Antiproliferative and cytotoxic activity of Papaver somniferum seed oil on human breast cancer cell lines

Abstract

Background: This study aimed to determine the antiproliferative and cytotoxic activities of Papaver somniferum seed oil on human breast cancer (MDA-MB-231) cell lines under in vitro conditions. Methods: Poppy seed oil extracted by cold press method were subjected to compositional analysis using GC-MS. In the study, control group, DMSO (0.1%) applied group and 5 different concentrations of Poppy seed oil applied group three main treatment groups were created. Cell viability was investigated by the trypan blue method. Some pharmacokinetic properties and toxicity profiles of the identified molecules were predicted using a Swiss ADME online server and ProTox-II webserver. Results: The effect of Poppy seed oil on breast cancer cells increased relatively starting from the lowest concentration (87 µg/ml), and it was determined that the effect became more pronounced after a concentration of 350 µg/ml. Except Hexadecanoic acid and (E)-9- Octadecanoic acid, all other compounds were predicted to be non-hepatotoxic, non-carcinogenic, non-cytotoxic, non-immunotoxic and non-mutagenic. All compounds investigated are orally absorbable, suitable for dermal trans-distribution, have high gastrointestinal absorption, however, Hexadecanoic acid is permeable to the blood-brain barrier (BBB), while the other 4 compounds are not. The bioavailability values of all compounds investigated can be considered to be very well absorbed by the body. Conclusion: In this study, it was determined that P. somniferum seed oil has antiproliferative and cytotoxic activity on breast cancer cells in vitro. P. somniforum seed oil may be a natural anticancer agent to treat breast cancer, but more studies are needed on this subject.

Keywords

• breast cancer
• cytotoxicity
• Papaver somniferum
• poppy

Ethical Statement

Conflicts of interest: The authors have no conflicts of interest to declare. Financial declaration: The authors have no relevant financial or non-financial interests to disclose. Authors' contributions: All authors contributed to the study conception and design. All authors read and approved the final manuscript.

Papaver somniferum tohum yağının insan meme kanseri hücre hatları üzerinde antiproliferatif ve sitotoksik aktivitesi

Abstract

Amaç: Bu çalışmada, Papaver somniferum tohum yağının insan meme kanseri (MDA-MB-231) hücre hatları üzerindeki antiproliferatif ve sitotoksik aktivitelerinin in vitro koşullarda belirlenmesi amaçlanmıştır. Metod: Soğuk pres yöntemiyle ekstrakte edilen haşhaş tohumu yağı, GC-MS kullanılarak kompozisyon analizine tabi tutulmuştur. Çalışmada, kontrol grubu, DMSO (%0,1) uygulanan grup ve 5 farklı konsantrasyonda haşhaş tohumu yağı uygulanan grup olmak üzere üç ana tedavi grubu oluşturulmuştur. Hücre canlılığı tripan mavisi yöntemi ile araştırılmıştır. Tanımlanan moleküllerin bazı farmakokinetik özellikleri ve toksisite profilleri, bir İsviçre ADME çevrimiçi sunucusu ve ProTox-II web sunucusu kullanılarak tahmin edilmiştir. Bulgular: Haşhaş tohumu yağının meme kanseri hücreleri üzerindeki etkisi en düşük konsantrasyondan (87 µg/ml) başlayarak nispeten artmış ve etkinin 350 µg/ml konsantrasyonundan sonra daha belirgin hale geldiği belirlenmiştir. Heksadekanoik asit ve (E)-9- Oktadekanoik asit hariç, diğer tüm bileşiklerin hepatotoksik, kanserojen, sitotoksik, immünotoksik ve mutajenik olmadığı öngörülmüştür. İncelenen tüm bileşikler oral yoldan emilebilir, dermal trans-dağılım için uygundur ve yüksek gastrointestinal emilime sahiptir; ancak Heksadekanoik asit kan-beyin bariyerine (BBB) geçirgenken, diğer 4 bileşik geçirgen değildir. İncelenen tüm bileşiklerin biyoyararlanım değerlerinin vücut tarafından çok iyi emildiği düşünülebilir. Sonuç: Bu çalışmada, P. somniferum tohum yağının in vitro meme kanseri hücreleri üzerinde antiproliferatif ve sitotoksik aktiviteye sahip olduğu belirlenmiştir. P. somniforum tohum yağının meme kanserini tedavi etmek için doğal bir antikanser ajanı olabileceği, ancak bu konuda daha fazla çalışmaya ihtiyaç duyulduğu belirtilmiştir.

Keywords

• meme kanseri
• sitotoksisite
• Papaver somniferum
• haşhaş
• biyoaktivite

Ethical Statement

Conflicts of interest: The authors have no conflicts of interest to declare. Funding: No funding received. Ethical statement: This study does not require ethical approval. Financial declaration: The authors have no relevant financial or non-financial interests to disclose. Authors' contributions: All authors contributed to the study conception and design. All authors read and approved the final manuscript.

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