In vivo and in vitro assessment of ovarian echotexture through computer assisted real time ultrasonography in bitches

Abstract

In vivo and in vitro assessment of canine ovaries (n=72) were studied by computer assisted real time ultrasonography to analyse the correlation between the ovarian echotexture and serum progesterone (P4) concentration. Pre- and postoperative ovarian images were taken via B-mode ultrasonography, and analysed by an image analysis software package for total area of ovary (A), mean greyness value (MGV), heterogeneity (H), and minimum-maximum pixel value (PR). The ovaries were classified into follicular or luteal stage based upon the serum P4 concentration, vaginal cytology findings, and macroscopic inspection of ovaries postoperatively. The P4 was negatively (P<0.05) correlated with A, H and PR, whereas, it’s correlation with MGV was positive (P<0.05) in follicular stage. Also, parameters A, H and PR were higher (P<0.05) in follicular stage. In vitro parameters of MGV, H and PR were higher (P<0.05) than in vivo parameters in follicular stage. Meanwhile, in luteal stage ovaries, only in vitro MGV was higher than in vivo MGV. In this study, it was observed that the relationship between ovarian echotexture and oestrous stages could be presented numerically. Moreover, the differences between the in vivo and in vitro ovarian images were more prominent in bitches in the follicular stage

Keywords

• Bitch
• image analysis
• ovarian echotexture
• ultrasonography

Köpeklerde ovaryum ekotekstürünün bilgisayar destekli B - mod ultrasonografi ile in vivo ve in vitro incelenmesi

Abstract

Köpeklerde ovaryum ekotekstürü ile serum progesteron (P4) değerleri arasındaki ilişkinin belirlenmesi için köpek ovaryumları (n=72) in vivo ve in vitro olarak bilgisayar destekli real time ultrasonografi ile incelendi. Total ovaryum alanı (A), ortalama grilik değeri (MGV), heterojenite (H) ve minimum-maksimum piksel değerinin (PR) görüntü analiz yazılım sistemi ile analiz edilmesi için preoperatif ve postoperatif ovaryum B-mod ultrasonografi görüntüleri alındı. Ovaryumlar, serum P4 değerleri, vajinal sitoloji bulguları ve postoperatif makroskobik görünümlerine göre folliküler ve luteal evre olarak sınıflandırıldı. Folliküler evre grubunda P4 değeri A, H ve PR ile negatif korelasyon gösterirken (P<0.05), MGV ile pozitif korelasyon gösterdi (P<0.05). Yine folliküler evrede A, H ve PR değerleri önemli ölçüde yüksekti (P<0.05). Folliküler evrede in vitro görüntülerindeki MGV, H ve PR değerleri in vivo görüntülerdekinden daha yüksek bulundu (P<0.05). Ancak, luteal evredeki ovaryumlarda, sadece MGV in vitro görüntülerde daha yüksekti. Bu çalışmada, ovaryum ekotekstürü ile östrus dönemleri arasında sayısal olarak sunulabilen bir ilişki olduğu ve folliküler evredeki köpeklerde in vivo ve in vitro görüntüler arasında daha belirgin bir fark bulunduğu görüldü

Keywords

• Görüntü analizi
• köpek
• ovaryum ekotekstürü
• ultrasonografi

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