Histopathological Examination of the Effects of Tocilizumab and Dexamethasone on the Liver in Rats of Oleic Acid induced Acute Lung Injury

Abstract

Objective: This study, it was aimed to examine the effects of Tocilizumab and Dexamethasone on the liver in rats in which the acute lung injury model was created with Oleic acid. Materials and Methods: Thirty-six male rats were used as study material and were divided into six groups as Control, OA, TCZ and DEX. All groups except the control group received OA intravenously and after OA injections other compounds were administered intravenously (DEX 0.1 mg/kg and DEX 10 mg/kg, TCZ 2 mg/kg and TCZ 4 mg/kg). Results: It was determined that hydropic degeneration increased in hepatocytes in OA+ DEX 10 group rats compared to other groups (p<0.05). Vacuole formation in hepatocytes in OA+TCZ 2 and 4 groups was found to be similar to the control group (p<0.05). In addition, necrosis in hepatocytes and narrowing of sinusoids were detected more severely in OA+DEX (0.1 and 10 mg/kg) groups. Conclusion: As a result, it was determined that high dose Dexamethasone had hepatotoxic effect. It is thought that liver function tests should be performed and evaluated to determine the hepatotoxic effects of drugs to be used in the treatment of patients with COVID/19 ARDS.

Keywords

• ARDS
• Tocilizumab
• Dexamethasone
• Liver
• Rat

Project Number

KÜ-BAP01/2019-12

Oleik Aside Bağlı Akut Akciğer Hasarı Modeli Oluşturulan Ratlarda Tocilizumab ve Deksametazon'un Karaciğer Üzerine Etkilerinin Histopatolojik Olarak İncelenmesi

Abstract

Amaç: Bu çalışmada, oleik asit ile akut akciğer hasarı modeli oluşturulan ratlarda Tocilizumab ve Deksametazon'un karaciğer üzerindeki etkilerinin incelenmesi hedeflenmiştir. Gereç ve Yöntem: Çalışma materyalini otuz altı erkek rat oluşturdu ve CN, OA, TCZ ve DEX olmak üzere altı gruba ayrıldı. Kontrol grubu dışındaki tüm gruplar intravenöz olarak OA verildi. OA enjeksiyonlarından sonra diğer bileşikler intravenöz olarak uygulandı (DEX 0.1 mg/kg ve DEX 10 mg/kg, TCZ 2 mg/kg ve TCZ 4 mg/kg). Bulgular: OA+DEX 10 grubu sıçanlarda hepatositlerde hidropik dejenerasyonun diğer gruplara göre arttığı belirlendi (p<0.05). OA+TCZ 2 ve 4 gruplarında hepatositlerde vakuol oluşumu kontrol grubuna benzer bulundu (p<0.05). Ayrıca OA+DEX (0,1 ve 10 mg/kg) gruplarında hepatositlerde nekroz ve sinüzoidlerde daralma daha şiddetli olduğu görüldü. Sonuç: Yüksek doz deksametazonun hepatotoksik etki gösterdiği belirlendi. COVID-19/ARDS hastalarının tedavisinde kullanılacak ilaçların hepatotoksik etkilerinin belirlenmesi için karaciğer fonksiyon testlerinin yapılması ve değerlendirilmesi gerektiği düşünülmektedir.

Keywords

• ARDS
• Tocilizumab
• Deksametazon
• Karaciğer

Supporting Institution

Kastamonu Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

KÜ-BAP01/2019-12

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