The evaluation of various radioimmunoassay progesterone kits for different stage of reproductiveperiod in the cow, sheep and bitch

Abstract

The aim of this study was to compare blood serum progesterone levels by using different progesterone radioimmunoassay kits, in different stages of sexual cycle and pregnancy in the cow (n=25), sheep (n=25) and bitch (n=25). Progesterone levels in blood serum samples of the materials were measured by human progesterone RIA kit (Immunotech®), bovine, ovine and canine progesterone RIA kit (Immunotech®) and a validated human RIA kit. Measurements were performed according to instructions of the kits. As a validation, standart interval was extended and standart points were set to 0, 2.5, 5, 10, 20, 40, 80 and 100 ng/ml of the human kit. There was no statistically important difference between the mean progesterone values obtained by using different test kits (p>0.05). The results were correlated at the level of r=0.999 and r=1.000 for cows; r=0.997 and r=1.000 for sheeps and r= 0.994 and 0.998 for bitches. In conclusion, human RIA and validated human RIA kits can be use safely instead of species specific test kits for the blood progesterone mesurements in the cows, ewes and bitches

Keywords

• Domestic animals
• pregnancy gebelik
• progesterone kit radioimmunoassay
• cyclic periods

İnek, koyun ve köpeklerde değişik radioimmunoassay progesteron ölçüm kitlerinin reprodüktif sürecin farklı evrelerinde değerlendirilmesi

Abstract

Bu çalışmanın amacı, inek (n=25), koyun (n=25) ve dişi köpeklerin (n=25) farklı siklik evrelerinde, gebelik dönemlerinde ve dişi köpeklerin yalancı gebeliklerinde, kan serumunda progesteron düzeylerini türe spesifik (Immunotech®), insanlarda kullanılan (Immunotech®) ve bu çalışma için oluşturulan standart aralığı genişletilmiş radioimmunoassay kitleri (SAGK) ile ölçerek tanı yönünden uyum ve olası farklılıkları ortaya koymaktır. Ölçümlerde kullanılan beşeri kit ve türe spesifik kitler ile ölçümler üretici firmanın belirtmiş olduğu yönteme göre yapılmıştır. SAGK ile ölçüm yapabilmek için öncelikle standart aralığı genişletilmiş ve standart noktalar olarak 0, 2.5, 5, 10, 20, 40, 80 ve 100 ng/ml düzeyleri kullanılmıştır. Bu kit ile yapılan ölçümlerde takip edilen süreç, önceki iki kit ile aynı şekilde yürütülmüştür. Ölçümlerden elde edilen sonuçlar değerlendirildiğinde; tüm gruplardan her üç kit kullanılarak elde edilen sonuçlar arasında istatistiksel yönden önemli bir farklılığın olmadığı (p>0.05) ortaya konulmuştur. Kitlerden elde edilen sonuçların korelasyonu yapıldığında ise inekte r= 0.999 ve r=1.000; koyunda r=0.997 ve r=1.000 ve köpekte r=0.994 ve 0.998 düzeyinde bir ilişki bulunmuştur. Sonuç olarak, inek, koyun ve dişi köpeklerde progesteron ölçümü amacıyla türe spesifik kitler yerine, standart aralığı genişletilen veya beşeri kitlerin sağlıklı biçimde kullanılabileceği kanısına varılmıştır

Keywords

• Evcil hayvanlar
• gebelik
• progesteron kiti
• radioimmunoassay
• siklik dönemler

References

1. Baştan A, Alaçam E, Güven B, Fındık M, Erünal M (1998): Puerperal dönemdeki Holstein ineklerinde ultrasonografi ve kan hormon değerlerinin yardımıyla ovariumlardaki follikül dinamiğinin incelenmesi. Ankara Univ Vet Fak Derg, 45, 73-81.
2. Concannon PW, Hansel W (1977): Prostaglandin F2α induced luteolysis. Hypotermia, and abortions in beagle bitches. Prostaglandins, 13, 533-542.
3. Concannon PW, Hansel W, McEntee K (1977):Changes in LH, progesterone and sexual behavior associated with preovulatory luteinization in the bitch. Biol Reprod, 17, 604-615.
4. Concannon PW, McCann JP, Temple M (1989): Biology and endocrinology of ovulation, pregnancy and parturition in the dog. J Reprod Fertil Suppl, 39, 3-25.
5. Eckersall PD, Harvey MJA (1987): The use of a bovine plasma progesterone elısa kit to measure progesterone in equine, ovine and canine plasmas. Vet Rec, 120, 5-8.
6. Edquvist LE, Johansson EDB, Kasström H, Olsson SE, Richkind M(1975):Blood plasma levels of progesterone and estradiol in the dog during the oestrrus cycle and pregnancy. Acta Endocrinol, 78, 554-564.
7. Feldman EC, Olson RW (1996): Canine and Feline Endocrinology and Reproduction. WB Saunders, Philadelphia.
8. Goodman M(2001): Ovulation timing. Vet Clin North Am: Small Anim Pract, 31, 219-235.

9. Hafez ESE (1993): Hormones, growth factors and reproduction. 59-93. In: ESE Hafez (Ed), Reproduction in Farm Animal. Lea and Febiger, Philadelphia.
10. Hoffmann B, Busges F, Engel E and ark. (2004): Regulation of corpus luteum-function in the bitch. Reprod Dom Anim, 39, 232-240.
11. Hopkins SM (2003): Reproductive patterns of cattle. 395- 412. In: MH Pineda, MP Dooley (Ed), McDonald’s Veterinary Endocrinology and Reproduction. Iowa State Pres, Iowa.
12. Knickerbocker JJ, Drost M, Thatcher WW (1986): Endocrine patterns during the initiation of puberty, the estrous cycle, pregnancy and parturition in cattle. 117- 124. In: DA Morrow (Ed), Current Therapy in Theriogenology. WB Saunders, Philadelphia.
13. Montgomery ME, Leslie KE, Martin SW (1985): The sensitivity and spesifity of postbreeding plasma progesterone levels as a pregnancy test for dairy cows. Can J Comp Med, 49, 346-349.
14. Nachreiner RF (1986): Laboratory endocrine diagnostic procedures in theriogenology. 17-20. In: DA Morrow (Ed), Current Therapy in Theriogenology. WB Saunders, Philadelphia.
15. Olson PN, Bowen RA, Behrendt MD, Olson JD, Nett TM (1982): Concentrations of reproductive hormones in canine serum throught late anestrus, proestrus and estrus. Biol Reprod, 27, 1196-1206.
16. Özyurtlu N, Ay SS, Alaçam E (2006): Evaluation of vaginal smears and progesterone and relaxin levels in pregnant and overt and covert pseudopregnant bitches. Turk J Vet Anim Sci, 30, 465-470.
17. Phemister RD, Holst PA, Spano JS, Hopwood ML (1973):Time of ovulation in the beagle bitch. Biol Reprod, 8, 74-82.
18. Pineda MH (2003): Reproductive patterns of dogs. 475- 504. In: MH Pineda, MP Dooley (Ed), McDonald’s, Veterinary Endocrinology and Reproduction. Iowa State Pres, Iowa.
19. Post K, Evans LE, Jöchle W (1988): Effects of prolactin suppression with cabergoline on the pregnancy of the bitch. Theriogenology, 29, 1233-1243.
20. Reimers TJ(2003): Introduction. 1-15. In: MH Pineda, MP Dooley (Ed), McDonald’s Veterinary Endocrinology and Reproduction. Iowa State Pres, Iowa.
21. Shemesh M, Ayalon N, Linder HR (1973): Early pregnancy diagnosis based upon plasma progesterone levels in the cow and ewe. J Anim Sci, 36, 726-729.
22. Smith MS, McDonald LE(1974):Serum levels of luteinizing hormone and progesterone during the estrus cycle, pseudopregnancy and pregnancy in the dog. Endo, 94, 404-412.
23. Stabenfeldt GH, Edqvist LE, Kindahl H, Gustafson B, Bane A physiological findings for reproductive efficiencey in cows, mares, sows and ewes. JAVMA, 172, 667-672.
24. Stellflug JN, Weems YS, Weems CW (1997): Clinical reproductive physiology of ewes. 594-598. In: RS Youngquist (Ed), Current Therapy in Large Animal Theriogenology. WB Saunders, Philadelphia.
25. Stevenson JS(1997): Clinical reproductive physiology of the cow. 257-267. In: RS Youngquist (Ed), Current Therapy in Large Animal Theriogenology. WB Saunders, Philadelphia.
26. Sulu N, Özsar S, Güven B, Bağcı C (1993): Sakız koyunlarında progesteron ve östron sülfat düzeyleri. Doğa- Tr J Vet Anim Sci, 17, 9-17.
27. Weillenmann R, Arnold S, Dobeli M, Rüsch P, Zerobin K(1993): Östradiol und progesteronkonzentrationen im plasma von nicht trächtigen hündinnen während des sexualzyklus. Schweiz Arch Tierheilk, 135, 51-57.
28. Wright PJ (1990): Appication of vaginal cytology and plasma progesterone determinations to the management of reproduction in the bitch. J Small Anim Pract, 31, 335- 340.
29. Wright PJ (1991): Practical aspects of the estimation of the time of ovulation and of insemination in the bitch. Aust Vet J, 68, 10-13.