2): 121-26. https://doi.org/10.33988/auvfd.1748170.

EndNote

Saticioglu IB, Ajmi N, Demirbaş E, Aktaş C, Onuk EE (April 1, 2026) In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 2 121–126.

IEEE

[1]I. B. Saticioglu, N. Ajmi, E. Demirbaş, C. Aktaş, and E. E. Onuk, “In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential”, Ankara Univ Vet Fak Derg, vol. 73, no. 2, pp. 121–126, Apr. 2026, doi: 10.33988/auvfd.1748170.

ISNAD

Saticioglu, Izzet Burcin - Ajmi, Nihed - Demirbaş, Esra - Aktaş, Cansu - Onuk, Ertan Emek. “In Silico Characterization of Edwardsiella Tarda Strain ET-1 Isolated from Aquarium Water Reveals Zoonotic and Pathogenic Potential”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73/2 (April 1, 2026): 121-126. https://doi.org/10.33988/auvfd.1748170.

JAMA

1.Saticioglu IB, Ajmi N, Demirbaş E, Aktaş C, Onuk EE. In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential. Ankara Univ Vet Fak Derg. 2026;73:121–126.

MLA

Saticioglu, Izzet Burcin, et al. “In Silico Characterization of Edwardsiella Tarda Strain ET-1 Isolated from Aquarium Water Reveals Zoonotic and Pathogenic Potential”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 73, no. 2, Apr. 2026, pp. 121-6, doi:10.33988/auvfd.1748170.

Vancouver

1.Izzet Burcin Saticioglu, Nihed Ajmi, Esra Demirbaş, Cansu Aktaş, Ertan Emek Onuk. In silico characterization of Edwardsiella tarda strain ET-1 isolated from aquarium water reveals zoonotic and pathogenic potential. Ankara Univ Vet Fak Derg. 2026 Apr. 1;73(2):121-6. doi:10.33988/auvfd.1748170