Detection of central nervous system tissues in minced meat by ELISA and histological examination

Okan Bora Nazik; Nesrin Özfiliz

doi:10.33988/auvfd.1982069

Detection of central nervous system tissues in minced meat by ELISA and histological examination

Abstract

The aim of this study was to compare two histological staining methods and an ELISA for the detection of central nervous system (CNS) tissue in raw minced meat obtained from tissues surrounding the spinal cord of a bovine carcass split using a band saw, as well as in experimentally prepared fresh minced meat samples containing different proportions of brain or spinal cord tissue. Minced meat samples containing CNS tissue at concentrations ranging from 0.1% to 10% were analyzed using Crossmon’s modified triple staining, Kluver–Barrera staining, and ELISA. No statistically significant differences were detected between the staining methods or between histological examination and ELISA. However, ELISA detected CNS tissue at a lower concentration (0.1%) than histological examination (0.2%), indicating greater analytical sensitivity. In contrast, histological examination enabled direct visualization of tissue morphology, including ganglion tissue that was not detected by ELISA. Thus, the two techniques provided complementary information. These findings may facilitate the identification of adulteration in meat and meat products and help prevent the consumption of CNS tissues that may pose a public health risk associated with bovine spongiform encephalopathy (BSE).

Keywords

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Details

Primary Language

English

Subjects

Veterinary Histology and Embryology

Journal Section

Research Article

Authors

Okan Bora Nazik
0000-0002-5547-4823
Türkiye

Nesrin Özfiliz ^*
0000-0002-9552-0214
Türkiye

Early Pub Date

September 18, 2026

Publication Date

October 1, 2026

Submission Date

June 30, 2026

Acceptance Date

August 27, 2026

Published in Issue

Year 2026 Volume: 73 Number: 4

DOI

https://doi.org/10.33988/auvfd.1982069

IZ

https://izlik.org/JA35HM28JA

Cite

RIS / Bibtex

APA

Nazik, O. B., & Özfiliz, N. (2026). Detection of central nervous system tissues in minced meat by ELISA and histological examination. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 73(4), 482-489. https://doi.org/10.33988/auvfd.1982069

AMA

1.Nazik OB, Özfiliz N. Detection of central nervous system tissues in minced meat by ELISA and histological examination. Ankara Univ Vet Fak Derg. 2026;73(4):482-489. doi:10.33988/auvfd.1982069

Chicago

Nazik, Okan Bora, and Nesrin Özfiliz. 2026. “Detection of Central Nervous System Tissues in Minced Meat by ELISA and Histological Examination”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 (4): 482-89. https://doi.org/10.33988/auvfd.1982069.

EndNote

Nazik OB, Özfiliz N (October 1, 2026) Detection of central nervous system tissues in minced meat by ELISA and histological examination. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73 4 482–489.

IEEE

[1]O. B. Nazik and N. Özfiliz, “Detection of central nervous system tissues in minced meat by ELISA and histological examination”, Ankara Univ Vet Fak Derg, vol. 73, no. 4, pp. 482–489, Oct. 2026, doi: 10.33988/auvfd.1982069.

ISNAD

Nazik, Okan Bora - Özfiliz, Nesrin. “Detection of Central Nervous System Tissues in Minced Meat by ELISA and Histological Examination”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 73/4 (October 1, 2026): 482-489. https://doi.org/10.33988/auvfd.1982069.

JAMA

1.Nazik OB, Özfiliz N. Detection of central nervous system tissues in minced meat by ELISA and histological examination. Ankara Univ Vet Fak Derg. 2026;73:482–489.

MLA

Nazik, Okan Bora, and Nesrin Özfiliz. “Detection of Central Nervous System Tissues in Minced Meat by ELISA and Histological Examination”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 73, no. 4, Oct. 2026, pp. 482-9, doi:10.33988/auvfd.1982069.

Vancouver

1.Okan Bora Nazik, Nesrin Özfiliz. Detection of central nervous system tissues in minced meat by ELISA and histological examination. Ankara Univ Vet Fak Derg. 2026 Oct. 1;73(4):482-9. doi:10.33988/auvfd.1982069