Lipid peroxidation level and antioxidant enzyme activities in septicemic calves

Abstract

In recent years, studies showed that there is a close relationship between oxidative stress and pathogenesis of the disease. The aim of the study is to determine the serum malondialdehyde (MDA) levels for lipid peroxidation status and serum glutathione peroxidase (GPx), superoxide dismutase (SOD), total bilirubin and albumin levels for antioxidant activities to find out the possible relationship between oxidative stress and septicemia in calves. The materials of this study are 40 septicemic and 13 healthy calves. No significant differences were observed for serum MDA and albumin levels between septicemic and healthy groups (p>0.05). Septicemic calves had significantly higher levels of GPx, SOD and total bilirubin as compared to the controls (p<0.001). The results obtained from this study suggest that septicemia in calves induced changes of antioxidant enzymes activity. The data obtained from this study indicates that oxidative stress might play important roles in the pathogenesis of septicemia in calves

Keywords

• Glutathione peroxidase
• neonatal septicemia
• oxidative stress
• superoxide dismutase

Septisemili buzağılarda lipid peroksidasyon düzeyi ve antioksidan enzim aktiviteleri

Abstract

Oksidatif stres ile hastalıkların patogenezi arasındaki ilişki son yıllarda gerçekleştirilen değişik çalışmalarla açıklanmaya çalışılmıştır. Bu çalışmada septisemili buzağılarda lipid peroksidasyon ürünü olan serum malondialdehit (MDA) ve antioksidan savunma sisteminin parametreleri olan serum glutatyon peroksidaz (GPx), süperoksit dismutaz (SOD) enzimleri ile total bilirubin ve albumin düzeylerinin belirlenerek neonatal septisemi ile ilişkisinin gösterilmesi amaçlanmıştır. Çalışmanın materyalini 13 sağlıklı ve 40 septisemili buzağı serum örnekleri oluşturmaktadır. Septisemili grup ile kontrol grubu arasında serum MDA ve albümin düzeyleri arasında fark bulunamamıştır (p>0.05). Septisemili buzağıların serum GPx, SOD ve total bilirubin düzeylerindeki artış kontrol grubu ile karşılaştırıldığında istatistiksel olarak anlamlı bulunmuştur (p<0.001). Elde edilen bu bulgular buzağılarda görülen septiseminin patogenezinde oksidatif stresin rolü olduğu kanaatini oluşturmaktadır

Keywords

• Glutatyon peroksidaz
• neonatal septisemi
• oksidatif stres
• süperoksit dismutaz

References

1. Ayala A, Munoz MF, Argüelles S (2014): Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev, 2014, 360438.
2. Basoglu A, Sen I, Sevinc M, Simsek A (2004): Serum concentrations of tumor necrosis factor-alpha in neonatal calves with presumed septicemia. J Vet Intern Med, 18, 238- 241.
3. Batra S, Kumar R, Kapoor AK ve ark. (2000): Alterations in antioxidant status during neonatal sepsis. Ann Trop Paediatr, 20, 27-33.
4. Benzer F, Ozan ST (2003): Fasciola hepatica ile enfekte koyunlarda lipid peroksidasyonu, antioksidant enzimler ve nitrik oksit düzeyleri. Turk J Vet Anim Sci, 27, 657-661.
5. Celi P (2011): Biomarkers of oxidative stress in ruminant medicine. Immunopharmacol Immunotoxicol, 33, 233-240.
6. Czerska M, Mikolajewska K, Zielinski M ve ark. (2015): Today's oxidative stress markers. Med Pr, 66, 393-405.
7. Davutoglu M, Guler E, Olgar S ve ark. (2008): Oxidative stress hyperbilirubinemia. Saudi Med J, 29, 1743-1748. status in neonatal
8. Değer Y, Ertekin A, Değer S ve ark. (2008): Lipid peroxidation and antioxidant potential of sheep liver ınfected naturally with distomatosis. Türkiye Parazitoloji Dergisi, 32, 23-26.

9. Ercan N, Fidancı UR (2012): Piyodermalı köpeklerde idrarda 8-hidroksi-2’ deoksiguanozin (8-OHdG) düzeyleri. Ankara Univ Vet Fak Derg, 59, 163-168.
10. Ercan N, Tuzcu N, Başbug O ve ark. (2014): The evaluation of ımportant biomarkers in healthy cattle. Kafkas Üniv Vet Fak Derg, 20, 749-755.
11. Fecteau G, Van Metre DC, Pare J ve ark. (1997): Bacteriological culture of blood from critically ill neonatal calves. Can Vet J, 38, 95-100.
12. Gazioğlu A, Güvenç M (2015): Leptospirozisli danalarda oksidatif stres ve bazı biyokimyasal parametreler. F Ü Sağ Bil Vet Derg, 29, 45-48.
13. Ghanbarpour R, Oswald E (2009): Characteristics and virulence genes of Escherichia coli isolated from septicemic calves in southeast of Iran. Trop Anim Health Prod, 41, 1091-1099.
14. Halliwell B, Chirico S (1993): Lipid peroxidation: Its mechanism, measurement, and significance. Am J Clin Nutr, 57, 715-724.
15. Hassan N, Sheikh GN, Hussian SA ve ark. (2014): Variation in clinical findings associated with neonatal colibacillosis in lambs before and after treatment. Veterinary World, 7, 262-265.
16. Heffner JE, Repine JE (1989): Pulmonary strategies of antioxidant defense. Am Rev Respir Dis, 140, 531-554.
17. Huang WC, Juang SW, Liu IM ve ark. (1999): Changes of superoxide dismutase gene expression and activity in the brain of streptozotocin-induced diabetic rats. Neurosci Lett, 275, 25-28.
18. Janero DR (1990): Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med, 9, 515- 540.
19. Kapoor K, Basu S, Das BK ve ark. (2006): Lipid peroxidation and antioxidants in neonatal septicemia. J Trop Pediatr, 52, 372-375.
20. Kargın F, Fidancı UR (2001): Böbrek hastalıklı köpeklerde antioksidatif metabolizma. Türk Veterinerlik ve Hayvancılık Dergisi, 25, 607-613.
21. Kumar A, Pant P, Basu S ve ark. (2007): Oxidative stress in neonatal hyperbilirubinemia. J Trop Pediatr, 53, 69-71.
22. Kutsal A, Alpan O, Arpacık R (1990): İstatistik Uygulamalar. Bizim Büro Basımevi, Ankara.
23. Lykkesfeldt J, Svendsen O (2007): Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J, 173, 502-511.
24. Miller JK, Brzezinska-Slebodzinska E, Madsen FC (1993): Oxidative stress, antioxidants, and animal function. J Dairy Sci, 76, 2812-2823.
25. Morris WE, Venzano AJ, Elizondo A ve ark. (2011): Necrotic enteritis in young calves. J Vet Diagn Invest, 23, 254-259.
26. Narad SG, Gupta MH, Pakhmode SE (2013): Role of non enzymatic antioxidants in neonatal septicemia. Int J Cur Tr Res 2, 378-380.
27. Paglia DE, Valentine WN (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 70, 158-169.
28. Pekcan M, Fidancı UR, Yüceer B ve ark. (2013): Estimation of passive immunity in newborn calves with routine clinical chemistry measurements. Vet J Ankara Univ, 60, 85-88.
29. Sies H (1997): Physiological society symposium: Impaired endothelial and smooth muscle cell function in oxidative stress. Experimental Physiology, 82, 291-295.
30. Sordillo LM, Aitken SL (2009): Impact of oxidative stress on the health and immune function of dairy cattle. Vet Immunol Immunopathol, 128, 104-109.
31. Sun Y, Oberley LW, Li Y (1988): Simple for clinical assay of superoxide dismutase. Clin Chem, 34, 497-500.
32. Tüközkan N, Erdamar H, Seven I (2006): Measurement of total malondialdehyde in plasma and tissues by high- performanced liquid chromatography and thiobarbituric acid assay. Fırat Tıp Dergisi, 11, 88-92.
33. Yoshioka T, Kawada K, Shimada T ve ark. (1979): Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol, 135, 372-376. Geliş tarihi:12.04.2016 / Kabul tarihi: 09.08.2016