First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye

Seyyide Sarıçam İnce; Mehmet Akan

doi:10.33988/auvfd.1774791

First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic and versatile pathogen that causes infections in both humans and animals. It is one of the ESKAPE pathogens, characterized by the ability to rapidly acquire resistance to antimicrobials. In recent years, multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR) P. aeruginosa have been reported worldwide in humans and chickens. In poultry, P. aeruginosa is frequently isolated and spreads easily due to its multiple transmission routes and its ability to infect birds of all ages. As a zoonotic pathogen, it poses a risk to humans via the consumption of contaminated poultry products. This study aimed to isolate P. aeruginosa from a layer chicken flock in Türkiye and investigate its antimicrobial resistance (AMR) and biofilm formation ability. Isolation and identification were performed by conventional bacteriological analysis and sequence analysis of the 16S rRNA gene. Following phenotypic AMR testing, biofilm formation was quantitatively analyzed, and eleven AMR genes were screened by PCR. The recovered P. aeruginosa isolate exhibited resistance to aztreonam, colistin, and meropenem, thereby meeting the criteria for MDR. It also tested positive for blaOXA and mcr-2 resistance genes and was defined as a weak biofilm producer. To the best of our knowledge, this is the first report of blaOXA and mcr-2 genes co-existing in an MDR P. aeruginosa isolate recovered from a layer chicken flock in Türkiye. These findings highlight the potential risk posed by MDR and biofilm forming P. aeruginosa in poultry environments to both human and animal health.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Bacteriology

Journal Section

Case Report

Authors

Seyyide Sarıçam İnce ^*
0000-0002-2386-6857
Türkiye

Mehmet Akan
0000-0002-7342-1450
Türkiye

Early Pub Date

February 27, 2026

Publication Date

February 27, 2026

Submission Date

September 2, 2025

Acceptance Date

January 26, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.33988/auvfd.1774791

IZ

https://izlik.org/JA79AW25TT

Cite

RIS / Bibtex

APA

Sarıçam İnce, S., & Akan, M. (2026). First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Advanced Online Publication, 1-6. https://doi.org/10.33988/auvfd.1774791

AMA

1.Sarıçam İnce S, Akan M. First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye. Ankara Univ Vet Fak Derg. 2026;(Advanced Online Publication):1-6. doi:10.33988/auvfd.1774791

Chicago

Sarıçam İnce, Seyyide, and Mehmet Akan. 2026. “First Report of BlaOXA and Mcr-2 Genes Co-Existing in a Multidrug-Resistant Pseudomonas Aeruginosa Isolate Recovered from a Layer Chicken Flock in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication: 1-6. https://doi.org/10.33988/auvfd.1774791.

EndNote

Sarıçam İnce S, Akan M (February 1, 2026) First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi Advanced Online Publication 1–6.

IEEE

[1]S. Sarıçam İnce and M. Akan, “First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye”, Ankara Univ Vet Fak Derg, no. Advanced Online Publication, pp. 1–6, Feb. 2026, doi: 10.33988/auvfd.1774791.

ISNAD

Sarıçam İnce, Seyyide - Akan, Mehmet. “First Report of BlaOXA and Mcr-2 Genes Co-Existing in a Multidrug-Resistant Pseudomonas Aeruginosa Isolate Recovered from a Layer Chicken Flock in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi. Advanced Online Publication (February 1, 2026): 1-6. https://doi.org/10.33988/auvfd.1774791.

JAMA

1.Sarıçam İnce S, Akan M. First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye. Ankara Univ Vet Fak Derg. 2026;:1–6.

MLA

Sarıçam İnce, Seyyide, and Mehmet Akan. “First Report of BlaOXA and Mcr-2 Genes Co-Existing in a Multidrug-Resistant Pseudomonas Aeruginosa Isolate Recovered from a Layer Chicken Flock in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication, Feb. 2026, pp. 1-6, doi:10.33988/auvfd.1774791.

Vancouver

1.Seyyide Sarıçam İnce, Mehmet Akan. First report of blaOXA and mcr-2 genes co-existing in a multidrug-resistant Pseudomonas aeruginosa isolate recovered from a layer chicken flock in Türkiye. Ankara Univ Vet Fak Derg. 2026 Feb. 1;(Advanced Online Publication):1-6. doi:10.33988/auvfd.1774791