The association between insulin-like growth factors I, II and bovine papillomavirus type-1 expressions in naturally occuring bovine fibropapilloma cases

Abstract

In this study, we hypothesized that insulin-like growth factors (IGFs) may have a combine effect together with BPV-1 in the etiopathogenesis of fibropapilloma. With this aim, macroscopic and histopathological findings of the fibropapillomatous changes were described in masses collected from the skin of neck, hindlimbs and abdominal region of 15 cattle, ranging from 14 to 23 months old. In several cases, hyperkeratosis (n=7) and/or parakeratosis (n=8), acanthosis (n=15), degenerative changes (n=15) and inflammatory cell infiltrations (n=9), and also vascularization (n=15) were accompanied to the neoplastic cells comprising paranchyme and stroma. In terms of virus entity in collected samples, L1 and L2 genes of BPV-1 were found to be positive by PCR. Immunohistochemically, BPV-1, IGF-I and IGF II expressions were found parallel. IGF-I positivities were stronger and more common in the epidermal cells and connective tissue components when compared with IGF-II positivity. In general, immunolocalization of both IGF-I and IGF-II were seen in the cytoplasm and membranes of all cell layers in the epidermis and only in the fibrocytes and fibroblasts in addition to collagen bundles. We concluded that IGF-I and IGF-II could have an effect as much as BPV-1 in pathogenesis of fibropapillomatous changes in the epidermis and dermis

Keywords

• Bovine fibropapilloma
• bovine papillomavirus type-1
• insulin-like growth factors

Doğal gelişen sığır fibropapilloma olgularında insülin-benzeri büyüme faktörü I, II ve sığırpapillomavirus tip-1 ekspresyonları arasındaki ilişki

Abstract

Bu çalışmada, insülin benzeri büyüme faktörlerinin (IGF'ler) fibropapillomanın etiyopatogenezinde BPV-1 ile birlikte kombine bir etkisinin olabileceği ileri sürülmüştür. Bu amaçla, yaşları 14-23 ay arasında değişen toplam 15 sığırda boyun, arka bacaklar ve abdominal bölgede fibropapillomatöz değişikliklere ait makroskopik ve histopatolojik bulgular tanımlanmıştır. Tümörün paranşim ve stromasını oluşturan neoplazik hücrelere çeşitli olgularda hiperkeratozis (n=7), ve/veya parakeratozis (n=8), acanthosis (n=15), dejeneratif değişiklikler (n=15), yangısal hücre infiltrasyonları (n=9) ve vaskülarizasyon (n=15)'un eşlik ettiği dikkati çekmiştir. Toplanan örneklerde virusun varlığı, BPV-1'in L1 ve L2 genlerine PCR ile bakılarak pozitif olduğu bulunmuştur. İmmunohistokimyasal olarak BPV-1, IGF-I ve IGF-II ekspresyonlarının birbirine paralel olduğu belirlenmiştir. IGF-I pozitivitelerinin, IGF-II ile karşılaştırıldığında epidermal ve bağ doku hücrelerinde daha şiddetli ve yaygın olduğu gözlenmiştir. Hem IGF-I hem de IGF-II için immunpozitiflikler genelde epidermisin tüm katmanlarındaki hücrelerin sitoplazma ve membranlarında, kollajen demetlerin yanı sıra fibrosit ile fibroblastlarda gözlenmiştir. Fibropapillomalarda epidermis ve dermisteki fibropapillomatöz değişikliklerin patogenezisinde IGF-I and IGF-II'nin en az BPV-1 kadar etkili olabileceği sonucuna varılmıştır

Keywords

• İnsulin-benzeri büyüme faktörleri
• sığır fibropapillomu
• sığır papillomavirus tip-1

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