Sağlıklı tavşanlarda Encephalitozoon cuniculi seropozitifliği ile böbrek biyokimyasal belirteçleri arasındaki ilişki

Abstract

Encephalitozoon cuniculi, özellikle tavşangillerde ve çeşitli ülkelerdeki birçok yaban ve evcil hayvanlarda latent hastalığa neden olabilmektedir. Bu enfeksiyon potansiyel olarak zoonotik ve fırsatçı bir patojenden kaynaklandığından veteriner ve halk sağlığı için önemlidir. Bu çalışmanın amacı, klinik olarak sağlıklı tavşanlarda E. cuniculi seropozitifliği ve böbrek fonksiyon belirteçleri arasındaki ilişkinin, hastalığın tanısında yararlı olabileceğini araştırmaktır. Bu çalışmada, sağlıklı tavşanlarda E. cuniculi enfeksiyonunun seropozitifliği enzim bağlantılı immünosorbent testi (ELISA) ile belirlenmiş ve böbrek fonksiyon test sonuçları ile otopsi bulguları birlikte değerlendirilmiştir. Bir laboratuvar tavşan yetiştirme tesisinde, ELISA testi, 97 tavşanın 48'inin (%49.5) E. cuniculi'ye karşı seropozitif olduğunu göstermiştir. Seropozitif hayvanlarda kan üre nitrojen (BUN) ve kreatinin düzeyleri anlamlı olarak yüksek bulunmuştur. Serum kreatinin (p<0.001) ve BUN (p<0.01) düzeylerinin tavşanların serolojik durumu ile istatistik olarak anlamlı bir ilişkisi olduğu anlaşılmıştır. Enfeksiyonun doğrulanması için iki seropozitif tavşan otopsi edildi. Böbrekteki histopatolojik bulgulara göre tübül epitelinde dejeneratif değişiklikler ve E. cuniculi sporları tespit edilmiştir. Encephalitozoon cuniculi enfeksiyonunu teşhis etmek için tavşanlarda serolojik ve histopatolojik yöntemler rutin olarak kullanılmamaktadır. Böbrekler tavşanlarda ensefalitozoonosisde en çok etkilenen organlardan biridir. Çalışmanın da gösterdiği üzere, kan üre nitrojen ve kreatinin düzeylerinin belirlenmesi, seropozitif tavşanların böbrek fonksiyonlarının değerlendirilmes, klinik ve genel sağlık durumunu yorumlanması adına yararlı olabileceği anlaşılmaktadır.

Keywords

• Biyokimyasal belirteçler,böbrek fonksiyonu,Encephalitozoon cuniculi,tavşan

Relationship between seropositivity of Encephalitozoon cuniculi and renal biochemical markers in clinically healthy rabbits

Abstract

Encephalitozoon cuniculi can cause latent disease, especially in lagomorphs and many wild and domestic animals in various countries. This infection is important for veterinary and public health because it is caused by a potentially zoonotic and opportunistic pathogen. The aim of this study was to investigate the relationship between seropositivity of E. cuniculi and renal function markers, which may be useful in predicting the disease in clinically healthy rabbits. In this study, the seropositivity of E. cuniculi infection in clinically healthy rabbits was determined, and necropsy findings were evaluated along with the results of renal function tests. In a laboratory rabbit breeding facility, enzyme-linked immunosorbent assay (ELISA) tests showed that 48 (49.5%) of 97 rabbits were seropositive against E. cuniculi. Blood urea nitrogen (BUN) and creatinine levels were significantly elevated in seropositive animals. Two seropositive rabbits were necropsied to confirm the infection. According to histopathological findings in the kidney, degenerative changes and E. cuniculi spores were identified in the tubule epithelia. Serum creatinine (p<0.001) and BUN (p<0.01) levels were found to have a statistically significant relationship with the serological status of rabbits. Serological and histopathological methods are not routinely used in rabbits to diagnose E. cuniculi infection. The kidneys were one of the most affected organs in encephalitozoonosis in rabbits. As revealed in this study, the testing blood urea nitrogen and creatinine levels would be useful for the evaluation of general health status and renal function of the seropositive rabbits, and clinical interpretation as well.

Keywords

• Encephalitozoon cuniculi,Biochemical markers,Rabbit,Renal function

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