Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats

Bayram Süzer; İlker Arıcan; Ezgi Yumuşak; Ahmet Akkoç; Murat Yalçın

doi:10.33988/auvfd.1910833

Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats

Abstract

The gut microbiota plays a crucial role in regulating host metabolism and homeostasis through immune, endocrine, and metabolic pathways. This study investigated the impact of antibiotic-induced microbial disruption during the critical post-weaning period on bone biomechanical properties and mineral composition and evaluated whether fecal microbiota transplantation (FMT) could reverse these alterations in rats. Following weaning, Sprague Dawley rats were allocated to control (CON), dysbiosis (DYS), and FMT groups. To induce experimental intestinal dysbiosis, rats in the DYS and FMT groups were administered a broad-spectrum antibiotic mixture for 14 days following an established protocol. Subsequently, the FMT group received daily fecal suspensions from healthy donor rats for 10 days. At 8 weeks of age, tibial biomechanical integrity was assessed via three-point bending tests, and bone mineral composition was determined through crude ash, calcium (Ca), and phosphorus (P) analyses. The results demonstrated that antibiotic-induced DYS significantly reduced tibial ultimate force and stiffness while increasing displacement, indicating impaired bone mechanical integrity. In addition, DYS significantly decreased crude bone ash content as well as Ca and P levels. While histopathological examination did not reveal marked structural alterations among the groups, the FMT intervention partially restored bone mechanical strength and mineral composition toward control levels. These findings suggest that antibiotic-induced perturbations during early development negatively affect bone mineralization and biomechanics, while the partial recovery following FMT supports the concept of a functional gut–bone axis.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Anatomy and Physiology

Journal Section

Research Article

Authors

Bayram Süzer
0000-0002-2687-1221
Türkiye

İlker Arıcan
0000-0001-6342-0094
Türkiye

Ezgi Yumuşak
0000-0002-7773-3419
Türkiye

Ahmet Akkoç
0000-0002-5090-7917
Türkiye

Murat Yalçın ^*
0000-0002-5600-8162
Türkiye

Early Pub Date

August 20, 2026

Publication Date

-

Submission Date

March 16, 2026

Acceptance Date

July 29, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.33988/auvfd.1910833

IZ

https://izlik.org/JA42KZ75TY

Cite

RIS / Bibtex

APA

Süzer, B., Arıcan, İ., Yumuşak, E., Akkoç, A., & Yalçın, M. (2026). Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Advanced Online Publication. https://doi.org/10.33988/auvfd.1910833

AMA

1.Süzer B, Arıcan İ, Yumuşak E, Akkoç A, Yalçın M. Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats. Ankara Univ Vet Fak Derg. 2026;(Advanced Online Publication). doi:10.33988/auvfd.1910833

Chicago

Süzer, Bayram, İlker Arıcan, Ezgi Yumuşak, Ahmet Akkoç, and Murat Yalçın. 2026. “Impact of Antibiotic-Induced Gut Dysbiosis Model on Bone Mineralization and Biomechanical Properties in Post-Weaning Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication. https://doi.org/10.33988/auvfd.1910833.

EndNote

Süzer B, Arıcan İ, Yumuşak E, Akkoç A, Yalçın M (August 1, 2026) Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi Advanced Online Publication

IEEE

[1]B. Süzer, İ. Arıcan, E. Yumuşak, A. Akkoç, and M. Yalçın, “Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats”, Ankara Univ Vet Fak Derg, no. Advanced Online Publication, Aug. 2026, doi: 10.33988/auvfd.1910833.

ISNAD

Süzer, Bayram - Arıcan, İlker - Yumuşak, Ezgi - Akkoç, Ahmet - Yalçın, Murat. “Impact of Antibiotic-Induced Gut Dysbiosis Model on Bone Mineralization and Biomechanical Properties in Post-Weaning Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi. Advanced Online Publication (August 1, 2026). https://doi.org/10.33988/auvfd.1910833.

JAMA

1.Süzer B, Arıcan İ, Yumuşak E, Akkoç A, Yalçın M. Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats. Ankara Univ Vet Fak Derg. 2026. doi:10.33988/auvfd.1910833.

MLA

Süzer, Bayram, et al. “Impact of Antibiotic-Induced Gut Dysbiosis Model on Bone Mineralization and Biomechanical Properties in Post-Weaning Rats”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication, Aug. 2026, doi:10.33988/auvfd.1910833.

Vancouver

1.Bayram Süzer, İlker Arıcan, Ezgi Yumuşak, Ahmet Akkoç, Murat Yalçın. Impact of antibiotic-induced gut dysbiosis model on bone mineralization and biomechanical properties in post-weaning rats. Ankara Univ Vet Fak Derg. 2026 Aug. 1;(Advanced Online Publication). doi:10.33988/auvfd.1910833