Determination of the Pharmacokinetics of Florfenikol in Turbot (Psetta maxima) Fish Grown in Black Sea Water

Yıl 2022, Cilt: 11 Sayı: Supplement 1 - Veteriner Farmakoloji Kongresi Özel Sayısı, 15 - 21, 01.12.2022

Erkan Taçbaş , Emine Baydan , Mustafa Türe , İlyas Kutlu , Cemil Altuntaş , Gözde Yücel Tenekeci , Farah Gönül Aydın Emre Arslanbaş

https://doi.org/10.53424/balikesirsbd.1160570

Öz

Objective: In this study, it was aimed to determine the pharmacokinetics of florfenicol (FF) in a single dose (10 mg kg-1) in Black Sea Turbot (Psetta maxima). Materials and Methods: The study was carried out in closed system pools within the Trabzon Fisheries Central Research Institute in Black Sea water (temperature was 11±1ºC, the salinity rate was 0.18 %, oxygen did not fall below 10.02 mg ml-1). Experiments were performed on 65 turbot fish (42±2 g) including validation (5 specimens) and drug trials (60 specimens). Turbot fish were divided into two groups as FF administered intramuscularly and gavage. Determination of plasma FF levels was performed on a high pressure liquid chromatography device (HPLC). Pharmacokinetic analyzes were performed using the win-nonlin pharmacokinetic program according to the non-compartmental model. Results: The results of the validation studies were determined as 96.19, 85.81±0.026, 0.0039 and 0.012 based on Relative Standard Deviation (RSD) %, mean recovery %, LOD and LOQ in ppm, respectively. Tmax, Cmax, Tlast, Clast, AUClast, Vz_F_obs and Cl_F_obs values of intramuscular and gavage FF administration groups are respectively; 1 and 6 hour; 6.60 and 5.17 mg L-1; 48 and 48 hour; 2.02 and 1.48 mg L-1; 145.39 and 101.76 hour mg L-1; 2.54 and 3.64 L kg-1; 0.033 and 0.045 L kg h-1. Conclusion: The results of the study showed that the pharmacokinetics of FF may vary depending on the fish species and growing conditions, such as water temperature, salinity.

Anahtar Kelimeler

Pharmacokinetics, Florfenicol, Turbot, Black Sea, Intramuscular, Intragastric (gavage).

Proje Numarası

TAGEM / HSGYAD/15/A11/P03/65

Karadeniz Suyunda Yetişen Kalkan (Psetta maxima) Balıklarında Florfenikol’ün Farmakokinetiğinin Belirlenmesi

Öz

Amaç: Bu araştırmada Karadeniz Kalkan Balığında (Psetta maxima) tek doz (10 mg kg-1) florfenikolün (FF) farmakokinetiğinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çalışma Trabzon Su Ürünleri Merkez Araştırma Enstitüsü Müdürlüğü bünyesinde bulunan kapalı sistem havuzlarda Karadeniz suyunda (sıcaklık 11±1ºC, tuzluluk oranı %0.18, oksijen miktarı 10.02 mg ml-1 altına düşürülmedi) gerçekleştirildi. Deneyler validasyon (5 adet) ve ilaç denemeleri (60 adet) dahil 65 adet kalkan balığında (42±2 gr) gerçekleştirildi. Kalkan balıkları kas içi ve gavaj yoluyla FF uygulanan gruplar olmak üzere iki gruba ayrıldı. Plazma FF düzeylerinin belirlenmesi yüksek basınçlı sıvı kromatografisi cihazında (HPLC) gerçekleştirildi. Farmakokinetik analizler kompartımanlı olmayan modele göre win-nonlin farmakokinetik program kullanılarak yapıldı. Bulgular: Validasyon çalışma sonuçları Göreceli standart sapma (RSD) (%), ortalama geri kazanım (%), LOD ve LOQ (ppm) değerleri üzerinden sırasıyla, %96.19, %85.81±0.026, 0.0039 ve 0.012 ppm olarak belirlendi. Kas içi ve gavaj yoluyla FF uygulama gruplarının Tmax, Cmax, Tlast, Clast, AUClast, Vz_F_obs, Cl_F_obs değerleri sırasıyla 1 ve 6 saat; 6.60 ve 5.17 mg L-1; 48 ve 48 saat; 2.02 ve 1.48 mg L-1; 145.39 ve 101.76 saat mg L-1; 2.54 ve 3.64 L kg-1; 0.033 ve 0.045 L kg saat-1 olarak belirlendi. Sonuç: Araştırma sonuçları FF farmakokinetiğinin balık türü ve yetiştirme koşulları ile ilgili su sıcaklığı, tuzluluk oranı gibi faktörlere bağlı olarak değişebileceğini göstermiştir.

Anahtar Kelimeler

Farmakokinetik, Florfenikol, Kalkan, Karadeniz, Kas içi, Mide içi (gavaj).

Destekleyen Kurum

Tarım ve Orman Bakanlığı, Tarımsal Araştırma ve Politikalar Genel Müdürlüğü (TAGEM / HSGYAD/15/A11/P03/65).

Proje Numarası

TAGEM / HSGYAD/15/A11/P03/65

Teşekkür

Farmakokinetik analizlere katkılarından dolayı Cengiz GÖKBULUT’a, çalışmamız için ortam ve destek sağlaya Trabzon Merkez Su Ürünleri Araştırma Enstitüsü yönetimine ve personeline teşekkür ederiz.

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