Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows

Burcu Menekşe Balkan; Öğünç Meral; Güzin Özkurt

doi:10.33988/auvfd.1882481

Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows

Abstract

Thiol/disulfide homeostasis is a key component of the antioxidant defense system and studies on the determination and improvement of thiol/disulfide homeostasis may contribute to the prevention of the adverse effects of oxidative stress on animal production, and welfare. The purpose of this study is to investigate the total thiol, native thiol and disulfide levels, together with total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), paraoxonase-1 (PON1) activity and lipid profile in Simental and Montofon dairy cows across different seasons. Blood samples were collected from animals in May and November. Serum concentrations of total thiol, native thiol, disulfide, TAS, TOS and PON1 were measured using colorimetric methods. Concentrations of triglycerides (TG), total cholesterol (TC), HDL, and LDL were measured using an automated analyzer. In both breeds, serum total thiol and disulfide levels increased in November compared with May, whereas native thiol levels decreased in November. In both the Simental and Montofon breeds, serum TOS and OSI levels increased in November compared to May, while TAS levels decreased. Also, serum HDL, LDL and cholesterol levels increased in both breeds, while PON1 levels increased only in Simental in November compared to May. According to our results, both breeds were affected by seasonal changes, but Simental cows were more sensitive than Montofon cows. Research on thiol/disulfide homeostasis in veterinary medicine is limited and this study is the first to evaluate thiol/disulfide homeostasis along with oxidative stress and lipid profiles in dairy cows across different seasons.

Keywords

Thanks

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References

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Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Burcu Menekşe Balkan
0000-0002-0206-6455
Türkiye

Öğünç Meral ^*
0000-0001-8813-4991
Türkiye

Güzin Özkurt
0000-0003-2732-9490
Türkiye

Early Pub Date

May 22, 2026

Publication Date

-

Submission Date

February 6, 2026

Acceptance Date

April 7, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.33988/auvfd.1882481

IZ

https://izlik.org/JA54ZW47CT

Cite

RIS / Bibtex

APA

Balkan, B. M., Meral, Ö., & Özkurt, G. (2026). Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Advanced Online Publication. https://doi.org/10.33988/auvfd.1882481

AMA

1.Balkan BM, Meral Ö, Özkurt G. Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows. Ankara Univ Vet Fak Derg. 2026;(Advanced Online Publication). doi:10.33988/auvfd.1882481

Chicago

Balkan, Burcu Menekşe, Öğünç Meral, and Güzin Özkurt. 2026. “Seasonal Changes in Thiol–disulfide Homeostasis, Oxidant–antioxidant Status, and Lipid Profile in Healthy Dairy Cows”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication. https://doi.org/10.33988/auvfd.1882481.

EndNote

Balkan BM, Meral Ö, Özkurt G (May 1, 2026) Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows. Ankara Üniversitesi Veteriner Fakültesi Dergisi Advanced Online Publication

IEEE

[1]B. M. Balkan, Ö. Meral, and G. Özkurt, “Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows”, Ankara Univ Vet Fak Derg, no. Advanced Online Publication, May 2026, doi: 10.33988/auvfd.1882481.

ISNAD

Balkan, Burcu Menekşe - Meral, Öğünç - Özkurt, Güzin. “Seasonal Changes in Thiol–disulfide Homeostasis, Oxidant–antioxidant Status, and Lipid Profile in Healthy Dairy Cows”. Ankara Üniversitesi Veteriner Fakültesi Dergisi. Advanced Online Publication (May 1, 2026). https://doi.org/10.33988/auvfd.1882481.

JAMA

1.Balkan BM, Meral Ö, Özkurt G. Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows. Ankara Univ Vet Fak Derg. 2026. doi:10.33988/auvfd.1882481.

MLA

Balkan, Burcu Menekşe, et al. “Seasonal Changes in Thiol–disulfide Homeostasis, Oxidant–antioxidant Status, and Lipid Profile in Healthy Dairy Cows”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication, May 2026, doi:10.33988/auvfd.1882481.

Vancouver

1.Burcu Menekşe Balkan, Öğünç Meral, Güzin Özkurt. Seasonal changes in thiol–disulfide homeostasis, oxidant–antioxidant status, and lipid profile in healthy dairy cows. Ankara Univ Vet Fak Derg. 2026 May 1;(Advanced Online Publication). doi:10.33988/auvfd.1882481