Türkiye’deki Biarum Cinsine Ait Bazı Türlerin Plastid rps16 DNA dizilerine dayalı Filogenetik Analizi

Abstract

DNA’sında bulunan rps16 bölgesine dayalı dizi analizlerini tespit etmek amacıyla yapılmıştır. Metod: Türkiye’nin farklı lokalitelerinde doğal yayılış gösteren 8 Biarum türü toplanarak DNA izolasyonu kit kullanılarak gerçekleştirilmiş ve Polimeraz Zincir Reaksiyonu (PCR) ile rps16 bölgesi çoğaltılmıştır. DNA dizileri analiz edilerek türler arasındaki genetik mesafeler hesaplanmış ve Neighbor-Joining yöntemi kullanılarak filogenetik ağaç oluşturulmuştur. Bulgular: Analizler sonucunda, Biarum bovei ve B. crispulum türlerinin en yakın genetik akraba olarak belirlenirken, Biarum davisii subsp. marmarisense ve B. bovei en uzak akraba türler olarak tespit edilmiştir. Elde edilen genetik analiz sonuçlarının, morfolojik verilere dayalı sınıflandırmayı doğrulamakta ve Biarum türlerinin filogenetik yapısının genetik analizlerle desteklenmesi gerektiğini ortaya koymaktadır. Sonuç: İlk defa bu çalışma ile Biarum cinsine ait genetik veriler elde edilmiş olup gelecekte, coğrafi ve ekolojik faktörlerin de dahil olduğu daha kapsamlı markörlerin de kullanılarak filogenetik çalışmaların yapılması önerilmektedir.

Keywords

• Kloroplast DNA
• rps16
• filogenetik
• Biarum

Phylogenetic analysis of some species of the Biarum genus in Turkey based on plastid rps16 DNA sequences

Abstract

Purpose: In this study, phylogenetic relationships of some species of Biarum genus naturally distributed in Turkey were determined by sequence analyses based on rps16 region in chloroplast DNA. Method: 8 Biarum species naturally distributed in different localities of Turkey were collected and DNA isolation was performed using a kit, and rps16 region was amplified by Polymerase Chain Reaction (PCR). Genetic distances between species were calculated by analyzing DNA sequences and a phylogenetic tree was constructed using the Neighbor-Joining method. Findings: As a result of the analyses, Biarum bovei and B. crispulum species were identified as the closest genetic relatives, while Biarum davisii subsp. marmarisense and B. bovei were determined as the most distant relatives. The obtained genetic analysis results confirm the classification based on morphological data and reveal that the phylogenetic structure of Biarum species should be supported by genetic analyses. Conclusion: This study provides one of the first phylogenetic analyses based on the rps16 chloroplast region in Turkish Biarum species and it is recommended that future phylogenetic studies be conducted using more comprehensive markers including geographical and ecological factors.

Keywords

• Chloroplast DNA
• rps16
• phylogenetics
• Biarum

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