BOTULİSMUSLU İNEKLERDE SERUM KOLİNESTERAZ VE AMİLOİD A SEVİYELERİNİN DEĞERLENDİRİLMESİ

Abstract

GİRİŞ ve AMAÇ: Bu çalışmada botulismuslu ve sağlıklı ineklerde serum kolinesteraz, amiloid A, albumin, total protein, total bilirubin, üre, GGT ve SDH araştırıldı.

YÖNTEM ve GEREÇLER: Çalışma ve kontrol grubunu gebe ve kuru dönemde iki ile beş yaşları arasında değişen 10 adet botulismuslu ve 6 adet sağlıklı holştayn inek oluşturdu. Parametrelerin serum düzeyleri Nefelometre BNII ve otoanalizör kullanılarak ölçüldü. Botilusmuslu hayvanların kan serumlarından ve silajdan fare inokulasyon testi yapıldı.

BULGULAR: Botulismuslu ineklerin kan serumlarında ve silajda Clostridium botulinum tip C ve tip D toksini tespit edildi. Serum, kolinesteraz, amiloid A, albumin, total protein, total bilirubin ve SDH botulismuslu ve sağlıklı ineklerde önemli bir değişiklik göstermezken botulismuslu ineklerde üre ve GGT düşük çıktı.

TARTIŞMA ve SONUÇ: Botulismuslu ineklerde kolinesteraz ve formlarının araştırılması botulismusun fizyopatolojisine ve prognozuna faydalı olabileceği sonucuna varıldı.

Keywords

• Botulismus,inek,kolinesteraz,serum amiloid A,toksin

EVALUATION OF SERUM CHOLINESTERASE AND AMYLOID A LEVELS IN COWS WITH BOTULISM

Abstract

INTRODUCTION: The aim of this study was to determine serum cholinesterase, amyloid A, albumin, total protein, total bilirubin, urea, GGT and SDH in cows with or without botulism.

METHODS: The study included 10 holstein cows aged two to five years as the botulism group and control group consisting of 6 healthy cows. Serum concentration of all the parameters were measured using an autoanalyzer and Nefelometre equipment BNII. Mice inoculation test was performed in silage and blood that cows with botulism.

RESULTS: Clostridium botulinum type C and D toxins were determined in silage and blood that cows with botulism. Serum cholinesterase, amyloid A, albumin, total protein, total bilirubin and SDH did not differ significantly between two groups. Serum showed that in the botulism group urea and GGT were lower than in the control group.

DISCUSSION AND CONCLUSION: Serum cholinesterase and forms researched that may prove beneficial physiopathology and prognosis of botulism.

Keywords

• Botulism,cow,cholinesterase,serum amyloid A

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