Birecik (Türkiye)’te yaşayan Kelaynak popülasyonunun genetik yapısının mtDNA COI dizileri kullanılarak belirlenmesi

Abstract

İnsan nüfusunun hızla artması şehirleşmenin genişlemesine ve gıdaya olan ihtiyacın gün geçtikçe artmasına neden olmaktadır. Bu ihtiyaçlar birçok tarımsal faaliyetin oluşmasına yol açtığı için doğal ve yerli türlerin habitatlarını değiştirmektedir. Bu habitat değişimi özellikle hassas türlerin beslenme ve üreme alanlarının azalmasına neden olduğu için bu türlere ait popülasyonların azalmasına hatta yok olmasına neden olabilmektedir. Son yıllarda Birecik ilçesinde yaşayan ve nesli tehlike altında olan Kelaynak (Geronticus eremita) kuş türü hassas türlerden biri olup, koruma altına alınmıştır. Bu çalışmanın amacı, Birecik’te yaşayan ve koruma altına alınan bireylerin oluşturduğu popülasyona ait genetik yapının mtDNA COI bölgesine dayalı genetik analizinin yapılmasıdır. Bu bireylere ait tüylerden DNA izolasyonu yapılmış ve elde edilen total DNA’lar kullanılarak hedef mtDNA COI gen bölgesi çoğaltılmıştır. Daha sonra dizi analizleri yapılarak genetik yapı belirlenmiş ve gen bankasındaki sekanslar ile karşılaştırılarak analizler yapılmıştır. Çalışılan tüm bireylerin mtDNA COI gen bölgesine ait herhangi bir varyasyon tespit edilmemiştir. Gen bankasındaki dizilerle karşılaştırma yapılmış, filogenetik ağaç çizilmiş ve sonuçlar karşılaştırılarak haplotipler değerlendirilmiştir.

Keywords

• kelaynak
• genetik varyasyon
• Geronticus eremita
• Haplotip
• mtDNA COI

Determination of genetic structure of Bald ibis population living in Birecik (Turkey) using mtDNA COI sequences

Abstract

The rapid increase in the human population causes the expansion of urbanization and the need for food to increase day by day. As these needs lead to the formation of many agricultural activities, they change the habitats of natural and native species. Since this habitat change causes a decrease in the feeding and breeding areas of especially sensitive species, it may lead to the decrease or even extinction of the populations of these species. The endangered bald ibis (Geronticus eremita), bird species living in Birecik district, is one of the sensitive species and has been taken under protection in recent years. The endangered bald ibis (Geronticus eremita) bird species living in Birecik district in recent years is one of the sensitive species and has been taken under protection. The aim of this study is to perform genetic analysis of the genetic structure of the protected individuals, which live in Birecik, based on the mtDNA COI region. DNA was isolated from the feathers of these individuals, and the target mtDNA COI gene region was amplified by using the total DNAs obtained. Then, the genetic structure was determined by sequence analysis, and the analyzes were made by comparing them with the sequences in the gene bank. No variation was detected in the mtDNA COI gene region of all studied individuals. Comparisons were made with the sequences in the gene bank, a phylogenetic tree was drawn, and haplotypes were evaluated by comparing the results

Keywords

• Bald ibis,
• genetic variation
• haplotype
• Geronticus eremita
• mtDNA COI

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