Protective effects of resveratrol on testicular oxidative stress induced of MK-801 in mice

Abstract

Recently, it has been discovered that the doses of the MK-801 causing schizophrenia also initiate the oxidative stress in the testis. The current study investigated the protective role of the resveratrol against the MK-801 induced oxidative stress in the testis in mice. The testis weight, the total oxidant-antioxidant status, seminiferous tubules diameter, epithelial height, testicular pathology, and epididymal sperm motility were evaluated. A total of 24 male mice were equally divided into 4 groups so that each group included 6 mice. In the study, Group I (control group) was intraperitoneally received with 0.9% saline (10ml/kg). Group II was i.p. administered with MK-801 (1mg/kg), Group III was treated with i.p. MK-801 (1mg/kg) and resveratrol (40mg/kg), and the Group IV was treated i.p. with resveratrol (40mg/kg). All injections were performed for 14 days. According to the results, in the testis of mice in Group II the oxidative stress was observed. The oxidative stress affected the seminiferous tubules pathologically and decreased the weight of the testis and sperm motility. However, resveratrol protected the MK-801 induced oxidative stress in the testis. Moreover, this dose of the resveratrol increased the sperm motility compared with the controls. In conclusion, MK-801 caused oxidative stress in the testis and resveratrol had a protective effect against this damage.

Keywords

• MK-801,oxidative stress,resveratrol,testis,schizoprenia

Fare testislerinde Mk-801’le indüklenen oksidatif strese karşı resveratrolün koruyucu etkisi

Abstract

Günümüzde MK-801’in şizofreniye sebep olan dozlarının testiste oksidatif strese de neden olduğu ortaya çıkmıştır. Bu çalışma fare testislerinde MK-801 kullanılarak ortaya çıkartılan oksidatif strese karşı resveratrolün koruyucu etkisini araştırmak amacıyla yapılmıştır. Testisler ağırlık, toplam oksidan-antioksidan seviyeleri, seminifer tübüllerin çapları ve epitel dokularının yükseklikleri, patolojik ve epididimal sperm motilitesi yönünden değerlendirilmiştir. Bu amaçla 24 fare alınarak grup başına 6’şar fareden oluşan 4 grup oluşturulmuştur. Grup I, kontrolden oluşmuştur ve bu gruba intraperitoneal yolla %0,9 FTS (10ml/kg) verilmiştir. Grup II’ye i.p. yolla MK-801 (1mg/kg), Grup III’e i.p. yolla MK-801 (1mg/kg) ve resveratrol (40mg/kg), Grup IV’e de i.p. olarak resveratrol (40mg/kg) verilmiştir. Uygulamalar on dört gün boyunca sürmüştür. Bu sürenin sonunda testisler çıkartılmış ve yapılan incelemeler neticesinde testiste ikinci grupta MK-801’in oksidatif strese sebep olduğu saptanmış, testis ağırlığında azalma, sperm motilitesinde düşme ve tübüllerde patolojik değişiklikler olduğu görülmüştür. Resveratrolün ise oksidatif strese karşı testisi koruduğu ortaya konulmuştur. Ayrıca resveratrol grubunda kontrole göre sperm motilitesinin arttığı gözlenmiştir. Sonuç olarak MK-801’in testiste oksidatif strese sebep olduğu ve resveratrolün de koruyucu etkisinin bulunduğu ortaya çıkarılmıştır.

Keywords

• MK-801,oksidatif stres,resveratrol,testis,şizofreni

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