Research Article

Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Volume: 67 Number: 3 June 3, 2020
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Ankara Üniversitesi Veteriner Fakültesi Dergisi
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Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Abstract

The therapeutic potential of the adipose tissue is also supported by a source of mesenchymal stem cells (MSCs), endothelial progenitor cells, mast cells, T- lymphocyte, B lymphocyte, and adipose-resident macrophages with repairing and regenerative ability. The purpose of this study was to compare adipose tissue derived mesenchymal stem cells (ADSCs) and stromal vascular fraction cells (SVF cells) in terms of the usage of non-enzymatic isolation, detection of cell adhesion, fibroblastoid cell formation, properties of cell population, cell culture duration until 3rd passages under in-vitro culture condition. We used 3 months old, 4 male Sprague Dawley rats (mean of live weight about 250 g) to examined the variation of the cell population, cell properties, phases of cellular formation, cell culturing time, subculturing duration, differences in confluency between SVF cells and ADSCs. In in-vitro expansion, ADSCs displayed higher rate of adhesiveness, homogenous cell population, faster proliferation and formation of fibroblast like cells compared to SVF cells. As a result, we showed that ADSCs have better adhesive ability, higher proliferative capacity in all of the 3 passages and require shorter time to reach confluency compared with SVF cells in vitro. These findings may contribute to future studies that deal with isolation and selection of stem cells from various tissues, as well as design clinical trials based on ADSCs and SVF cells.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Surgery

Journal Section

Research Article

Authors

Publication Date

June 3, 2020

Submission Date

December 16, 2019

Acceptance Date

February 12, 2020

Published in Issue

Year 2020 Volume: 67 Number: 3

DOI

https://doi.org/10.33988/auvfd.660139

IZ

https://izlik.org/JA45ZC79XC

Cite

RIS / Bibtex
APA
Çerçi, E., & Erdost, H. (2020). Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 67(3), 295-301. https://doi.org/10.33988/auvfd.660139
AMA
1.Çerçi E, Erdost H. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. 2020;67(3):295-301. doi:10.33988/auvfd.660139
Chicago
Çerçi, Ece, and Hatice Erdost. 2020. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 (3): 295-301. https://doi.org/10.33988/auvfd.660139.
EndNote
Çerçi E, Erdost H (June 1, 2020) Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67 3 295–301.
IEEE
[1]E. Çerçi and H. Erdost, “Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells”, Ankara Univ Vet Fak Derg, vol. 67, no. 3, pp. 295–301, June 2020, doi: 10.33988/auvfd.660139.
ISNAD
Çerçi, Ece - Erdost, Hatice. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 67/3 (June 1, 2020): 295-301. https://doi.org/10.33988/auvfd.660139.
JAMA
1.Çerçi E, Erdost H. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. 2020;67:295–301.
MLA
Çerçi, Ece, and Hatice Erdost. “Non Enzymatic Isolation of Adipose Tissue and Stromal Vascular Fraction Derived Cells”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 67, no. 3, June 2020, pp. 295-01, doi:10.33988/auvfd.660139.
Vancouver
1.Ece Çerçi, Hatice Erdost. Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells. Ankara Univ Vet Fak Derg. 2020 Jun. 1;67(3):295-301. doi:10.33988/auvfd.660139

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