Farklı Doz ve Boyuttaki BSA Nanopartiküllerinin Ankara Tekesi Spermasının Kriyoprezervasyonu Üzerindeki Etkileri: Motilite, Membranın Fonksiyonel Bütünlüğü ve Canlılık

Abstract

Amaç: BSA, 585 amino asit içeren ve yüksek oranda çözünebilir bir madde olup, uzun zamandır farklı türlerde spermanın dondurulmasında başarıyla kullanılmaktadır. Ancak, BSA'nın nanoformları (BSA NPs), yüksek biyoyararlanım kapasiteleri ve artan yüzey alanları sayesinde son yıllarda farklı hücrelerde ilaç taşıma uygulamalarında kullanılmaktadır. Bu çalışmada, BSA NPs'ın Ankara tekesi spermasının dondurulabilirliği üzerindeki etkileri incelenmiştir. Metod: Beş farklı Ankara tekesi sperması, 6 tekrar halinde toplanmış ve kontrol, BSA 20-5, BSA 20-10, BSA 40-5 ve BSA 40-10 olacak şekilde 5 gruba ayrılmıştır. Sıvı azot buharında dondurulan spermalar, 37 ºC'de çözdürülmüş ve motilite, membranın fonksiyonel bütünlüğü (HOST) ve canlı/ölü oranları değerlendirilmiştir. Bulgular: Elde edilen veriler, motilite ve canlı/ölü oranı açısından gruplar arasında istatistiksel olarak anlamlı bir fark olmadığını göstermektedir. Ancak, HOST testi sonucunda, kontrol grubu ve BSA 20-5 grubu diğer gruplara oranla daha yüksek membran dayanım kabiliyetine sahip bulunurken, BSA 20-10, BSA 40-5 ve BSA 40-10 gruplarındaki spermaların membran dayanım kabiliyeti düşmüştür. Bu durumun, BSA NPs'ın membrandaki kolesterolü delokalize etmesinden kaynaklandığı düşünülmektedir. Sonuç: Sonuç olarak, düşük doz ve boyuttaki BSA NPs'ın spermaya zarar vermediği ve ilaç taşıma sistemlerinde kullanım potansiyeli taşıdığı sonucuna varılmıştır.

Keywords

• BSA
• nanoparçacık
• sperm
• dondurma
• membran
• ilaç taşınımı

Supporting Institution

Erciyes University Scientific Research Projects Coordination Unit

Project Number

TSA-2022-12015

Ethical Statement

Acknowledgement: We would like to thank Erciyes University Scientific Research Projects Coordination Unit for supporting the project with the number TSA-2022-12015. We would also like to thank the Directorate of Selcuk University Veterinary Faculty, Humeyra Özgen Research and Application Farm, where the Angora bucks used in the project were maintained and fed. Conflicts of interest: The authors of this study declare that they have no conflicts of interest with any institutions or organizations. Funding: This project was funded by the Erciyes University Scientific Research Projects Coordination Unit under project number TSA-2022-12015. Ethical statement: The ethical approval for this study was granted by the Ethics Committee of Selçuk University, Faculty of Veterinary Medicine, Animal Research and Production Center, with decision number 2022/40 dated 06.05.2022. Author contributions: Ali Erdem ÖZTÜRK, Yunus Emre ATAY and Mustafa BODU designed the research study. Ali Erdem ÖZTÜRK, Mustafa BODU and Zeliha KILINÇ performed the research. Serpil SARIÖZKAN collected and analyzed the data. İsmail ÖÇSOY has been involved in drafting the manuscript. All authors give final approval of the version to be published. This study was presented as an oral presentation at the conference titled ‘Elegans International Scientific Conference Series, First International Conference on Life Science’ and its abstract was published in the conference book.

Thanks

We would like to thank Erciyes University Scientific Research Projects Coordination Unit for supporting the project with the number TSA-2022-12015. We would also like to thank the Directorate of Selcuk University Veterinary Faculty Humeyra Özgen Research and Application Farm where the ankara goats used in the project were maintained and fed.

Effects of different doses and sizes of BSA nanoparticles on cryopreservation of angora buck semen: motility, functional membrane ıntegrity and, viability

Abstract

Purpose: BSA is a highly soluble substance consisting of 585 amino acids, which has been successfully used for the cryopreservation of sperm from various species for a long time. However, the nanoforms of BSA (BSA NPs) have been utilized in drug delivery applications in different cell types in recent years due to their high bioavailability and increased surface areas. In this study, we investigate the effects of BSA NPs on the cryopreservability of Angora buck sperm. Method: Sperm samples from five different Ankara rams were collected in six replicates and divided into five groups: control, BSA 20-5, BSA 20-10, BSA 40-5, and BSA 40-10. The sperm, frozen in liquid nitrogen vapor, were thawed at 37 ºC, and motility, functional membrane integrity (HOST), and live/dead ratios were assessed. Findings: The data obtained showed no statistically significant difference among the groups in terms of motility and live/dead ratios. However, in the HOST test, the control group and the BSA 20-5 group had higher resistance capabilities compared to the other groups, while BSA 20-10, BSA 40-5, and BSA 40-10 groups reduced membrane resistance capability. This situation is considered to result from the removal of cholesterol from the membrane by BSA nanoparticles. Conclusion: Consequently, the study concludes that BSA NPs used at low doses and sizes do not harm sperm and hold promise for use in drug delivery systems.

Keywords

• BSA
• nanoparticle
• sperm
• freezing
• membrane
• drug delivery.

Project Number

TSA-2022-12015

Ethical Statement

Acknowledgement: We would like to thank Erciyes University Scientific Research Projects Coordination Unit for supporting the project with the number TSA-2022-12015. We would also like to thank the Directorate of Selcuk University Veterinary Faculty, Humeyra Özgen Research and Application Farm, where the Angora bucks used in the project were maintained and fed. Conflicts of interest: The authors of this study declare that they have no conflicts of interest with any institutions or organizations. Funding: This project was funded by the Erciyes University Scientific Research Projects Coordination Unit under project number TSA-2022-12015. Ethical statement: The ethical approval for this study was granted by the Ethics Committee of Selçuk University, Faculty of Veterinary Medicine, Animal Research and Production Center, with decision number 2022/40 dated 06.05.2022. Author contributions: Ali Erdem ÖZTÜRK, Yunus Emre ATAY and Mustafa BODU designed the research study. Ali Erdem ÖZTÜRK, Mustafa BODU and Zeliha KILINÇ performed the research. Serpil SARIÖZKAN collected and analyzed the data. İsmail ÖÇSOY has been involved in drafting the manuscript. All authors give final approval of the version to be published. This study was presented as an oral presentation at the conference titled ‘Elegans International Scientific Conference Series, First International Conference on Life Science’ and its abstract was published in the conference book.

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