Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis

Abstract

Quorum Sensing is a special system between bacteria to communicate with the signal molecules. Propolis is a resin collected from plants by honey bees. The present study examined anti-quorum sensing and antimicrobial activities of water and ethanol extracts of propolis. The antimicrobial activities of the propolis extracts were studied against Escherichia coli ATCC 25922 Gram (-), Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 Gram (+), Candida albicans ATCC 10239 (yeast) by the well diffusion method. Anti-quorum sensing activity, violacein inhibition activity, and anti-swarming activity were studied using Chromobacterium violaceum CV026 and C. violaceum ATCC 112472, and Pseudomonas aeruginosa (PA01) respectively. Both propolis extracts were detected antibacterial activity against Gram (+) bacteria and Candida albicans. However, antibacterial activity against Gram (-) bacteria were not observed. While the antimicrobial activity of water extract propolis was determined more effectively than ethanol extract, anti-quorum sensing and anti-swarming activities less effective. It was also detected that both extracts display violacein inhibition. It was revealed that the antimicrobial, anti-quorum sensing, anti-swarming activity depended on the dose of propolis extracts. Thirty-six phenolic compounds were analysed of propolis extract by HPLC-DAD. The amount and presence of phenolic compounds in the ethanol extract were more than the water extract apart from benzoic acid, cinnamic acid, syringic acid, (±) epigallocatechin, vitexin, rutin, and trans-chalcone. Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti- quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.

Keywords

• antimicrobial activity
• anti-quorum sensing activity
• anti-swarming activity
• violacein inhibition activity
• Propolis

References

1. Alvarez MV, Moreira MR, Ponce A (2012): Antiquorum Sensing and Antimicrobial Activity of Natural Agents with Potential Use in Food. J Food Saf, 32, 379-387.
2. Astani A, Zimmermann S, Hassan E, et al (2013): Antimicrobial activity of propolis special extract GH 2002 against multidrug-resistant clinical isolates. Die Pharmazie, 68, 695-701.
3. Banskota AH, Tezuka Y, Kadota S (2001): Recent progress in pharmacological research of propolis. Phytother Res, 15, 561-571.
4. Boonsai P, Phuwapraisirisan P, Chanchao C (2014): Antibacterial activity of a cardanol from Thai Apis mellifera propolis. Int J Med. Sci, 11, 327.
5. Bulman Z, Hudson A, Savka MA (2011). Propolis, a product of the bee hive, has an antagonistic affect on quorum-sensing regulated bioluminescence, gene transcription and motility. The FASEB Journal, 25, 948.
6. Bulman Z, Le P, Hudson AO, et al (2011): A novel property of propolis (bee glue): anti-pathogenic activity by inhibition of N-acyl-homoserine lactone mediated signaling in bacteria. J Ethnopharmacol, 138, 788-797.
7. De Castro S (2001): Propolis: biological and pharmacological activities. Therapeutic uses of this bee-product. Annu Rev Biomed, 3, 49-83.
8. De Marco S, Piccioni M, Pagiotti R, et al (2017): Antibiofilm and antioxidant activity of propolis and bud poplar resins versus Pseudomonas aeruginosa. eCAM, 2017.

9. Djais AA, Putri N, Jemmy J, et al (2019): Effect of Propolis on Streptococcus mutans Biofilm Formation. Pesqui Bras Odontopediatria Clin Integr, 19, 5221.
10. Dong Y-H, Zhang L-H (2005): Quorum sensing and quorum-quenching enzymes. J Microbiol , 43, 101-109.
11. Gemiarto AT, Ninyio NN, Lee SW, et al (2015): Isoprenyl caffeate, a major compound in manuka propolis, is a quorum-sensing inhibitor in Chromobacterium violaceum. Anton Leeuw Int J G, 108, 491-504.
12. Gonsales G, Orsi R, Fernandes Júnior A, et al (2006): Antibacterial activity of propolis collected in different regions of Brazil. J Venom Anim Toxins Incl Trop Dis, 12, 276-284.
13. Kasote D, Ahmad A, Chen W, et al (2015): HPTLC-MS as an efficient hyphenated technique for the rapid identification of antimicrobial compounds from propolis. Phytochemistry Letters, 11, 326-331.
14. Koh KH, Tham F-Y (2011): Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. J Microbiol Immunol Infect, 44, 144-148.
15. Kubiliene L, Laugaliene V, Pavilonis A, et al (2015): Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complement Altern Med, 15, 156.
16. Lamberte LE, Cabrera EC, Rivera WL (2011): Activity of the Ethanolic Extract of Propolis (EEP) as a Potential Inhibitor of Quorum Sensing-Mediated Pigment Production in Chromobacterium violaceum and Virulence Factor Production in Pseudomonas aeruginosa. Philipp Agric Sci, 94, 14-22.
17. Lotfy M (2006): Biological activity of bee propolis in health and disease. Asian Pac J Cancer Prev, 7, 22-31.
18. Magaldi S, Mata-Essayag S, De Capriles CH, et al (2004): Well diffusion for antifungal susceptibility testing. Int J Infect Dis, 8, 39-45.
19. Majiene D, Trumbeckaite S, Pavilonis A, et al (2007): Antifungal and antibacterial activity of propolis. Curr Nutr Food Sci, 3, 304-308
20. McLean RJ, Pierson III LS, Fuqua C (2004): A simple screening protocol for the identification of quorum signal antagonists. J Microbiol Methods, 58, 351-360.
21. Noori A, Al-Ghamdi A, Ansari MJ, et al (2012): Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci, 9, 793.
22. Nyandwi R, Kılıç AS, Çelik M, et al (2019): Determination and Quantification of Gallic Acid in Raw Propolis by High-performance Liquid Chromatography–Diode Array Detector in Burundi. East Afr J Sci, 1, 43-48.
23. Packiavathy IASV, Agilandeswari P, Musthafa KS, et al (2012): Antibiofilm and quorum sensing inhibitory potential of Cuminum cyminum and its secondary metabolite methyl eugenol against Gram negative bacterial pathogens. Food Res Int, 45, 85-92.
24. Pobiega K, Kraśniewska K, Derewiaka D, et al (2019): Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods. J Food Sci Technol, 56, 5386-5395.
25. Przybyłek I, Karpiński TM (2019): Antibacterial properties of propolis. Molecules, 24, 2047.
26. Rasmussen TB, Givskov M (2006): Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol, 296, 149-161.
27. Santos HCD, Vieira DS, Yamamoto SM, et al (2019): Antimicrobial activity of propolis extract fractions against Staphylococcus spp. isolated from goat mastitis. Pesqui Vet Bras, 39, 954-960.
28. Sariyev R, Çayci M, Oruç HH (2019): Azerbaycan Propolislerinin Fenolik Madde İçerikleri ve Kalite Açısından Değerlendirilmesi. J Res Vet Med, 38, 1-6.
29. Savka MA, Dailey L, Popova M, et al (2015): Chemical composition and disruption of quorum sensing signaling in geographically diverse United States propolis. eCAM, 2015.
30. Sforcin JM, Bankova V (2011): Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133, 253-260.
31. Silva JC, Rodrigues S, Feás X, et al (2012): Antimicrobial activity, phenolic profile and role in the inflammation of propolis. Food ChemToxicol, 50, 1790-1795.
32. Song X, Xia Y-X, He Z-D, et al (2018): A review of natural products with anti-biofilm activity. Curr Org Chem, 22, 789-817.
33. Sorucu A, Oruç HH (2019): Determination of biologically active phenolic compounds in propolis by LC–MS/MS according to seasons and altitudes. J Food Meas Charac, 13, 2461-2469.
34. Volpi N, Bergonzini G (2006): Analysis of flavonoids from propolis by on-line HPLC–electrospray mass spectrometry. J Pharm Biomed Anal, 42, 354-361
35. Wayne P (2011): Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing.