Üç farklı manyetik alan uygulamasının M. offininalis tohum çimlenmesi ve fide büyümesi üzerine etkileri

Abstract

Oğul otu olarak da bilinen Melissa officinalis L. bitkisi, beyin ve merkezi sinir sistemi ile ilgili sorunlar, baş ağrıları, sinirlilik, sindirim bozuklukları, solunum ve dolaşım rahatsızlıkları, çeşitli kanser türleri ve romatizma tedavisinde kullanılmaktadır. Bu araştırma, M. officinalis tohum çimlenmesi (hızı ve başlangıcı) ve fide büyümesi (sürgün-kök uzunluğu, yaş-kuru ağırlık ve su içeriği) üzerine çeşitli maruz kalma süreleri (5 dk, 15 dk, 30 dk, 1 s ve 3 s) ile 3 farklı manyetik alan şiddetinin (50 mT, 100 mT ve 150 mT) etkilerinin karşılaştırılmasını amaçlamaktadır. Petri kaplarında yüzey sterilizasyonu yapılan tohumlar, çeşitli manyetik alan maruziyeti ve kontrol (maruz bırakmadan) olmak üzere farklı uygulamalara tabi tutulmuştur. Çimlenme, radiküler çıkıntı gözlemlendiğinde değerlendirilmiştir. Çimlenen tohumların sayısı 20 gün boyunca kaydedilmiştir. Üç manyetik alan uygulamasının hepsinde, 1 saat boyunca manyetik alana maruz kalma, kontrol (%28) ile karşılaştırıldığında en yüksek sayıda tohum çimlenmesine (50 mT, 100 mT ve 150 mT için sırasıyla %36, %52 ve %50) neden olmuştur. Tohum çimlenmesinin başlangıcı, manyetik alan uygulamasıyla (7. gün) kontrole (11. gün) kıyasla daha erken olmuştur. Farklı manyetik alan uygulamalarının etkisini anlamak için fidelerin büyüme elementleri ve su içerikleri karşılaştırılmıştır. 1 saat süreyle 100 mT ve 150 mT manyetik alan uygulaması fidelerin taze ağırlığına ek olarak gövde ve kök uzunluğunu artırmıştır. En fazla kuru ağırlık,artışı 150 mT-5 dk, 15 dk ve 30 dk ile sağlanmıştır. Kontrole (%44) kıyasla en yüksek su içeriği (%) 1 saat süreyle 150 mT (%68) ve 100 mT (%66) maruziyetinde gözlemlenmiştir. 1 saat boyunca 100 mT ve 150 mT manyetik alan yoğunluğu, tohum çimlenmesi ve fide büyümesinde en etkili olmuştur.

Keywords

• Çimlenme
• Manyetik alan
• Melissa officinalis

Project Number

BAP 2016.03.01.1027

Impacts of three different magnetic field applications on seed germination and seedling development of Melissa officinalis L.

Abstract

The herb Melissa officinalis L., most commonly recognized as lemon balm, has been used as a treatment for problems with the brain and central nervous system, headaches, nervousness, digestive disorders, respiratory and circulatory conditions, various types of cancer and rheumatism. This investigation was designated to compare the impacts of 3 discrete magnetic field strengths (50 mT, 100 mT and 150 mT) with various time periods of exposure (5 min, 15 min, 30 min, 1 hr and 3 hr) on M. officinalis seed germination (rate and onset) and seedling growth (length of shoot-root, weight of fresh-dry and water content). The seeds, which were surface sterilized in petri plates, were subjected to different applications, including exposure to various magnetic fields and control (without exposure). Germination was assessed by observing the radicular protrusion. The number of germinated seeds was recorded over 20 days. In all 3 magnetic field applications, exposure to magnetic field for 1 hr caused the highest number of seed germination (36%, 52% and 50% for 50 mT, 100 mT and 150 mT, respectively) comparing to control (28%). The onset of seed germination was earlier with magnetic field application (day 7) compared to control (day 11). Growth elements and water contents of the seedling were assessed in order to understand the impact of different magnetic field applications. Implementation of 100 mT and 150 mT magnetic field for 1 hr increased shoot and root length in addition to fresh weight of the seedlings. The maximum dry weight increase was provided by 150 mT-5 min, 15 min and 30 min. The highest water content (%) was observed with exposure to 150 mT (68%) and 100 mT (66%) for 1 hr compared to control (44%). Magnetic field intensity of 100 mT and 150 mT for 1 hr was the most efficient in the seed germination and seedling growth.

Keywords

• Germination
• Magnetic field
• Melissa officinalis

Supporting Institution

Bolu Abant İzzet Baysal Üniversity

Project Number

BAP 2016.03.01.1027

Thanks

We are grateful to Dr. Asaf Tolga Ülgen for his technical supports

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