Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye

Gökben Özbakış Beceriklisoy; Emin Karakurt; Bengi Çınar; Serpil Dağ; Enver Beytut; Hilmi Nuhoğlu; Ayfer Yıldız

doi:10.33988/auvfd.1842868

Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye

Abstract

Coenurosis, caused by the metacestode (Coenurus cerebralis) of Taenia multiceps, is a significant zoonotic parasitic disease that primarily affects the small ruminants, resulting in economic difficulties within the livestock industry. This study first aimed to evaluate the expression of cytokines, including Interleukin (IL)-1β, 2, 6, 8, 10, 12, Tumor Necrosis Factor Alpha (TNF-α) and Interferon Gamma (IFN-γ) via immunohistochemical methods and the second objective was to performed molecular analyses including phylogenetic and haplotype assessments of the mitochondrial cytochrome c oxidase subunit 1 (COX1) and NADH dehydrogenase subunit 1 (ND1) genes of C. cerebralis isolates obtained from sheep in Türkiye. Ten sheep brains infected with C. cerebralis were examined. Foreign body granulomatous reactions were detected in six animals. All animals were recorded positive for immunohistochemically for TNF-α, IFN-γ, and Interleukins. Molecular analysis of the COX1 and ND1 genes positive results were obtained in twelve cysts from ten animals. The isolates revealed four different haplotypes out of 30 haplotypes for the COX1 gene and 9 distinct haplotypes (six unique) out of 46 for the ND1 gene. Among the two animals with multiple C. cerebralis cysts, ND1 sequencing revealed four distinct haplotypes, whereas COX1 sequencing showed that one of these animals harboured two distinct haplotypes. When potential relationships between the IHC results and the haplotype distributions of the cases were examined, no consistent association emerged between particular lineages and granuloma formation, clinical findings or cytokine expression profiles. These findings enhance the understanding of cytokine expression patterns and genetic diversity of C. cerebralis in Türkiye.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Parasitology, Veterinary Pathology

Journal Section

Research Article

Authors

Gökben Özbakış Beceriklisoy ^*
0000-0002-3354-9150
Türkiye

Emin Karakurt
0000-0003-2019-3690
Türkiye

Bengi Çınar
0000-0002-8955-0097
Türkiye

Serpil Dağ
0000-0001-7667-689X
Türkiye

Enver Beytut
0000-0003-3360-2940
Türkiye

Hilmi Nuhoğlu
0000-0003-2530-2542
Türkiye

Ayfer Yıldız
0000-0002-6569-5435
Türkiye

Early Pub Date

September 18, 2026

Publication Date

-

Submission Date

December 15, 2025

Acceptance Date

February 16, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.33988/auvfd.1842868

IZ

https://izlik.org/JA46KK64ZH

Cite

RIS / Bibtex

APA

Özbakış Beceriklisoy, G., Karakurt, E., Çınar, B., Dağ, S., Beytut, E., Nuhoğlu, H., & Yıldız, A. (2026). Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Advanced Online Publication. https://doi.org/10.33988/auvfd.1842868

AMA

1.Özbakış Beceriklisoy G, Karakurt E, Çınar B, et al. Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye. Ankara Univ Vet Fak Derg. 2026;(Advanced Online Publication). doi:10.33988/auvfd.1842868

Chicago

Özbakış Beceriklisoy, Gökben, Emin Karakurt, Bengi Çınar, et al. 2026. “Cytokine Profiles and Genetic Variations Based on Mitochondrial ND1 and COX1 Genes of Coenurus Cerebralis in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication. https://doi.org/10.33988/auvfd.1842868.

EndNote

Özbakış Beceriklisoy G, Karakurt E, Çınar B, Dağ S, Beytut E, Nuhoğlu H, Yıldız A (September 1, 2026) Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye. Ankara Üniversitesi Veteriner Fakültesi Dergisi Advanced Online Publication

IEEE

[1]G. Özbakış Beceriklisoy et al., “Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye”, Ankara Univ Vet Fak Derg, no. Advanced Online Publication, Sept. 2026, doi: 10.33988/auvfd.1842868.

ISNAD

Özbakış Beceriklisoy, Gökben - Karakurt, Emin - Çınar, Bengi - Dağ, Serpil - Beytut, Enver - Nuhoğlu, Hilmi - Yıldız, Ayfer. “Cytokine Profiles and Genetic Variations Based on Mitochondrial ND1 and COX1 Genes of Coenurus Cerebralis in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi. Advanced Online Publication (September 1, 2026). https://doi.org/10.33988/auvfd.1842868.

JAMA

1.Özbakış Beceriklisoy G, Karakurt E, Çınar B, Dağ S, Beytut E, Nuhoğlu H, Yıldız A. Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye. Ankara Univ Vet Fak Derg. 2026. doi:10.33988/auvfd.1842868.

MLA

Özbakış Beceriklisoy, Gökben, et al. “Cytokine Profiles and Genetic Variations Based on Mitochondrial ND1 and COX1 Genes of Coenurus Cerebralis in Türkiye”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication, Sept. 2026, doi:10.33988/auvfd.1842868.

Vancouver

1.Gökben Özbakış Beceriklisoy, Emin Karakurt, Bengi Çınar, Serpil Dağ, Enver Beytut, Hilmi Nuhoğlu, Ayfer Yıldız. Cytokine profiles and genetic variations based on mitochondrial ND1 and COX1 genes of Coenurus cerebralis in Türkiye. Ankara Univ Vet Fak Derg. 2026 Sep. 1;(Advanced Online Publication). doi:10.33988/auvfd.1842868