Determination of the Pharmacokinetics of Florfenikol in Turbot (Psetta maxima) Fish Grown in Black Sea Water

Yıl 2022, Cilt: 11 Sayı: Supplement 1 - Veteriner Farmakoloji Kongresi Özel Sayısı, 15 - 21, 01.12.2022

Erkan Taçbaş Emine Baydan Mustafa Türe İlyas Kutlu Cemil Altuntaş Gözde Yücel Tenekeci Farah Gönül Aydın Emre Arslanbaş

https://doi.org/10.53424/balikesirsbd.1160570

Öz

Objective: In this study, it was aimed to determine the pharmacokinetics of florfenicol (FF) in a single dose (10 mg kg-1) in Black Sea Turbot (Psetta maxima). Materials and Methods: The study was carried out in closed system pools within the Trabzon Fisheries Central Research Institute in Black Sea water (temperature was 11±1ºC, the salinity rate was 0.18 %, oxygen did not fall below 10.02 mg ml-1). Experiments were performed on 65 turbot fish (42±2 g) including validation (5 specimens) and drug trials (60 specimens). Turbot fish were divided into two groups as FF administered intramuscularly and gavage. Determination of plasma FF levels was performed on a high pressure liquid chromatography device (HPLC). Pharmacokinetic analyzes were performed using the win-nonlin pharmacokinetic program according to the non-compartmental model. Results: The results of the validation studies were determined as 96.19, 85.81±0.026, 0.0039 and 0.012 based on Relative Standard Deviation (RSD) %, mean recovery %, LOD and LOQ in ppm, respectively. Tmax, Cmax, Tlast, Clast, AUClast, Vz_F_obs and Cl_F_obs values of intramuscular and gavage FF administration groups are respectively; 1 and 6 hour; 6.60 and 5.17 mg L-1; 48 and 48 hour; 2.02 and 1.48 mg L-1; 145.39 and 101.76 hour mg L-1; 2.54 and 3.64 L kg-1; 0.033 and 0.045 L kg h-1. Conclusion: The results of the study showed that the pharmacokinetics of FF may vary depending on the fish species and growing conditions, such as water temperature, salinity.

Anahtar Kelimeler

Pharmacokinetics, Florfenicol, Turbot, Black Sea, Intramuscular, Intragastric (gavage).

Proje Numarası

TAGEM / HSGYAD/15/A11/P03/65

Karadeniz Suyunda Yetişen Kalkan (Psetta maxima) Balıklarında Florfenikol’ün Farmakokinetiğinin Belirlenmesi

Öz

Amaç: Bu araştırmada Karadeniz Kalkan Balığında (Psetta maxima) tek doz (10 mg kg-1) florfenikolün (FF) farmakokinetiğinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çalışma Trabzon Su Ürünleri Merkez Araştırma Enstitüsü Müdürlüğü bünyesinde bulunan kapalı sistem havuzlarda Karadeniz suyunda (sıcaklık 11±1ºC, tuzluluk oranı %0.18, oksijen miktarı 10.02 mg ml-1 altına düşürülmedi) gerçekleştirildi. Deneyler validasyon (5 adet) ve ilaç denemeleri (60 adet) dahil 65 adet kalkan balığında (42±2 gr) gerçekleştirildi. Kalkan balıkları kas içi ve gavaj yoluyla FF uygulanan gruplar olmak üzere iki gruba ayrıldı. Plazma FF düzeylerinin belirlenmesi yüksek basınçlı sıvı kromatografisi cihazında (HPLC) gerçekleştirildi. Farmakokinetik analizler kompartımanlı olmayan modele göre win-nonlin farmakokinetik program kullanılarak yapıldı. Bulgular: Validasyon çalışma sonuçları Göreceli standart sapma (RSD) (%), ortalama geri kazanım (%), LOD ve LOQ (ppm) değerleri üzerinden sırasıyla, %96.19, %85.81±0.026, 0.0039 ve 0.012 ppm olarak belirlendi. Kas içi ve gavaj yoluyla FF uygulama gruplarının Tmax, Cmax, Tlast, Clast, AUClast, Vz_F_obs, Cl_F_obs değerleri sırasıyla 1 ve 6 saat; 6.60 ve 5.17 mg L-1; 48 ve 48 saat; 2.02 ve 1.48 mg L-1; 145.39 ve 101.76 saat mg L-1; 2.54 ve 3.64 L kg-1; 0.033 ve 0.045 L kg saat-1 olarak belirlendi. Sonuç: Araştırma sonuçları FF farmakokinetiğinin balık türü ve yetiştirme koşulları ile ilgili su sıcaklığı, tuzluluk oranı gibi faktörlere bağlı olarak değişebileceğini göstermiştir.

Anahtar Kelimeler

Farmakokinetik, Florfenikol, Kalkan, Karadeniz, Kas içi, Mide içi (gavaj).

Destekleyen Kurum

Tarım ve Orman Bakanlığı, Tarımsal Araştırma ve Politikalar Genel Müdürlüğü (TAGEM / HSGYAD/15/A11/P03/65).

Proje Numarası

TAGEM / HSGYAD/15/A11/P03/65

Teşekkür

Farmakokinetik analizlere katkılarından dolayı Cengiz GÖKBULUT’a, çalışmamız için ortam ve destek sağlaya Trabzon Merkez Su Ürünleri Araştırma Enstitüsü yönetimine ve personeline teşekkür ederiz.

Kaynakça

• Al Shoyaib, A., Archie, S.R., & Karamyan, V.T. (2020). Intraperitoneal route of drug administration: Should it be used in experimental animal studies? Pharmaceutical research, 37(1), 1-17. https://doi.org/10.1007/s11095-019-2745-x.
• Anadón, A., Martínez, M. A., Martínez, M., Ríos, A., Caballero, V., Ares, I., & Martínez- Larrañaga, M. R. (2008). Plasma and tissue depletion of florfenicol and florfenicol-amine in chickens. Journal of Agricultural and Food Chemistry, 56(22), 11049-11056. https://doi.org/10.1021/jf802138y.
• Aydın, İ., & Şahin, T. (2011). Reproductive performance of turbot (Psetta maxima) in the southeastern Black Sea. Turkish Journal of Zoology, 35(1), 109-113. https://doi.org/10.3906/zoo-0905-26.
• Aydın, İ., Polat, H., & Sahin, T. (2019). Reproductive Performance of Wild and Hatchery-Reared Black Sea Turbot, Psetta maxima, in the Southern Black Sea Coast. Turkish Journal of Fisheries and Aquatic Sciences, 20(5), 351-357. http://doi.org/10.4194/1303-2712-v20_5_03.
• Aydın, İ., Polat, H., Küçük, E., & Özdemir, M. D. (2020). Turbot and flounder aquaculture. In: Marine Aquaculture in Turkey: Advancements and Management. Turkish Marine Research Foundation (TUDAV). Eds: Çoban, D., Demircan, M.D., Tosun, D.D. Publication No: 59, İstanbul, Turkey.
• Caruso, F., Pierraccini, J., Bourdenet, D., & Massa, F. (2018). Technical Training on Turbot Farming and Restocking in Trabzon, Turkey. FAO Aquaculture Newsletter No. 59.
• Cerveny, D., Fick, J., Klaminder, J., McCallum, E.S., Bertram, M.G., Castillo, N.A., & Brodin, T. (2021). Water temperature affects the biotransformation and accumulation of a psychoactive pharmaceutical and its metabolite in aquatic organisms. Environment International, 155, 106705. https://doi.org/10.1016/j.envint.2021.106705.
• Clinical and Laboratory Standards Institute (CLSI). (2022). M100 Performance Standards for Antimicrobial Susceptibility Testing. 28th Edition, ISBN 978-1-68440-134-5, USA.
• Eaton, K.D., & Lyman, G.H. (2018). Dosing of anticancer agents in adults. UpToDate, https://www.uptodate.com/contents/dosing-of-anticancer-agents-in-adults (accessed on 25 December, 2018).
• Ho, S-P, Hsu, T-Y, Che, M-H, & Wang W-S. (2000). Antibacterial effect of chloramphenicol, thiamphenicol and florfenicol against aquatic animal bacteria. The Journal of Veterinary Medical Science, 62(5), 479-485. http://doi.org/10.1292/jvms.62.479.
• Kalayci, G., Incoglu, S., & Ozkan, B. (2006). First isolation of viral haemorrhagic septicaemia (VHS) virus from turbot (Scophthalmus maximus) cultured in the Trabzon coastal area of the Black Sea in Turkey. Bulletin of the European Association of Fish Pathologists, 26(4), 157.
• Kogiannou, D., Nikoloudaki, C., Katharios, P., Triga, A., & Rigos, G. (2020). Evaluation of absorption and depletion of florfenicol in European seabass Dicentrarchus labrax. Veterinary Medicine and Science. 7(3), 987-997. http://doi.org/10.1002/vms3.415.
• Lin, Y., Yang, J., Wu, Z., Zhang, Q., Wang, S., Hao, J., Ouyang, L., & Li, A. (2022). Establishment of Epidemiological Resistance Cut-Off Values of Aquatic Aeromonas to Eight Antimicrobial Agents. Microorganisms, 10, 776. https://doi.org/10.3390/microorganisms10040776.
• Lis, M., Szczypka, M., Suszko, A., Świtała, M., & Obmińska-Mrukowicz, B. (2011). The effects of florfenicol on lymphocyte subsets and humoral immune response in mice. Polish Journal of Veterinary Sciences, 14(2), 191-198. http://doi.org/10.2478/v10181-011-0029-4.
• Ocenda, V-R de, Almeida-Prieto, S., Luzardo-Alvarez, A., Barja, J. L., Otero-Espinar, F.J., & Blanco-Mendez, J. (2017). Pharmacokinetic model of florfenicol in turbot (Scophthalmus maximus): Establishment of optimal dosage and administration in medicated feed. Journal of Fish Diseases. 40(3), 411-424. http://doi.org/10.1111/jfd.12525.
• Öztürk, R. Ç., & Altınok, İ. (2014). Bacterial and viral fish diseases in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 14, 275-297.
• Pourmolaie, B., Eshraghi, H. R., Haghighi, M., Mortazavi, S. A., & Rohani, M. S. (2018). Pharmacokinetics of florfenicol by gavage feeding or medicated feed in rainbow trout (Oncorhynchus mykiss). Journal of Aquaculture & Marine Biology, 7(1), 44-46.
• Samsun, N., Yiğit, M., & Çolak, S. Ö. (2007). Türkiye’de kalkan balığı avcılığının durumu ve sorunları. Su Ürünleri Mühendisleri Derneği Dergisi, 31, 17-20.
• Scheff, J.D., Almon, R.R., DuBois, D.C., Jusko, W.J., & Androulakis, I. P. (2011). Assessment of pharmacologic area under the curve when baselines are variable. Pharmaceutical research, 28(5), 1081-1089. http://doi.org/10.1007/s11095-010-0363-8.
• Shiry, N., Shomali, T., Soltanian, S., & Akhlaghi, M. (2019). Comparative single-dose pharmacokinetics of orally administered florfenicol in rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) at health and experimental infection with Streptococcus iniae or Lactococcus garvieae. Journal of veterinary pharmacology and therapeutics, 42(2), 214-221. http://doi.org/10.1111/jvp.12736.
• Smith, P. (2007). A survey of methods and protocols currently being used to determine antimicrobial susceptibility of bacteria associated with fish disease. Bulletin-European Association of Fish Pathologists, 27(1), 18-2.
• Smith, P., & Christofilogiannis, P. (2007). Application of normalised resistance interpretation to the detection of multiple low-level resistance in strains of Vibrio anguillarum obtained from Greek fish farms. Aquaculture, 272(1-4), 223-230.
• Taçbaş, E. (2018). Sağlıklı ve Lactococcus garvieae ile enfekte Gökkuşağı Alabalıkları (Oncorhynchus mykiss, Walbaum 1792)’nda Florfenikol’ün farmakokinetiği. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Farmakoloji ve Toksikoloji Anabilim Dalı Doktora Tezi, Ankara.
• Turner, P.V., Brabb, T., Pekow, C., & Vasbinder, M.A. (2011). Administration of substances to laboratory animals: Routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science, 50(5), 600-613.
• Türe, M., Haliloğlu, H.İ., Altuntaş, C., Boran, H., & Kutlu, İ. (2014). Comparison of experimental susceptibility of Rainbow Trout (Oncorhynchus mykiss), Turbot (Psetta maxima), Black Sea Trout (Salmo trutta labrax) and Sea Bass (Dicentrarchus labrax) to Lactococcus garvieae. Turkish Journal of Fisheries and Aquatic Sciences, 14(2), 507-513. https://doi.org/10.4194/1303-2712-v14_2_22.
• Rairat, T., Hsieh, C.Y., Thongpiam, W., Sung, C.H., & Chou, C.C. (2019). Temperature-dependent pharmacokinetics of florfenicol in Nile tilapia (Oreochromis niloticus) following single oral and intravenous administration. Aquaculture, 503, 483-488.
• Rigos, G., & Smith, P. (2015). A critical approach on pharmacokinetics, pharmacodynamics, dose optimisation and withdrawal times of oxytetracycline in aquaculture. Reviews in Aquaculture, 7(2), 77-106. https://doi.org/10.1111/raq.12055.
• van de Riet J. M., Potter R. A., Christie-Fougere M., & Burns B. G. (2003). Thiamphenicol, florfenicol, and florfenicol amine in farmed aquatic species by liquid chromatography/mass spectrometry, Journal of AOAC International, 23, 510-514.
• Vinarov, Z., Abdallah, M., Agundez, J., Allegaert, K., Basit, A. W., Braeckmans, M., Ceulemans, J., Corsetti, M., Griffin, B., Grimm, M., Keszthelyi, D., Koziolek, M., Madla, C. M., Matthys, C., McCoubrey, L. E., Mitra, A., Reppas, C., Stappaerts, J., Steenackers, N., Trevaskis, N. L., Vanuytsel, T., Vertzoni, M., Weitschies, W., Wilson, C., & Augustijns, P., (2021). Impact of gastrointestinal tract variability on oral drug absorption and pharmacokinetics: An UNGAP review. European Journal of Pharmaceutical Sciences, 162, 105812. http://doi.org/10.1016/j.ejps.2021.105812.
• Wang, W., Dai, X., Li, Z., & Meng, Q. (2009). Tissue distribution and elimination of florfenicol in Topmouth Culter (Culter alburnus) after Oral Administration. Czech Journal of Food Sciences, 27, 216-221.
• Yanong, P. R., Curtis, W. E., Simmons, R., Bhattaram, V. A., Gopalakrishnan, M., Ketabi, N., Nagaraja, N. V., & Derendorf, H. (2005). Pharmacokinetic studies of florfenicol in Koi Carp and threespot gourami Trichogaster trichopterus after oral and intramuscular treatment. Journal of Aquatic Health, 17(2), 129-137. https://doi.org/10.1577/H03-065.1.