First isolation of Vibrio furnissii (emerging Vibrio) from mussels (Mediterranean mussel and bearded mussel) in Turkey

Abstract

Vibrio furnissii is an emerging pathogen that can cause acute gastroenteritis in humans. In this work, 652 bivalve mollusc samples (wedge shell-Donax trunculus, L.1758, oyster-Ostrea edulis, L.1758, cockle-Venus verrucosa, L.1758, clam-Tapes decussatus, L.1758, bearded mussel-Modiolus barbatus, L.1758, Mediterranean mussel-Mytilus galloprovincialis L.1819, striped venus-Chamelea gallina L.1753) sent to the laboratory from stations in Ayvalık and Balıkesir (Northern Aegean Region of Turkey) between 2007-2010 were researched with conventional microbiological methods and six isolates were identified as Vibrio furnisii. Isolates were later confirmed with PCR using primers specific to toxR gene. Isolation rate of V. furnisii from bivalve molluscs per years were 2007 (2%), 2008 (0.43%), 2009 (1.29%), 2010 (0%) and per shell fish species were mediterranean mussel (3.33%), bearded mussel (3.27%), oyster (3.27%), clam (0%), cockle (0%), wedge shell (0%), striped venus (0 %). In this work, V. furnissi was isolated from mussels in Turkey for the first time and attention was drawn zoonotic importance of the agent

Keywords

• Bearded mussel
• emerging Vibrio
• Mediterranean mussel
• Vibrio furnissii

Türkiye’deki midyelerden (Kara midye ve kıllı midye) ilk Vibrio furnissii (emerging Vibrio) izolasyonu

Abstract

Vibrio furnissii insanlarda akut gastroenteritise sebep olan ve önemi gittikçe artan bir patojendir. Bu çalışmada, 20072010 yılları arasında, Ayvalık ve Balıkesir’deki (Türkiye’nin Kuzey Ege bölgesi) istasyonlardan laboratuvara gönderilmiş 652 takım çift kabuklu yumuşakça (kum şırlanı-Donax trunculus, L. 1758, yassı istiridye-Ostrea edulis, L. 1758, kidonya-Venus verrucosa, L. 1758, akivades-Tapes decussatus, L. 1758, kıllı midye -Modiolus barbatus, L. 1758, kara midye - Mytilus galloprovincialis L. 1819, kum midyesi, Chamelea gallina L.1753) konvansiyonel mikrobiyolojik yöntemlerle araştırıldı ve çalışmada izole edilen altı izolatın V. furnissi olduğu tespit edildi. İzolatlar daha sonra toxR genine spesifik primerler kullanılarak PCR ile konfirme edildi. Çalışmada kullanılan çift kabuklu yumuşakçalardan V. furnissii izolasyon oranı yıl ve tür bazında 2007 (% 2), 2008 (% 0.43), 2009 (% 1.29), 2010 (% 0); kara midye (% 3.33), kıllı midye (% 3.27), istiridye (% 3.27), akivades (% 0), kidonya (% 0), kum şırlanı (% 0), kum midyesi (% 0) olarak tespit edildi. Bu çalışmada Türkiye’deki midyelerden ilk kez V. furnissi izole edilmiş olup, etkenin zoonotik önemi vurgulanmıştır

Keywords

• Emerging Vibrio
• kara midye
• kıllı midye
• Vibrio furnissii

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