80 GÜNLÜK YABAN DOMUZU FÖTÜSLERİNDE ÖN VE ARKA EXTREMİTELERİN KEMİKLEŞME VE GELİŞİMLERİ

Abstract

GİRİŞ ve AMAÇ:: Normal kemik gelişim verileri kemik gelişim çalışmalarını yorumalamak ve açıklamak için vazgeçilmez parametrelerdir. Bu çalışmada 80 günlük yaban domuzu fetüslerine ait 32 ekstremite kullanılmış, extremitelerinin normal kemik gelişiminin ortaya çıkartılması amaçlanmıştır. YÖNTEM ve GEREÇLER: Materyaller % 5’lik formaldehit solusyonunda tespit edildikten ve % 95’lik etanolde bekletildikten sonra saf aseton ile muamele edildi. Materyaller alizarin red alcian blue kombinasyonu ile boyandı. Bu boyama tekniği kıkırdak dokuları maviye, kemik dokuları ise kırmızıya boyadı. Bu teknik gelişen kıkırdak dokuları ve kemiklerdeki erken gelişen kemikleşme merkezlerinin lokalizasyonunu gösterdi. Stereomikroskop altında kıkırdaklaşma ve kemikleşme zamanları dikkatle izlendi.

BULGULAR: Büyük kemiklerin herbirinin bir adet primer kemikleşme merkezine (POC) sahip olduğu gözlendi. Ossa carpi, ossa tarsi ve patella dışındaki tüm kemiklerde gövdedeki kemikleşmenin görüldüğü belirlendi. Görülen kıkırdak modellerin gelişmesini tamamlamış olanlara benzediği saptandı.

TARTIŞMA ve SONUÇ: Sadece radius’a ait bir örnekte distal kısımda sekonder kemikleşme merkezi (SOC) belirlendi.

Keywords

• Fötüs,kemik,kemikleşme,yaban domuzu

OSSIFICATION CENTERS IN THE BONE DRAFTS OF THE FORE- AND HIND- LIMBS OF 80 DAYS OLD

Abstract

OBJECTIVE: Having information about the normal skeletogenous stages is essential in order to comment on experimental bone development studies. With this regard, we aimed at revealing the normal ossification status of the 32 extremities of the 80 days old wild pig fetuses.

METHODS: The materials were fixed in 5% formaldehyde solution and 95% ethanol, followed by pure acetone incubation. Then, samples were stained with an alcian blue-alizarin red combination. As a result of the staining procedure, the cartilaginous tissue was stained in blue and the osseous tissue was stained in red. By using this method, it is possible to display the development of the cartilaginous components and localization of the early centers of the ossification areas in the bones. Chondrification and ossification of the bones were observed attentively under a stereo microscopy.

RESULTS: Each big bone has only one primary ossification body center (POC). The ossification centers in all bones have been shown whereas there are no ossification centers in the carpal, tarsal bones and patella. The extremities with the blue stained cartilaginous and red osseous tissues have resembled to the gross structural shapes of the mature stages.

DISCUSSION AND CONCLUSION: Only one secondary ossification center (SOC) was observed in the distal part of radius of one specimen.

Keywords

• Bone,fetal,ossification

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