Determination of phylogenetic relationships of two species of Phalangium (Opiliones: Phalangiidae) by using 28S rRNA region

Abstract

Abstract The species Phalangium nalanae Kurt, Erdek & Kurt 2023 and Phalangium taylani Kurt, Erdek & Kurt 2023 were previously described from Hakkari, Turkey based on morphological data and no molecular data are presented. In this study, the 28S rRNA gene region of these species belonging to the genus Phalangium was sequenced, and phylogenetic relationships of these species were revealed by performing maximum likelihood (ML) analysis and Bayesian inference (BI) analysis.

Keywords

• 28S rRNA
• Harvestmen
• Phylogenetic analyses

Thanks

Acknowledgements.We are very grateful to Dr. M. Kurnaz (Gümüşhane University, Türkiye) for his advice and valuable comments

28S rRNA gen bölgesi kullanılarak iki Phalangium (Opiliones: Phalangiidae) türünün filogenetik ilişkilerinin belirlenmesi

Abstract

Özet Phalangium nalanae Kurt, Erdek & Kurt 2023 ve Phalangium taylani Kurt, Erdek & Kurt 2023 türleri morfolojik verilere dayanarak daha önceden, Türkiye (Hakkâri)’den tanımlanmış ve türlere ait moleküler veriler sunulmamıştır. Bu çalışmada; Phalangium cinsine ait bu türlerin 28S rRNA gen bölgesi dizilenmiş, maksimum olabilirlik (ML) analizi ve Bayesian çıkarım (BI) analizi yapılarak bu türlerin filogenetik ilişkileri ortaya konulmuştur.

Keywords

• 28S rRNA
• filogenetik analiz
• otbiçen

References

1. [1] Mitov, P. G. (2012). Four new harvestmen records from Turkey (Arachnida: Opiliones). Serket, 13 (1/2): 73-82.
2. [2] Kurt, K., Erman, Ö.K., (2015) Harvestmen fauna of Gümüshane and Bayburt in Turkey (Arachnida, Opiliones). Spinaxiana, 38(1): 29-38.
3. [3] Kury, A.B., Mendes, A.C., Cardoso, L., Kury, M.S., Granado, A.de A. Giribet, G., Cruz-López J.A., Longhorn, S.J., Medrano, M., Oliveira, A.B.R. de, Kury, I.S. & Souza-Kury, M.A. (2023) World Catalogue of Opiliones. WCO-Lite version 2.6. Online at: https://wcolite.com/.
4. [4] Kurt, K. (2014a) Updated checklist of Harvestmen (Arachnida: Opiliones) in Turkey. Archives of Biological Sciences, Belgrade, 66 (4), 1617–1631. https://doi.org/10.2298/ABS1404617K
5. [5] Kurt, K., Koç, H. & Yağmur, E.A. (2015) Two new records for Turkish harvestmen fauna (Arachnida: Opiliones). Entomological News, 125(2), 127–135. https://doi.org/10.3157/021.125.0206
6. [6] Kurt K, Erdek M, Kurt P. (2023) Two new species of Phalangium (Opiliones: Phalangiidae) from Turkey. Zootaxa, 5360(3):429-436. https://doi.org/10.11646/zootaxa.5360.3.6
7. [7] Snegovaya, N. (2022). A new species of Phalangium (Opiliones, Phalangiidae) from Turkey. Zootaxa, 5169 (2), 197–200. https://doi.org/10.11646/zootaxa.5169.2.9
8. [8] Rix, M. G., Harvey, M. S., & Roberts, J. D. (2008). Molecular phylogenetics of the spider family Micropholcommatidae (Arachnida: Araneae) using nuclear rRNA genes (18S and 28S). Molecular phylogenetics and evolution, 46(3), 1031-1048.

9. [9] Giribet, G., Rambla, M., Carranza, S., Baguna, J., Riutort, M., & Ribera, C. (1999). Phylogeny of the arachnid order Opiliones (Arthropoda) inferred from a combined approach of complete 18S and partial 28S ribosomal DNA sequences and morphology. Molecular Phylogenetics and Evolution, 11(2), 296-307.
10. [10] Giribet, G., Vogt, L., Gonzalez, A. P., Sharma, P., & Kury, A. B. (2010). A multilocus approach to harvestman (Arachnida: Opiliones) phylogeny with emphasis on biogeography and the systematics of Laniatores. Cladistics, 26(4), 408-437. https://doi.org/10.1111/j.1096-0031.2009.00296.x
11. [11] Schönhofer, A. L., & Martens, J. (2010). Hidden Mediterranean diversity: Assessing species taxa by molecular phylogeny within the opilionid family Trogulidae (Arachnida, Opiliones). Molecular Phylogenetics and Evolution, 54(1), 59-75.
12. [12] Hedin, M., Tsurusaki, N., Macías-Ordóñez, R., & Shultz, J. W. (2012). Molecular systematics of sclerosomatid harvestmen (Opiliones, Phalangioidea, Sclerosomatidae): geography is better than taxonomy in predicting phylogeny. Molecular Phylogenetics and Evolution, 62(1), 224-236. https://doi.org/10.1016/j.ympev.2011.09.017
13. [13] Pinto‐da‐Rocha, R., Bragagnolo, C., Marques, F. P., & Antunes Junior, M. (2014). Phylogeny of harvestmen family Gonyleptidae inferred from a multilocus approach (Arachnida: Opiliones). Cladistics, 30(5), 519-539. https://doi.org/10.1111/cla.12065
14. [14] Giribet, G., Sheridan, K., Baker, C. M., Painting, C. J., Holwell, G. I., Sirvid, P. J., & Hormiga, G. (2021). A molecular phylogeny of the circum-Antarctic Opiliones family Neopilionidae. Invertebrate Systematics, 35(8), 827-849. https://doi.org/10.1071/IS21012
15. [15] Mallatt, J., & Sullivan, J. (1998) 28S and 18S rDNA sequences support the monophyly of lampreys and hagfishes. Molecular Biology and Evolution, 15, 1706–1718. https://doi.org/10.1093/oxfordjournals.molbev.a025897
16. [16] Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of molecular biology, 215(3), 403-410.
17. [17] Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
18. [18] Darriba, D., Taboada, G.L., Doallo, R., & Posada, D. (2012) JModelTest 2: Moremodels, new heuristics and parallel computing. Nature Methods, 9, 772. doi: 10.1038/nmeth.2109
19. [19] Akaike, H. (1974) A new look at the statistical model identification. IEEE Transactions on Automatic Control, 19, 716–723. https://doi.org/10.1109/TAC.1974.1100705
20. [20] Ronquist, F., Huelsenbeck, J., & Teslenko, M. (2011). Draft MrBayes version 3.2 Manual: Tutorials and Model Summaries, website. http://mrbayes.sour ceforge.net/manual.php.
21. [21] Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms., Molecular Biology and Evolution, 35:1547–1549. https://doi.org/10.1093/molbev/msy096
22. [22] Leaché, A.D., & Reeder, T.W. (2002) Molecular systematics of the eastern fence lizard (Sceloporus undulatus): a comparison of parsimony, likelihood, and Bayesian approaches. Systematic Biology, 51, 44–68. https://doi.org/10.1080/106351502753475871
23. [23] Pepato, A. R., & Klimov, P. B. (2015). Origin and higher-level diversification of acariform mites–evidence from nuclear ribosomal genes, extensive taxon sampling, and secondary structure alignment. BMC evolutionary biology, 15(1), 1-20. https://doi.org/10.1186/s12862-015-0458-2
24. [24] Hasegawa, M., Lida, Y., Yano, T., Takaiwa, F., Iwabuchi, M. (1985) Phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal RNA sequences. Journal of Molecular Evolution, 22:32–38.