Türkiye kıyılarından Avrupa deniz levreği Dicentrarchus labrax (L.)’ı enfekte eden Diplectanum aequans (Diplectanidae: Monogenea)’in morfolojik ve moleküler karakterizasyonunun bütünleştirici bir çalışması

Abstract

Monogeneanlar deniz balıklarının yaygın ektoparazitik yassı kurtlarıdır. Diplectanid monogenean Diplectanum aequans (Wegener, 1857) (Diplectanidae) dünya çapında doğal ve kültüre edilen Avrupa deniz levreği Dicentrarchus labrax (Linnaeus, 1758) türünün en önemli ektoparazitlerden biridir. Bu parazit türü Avrupa deniz levreklerinin solungaçlarında ciddi patolojik hasarlara ve enfekte levreklerde ölüme neden olur. Halen Türkiye kıyılarından D. aequans'ın moleküler verileri hakkında bilgi bulunmamaktadır. Bu çalışmada Türkiye'nin Karadeniz (FAO 37.4.2) ve Ege Denizi (FAO 37.1.3) kıyılarındaki doğal ve kültüre edilen D. labrax (L.)'dan diplectanid monogeneanlar toplandı, morfolojik olarak identifiye edildi ve 28S rRNA ile 18S rRNA parçalarının DNA dizilenmesiyle genetik olarak karakterize edildi. Toplanan diplectanidler ayrıntılı morfolojik özelliklerine ve 28S rRNA ile 18S rRNA genlerinin dizi karakterizasyonuna bağlı olarak açık bir şekilde D. aequans olarak teşhis edildi. Diplectanum aequans'ın enfeksiyon oranı ve ortalama yoğunluğu sırasıyla %100 ve 15,1 idi. Bu çalışma Türkiye kıyılarından örneklenen D. labrax'tan izole edilen D. aequans'ın moleküler (28S ve 18S rRNA) kanıtlarına ilişkin ilk bilgileri sunmaktadır. Karadeniz (FAO 37.4.2) ve Ege Denizinden (FAO 37.1.3) izole edilen D. aequans için yeni 18S ve 28S rRNA dizileri genetik olarak karakterize edilmiştir. Diplectanum aequans'ın 28S ve 18S rRNA dizileri Karadeniz ve Akdeniz'deki Diplectanidae türlerinin filogenetik pozisyonlarını çözmek için kullanılabilir.

Keywords

• Diplectanidae
• DNA dizi analizi
• Filogenetik analiz
• 18S rRNA
• 28S rRNA

An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (Linnaeus, 1758) from Turkish coasts

Abstract

The diplectanid monogenean Diplectanum aequans (D. aequans) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax (Dic. labrax) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.

Keywords

• Diplectanidae
• DNA sequencing
• Phylogenetic analysis
• 18S rRNA
• 28S rRNA

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