Kuzu enteritislerinde Cryptosporidiosis hastalığının patolojik yöntemlerle araştırılması

Abstract

The aim of this study is to determine C. parvum in small intestinal samples of lambs (up to 30 days old) with enteritis. For this aim, fresh intestinal smears prepared from 60 lambs (euthanized due to diarrhea) and stained with Modified Ziehl-Neelsen (MZN). In addition to these 60 fresh samples, 85 different small intestinal samples obtained from dead lambs (total 145 small intestinal samples) were embedded in paraffin for histopathology, immunofluorescence (IF) and immunohistochemistry (IHC). Slides were stained according to protocols (MZN, hematoxylin-eosin, IHC and IF). In MZN staining, 13.3% of smear samples were found positive for Cryptosporidium spp. Besides, C. parvum positivity was detected in the rate of 13.8% in IF and IHC stains. In the histopathologic examination, desquamation and necrosis of intestinal epithelium, villus atrophy, Cryptosporidium spp. along the surface of villus epithelium, hyperemia, mononuclear cellular infiltration, mitosis in crypt epithelium, intraepithelial lymphocytes, dilatation in the lymphatic canal and Eimeria spp. in some cases were observed in varying degree. In conclusion, IF, IHC and MZN stains were found effective in revealing cryptosporidiosis in lambs.

Keywords

• enteritis,histopathology,immunofluorescence,C. parvum,Cryptosporidiosis,immunohistochemistry

Kuzu enteritislerinde Cryptosporidiosis hastalığının patolojik yöntemlerle araştırılması

Abstract

Çalışmanın amacı kuzu ishallerinde önemli bir patojen olan Cryptosporidium parvum (C. parvum) etkenlerinin bağırsak smearı ve parafin kesitlerden, Modifiye Ziehl-Neelsen (MZN), histopatoloji, immunohistokimya (İHK) ve immunfloresan (İF) boyama yöntemleri ile belirlemek ve Cryptosporidiosis hastalığının 30 günlüğe kadar olan kuzulardaki yaygınlığını ortaya koymaktır. Bu amaçla, ishal belirtisi gösteren ve agoni halde iken nekropsi yapılmış 60 adet ve yine ishal belirtisiyle ölmüş 85 adet (toplam 145 adet) kuzudan alınan bağırsak örnekleri kullanılmıştır. MZN boyama sadece, agoni halde iken nekropsileri yapılan 60 kuzudan alınan taze incebağırsak örneklerinden hazırlanan smear örneklerine yapılmıştır. Bu 60 örnekle birlikte ölü olarak getirilen 85 kuzudan (toplam 145) elde edilen incebağırsak örneklerinden hazırlanan parafin kesitlerden ise histopatoloji, İF ve İHC boyamaları prosedürüne uygun olarak yapıldı. MZN boyamalarda (60 örnek) %13.3 oranında Cryptosporidium spp., parafin blokları hazırlanan toplam 145 örnekten alınan kesitlerin IF ve IHC boyamalarda ise %13.8 oranında C. parvum pozitif reaksiyon gözlenmiştir. Etken tespit edilen bağırsak kesitlerinin histopatolojik incelemelerinde; değişen derecelerde olmak üzere; epitel hücrelerinde nekroz, deskuamasyon ve villuslarda kütleşme, epitel yüzeyi boyunca Cryptosporidium spp. etkenleri, propria mukozada hiperemi ve mononükleer hücre infiltrasyonu, kript epitelinde mitoz artışı, lenfatik kanalda genişleme, intraepitelyal lenfositler ve bazı örneklerde Eimeria spp gözlendi. Sonuç olarak çalışmada İF, İHC ve MZN boyamalarının kuzularda Cryptosporidiosis hastalığını ortaya koymada etkili olduğu görülmüştür.

Keywords

• C. parvum,Cryptosporidiosis,enteritis,histopatoloji,immunfloresan,immunohistokimya

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