In vitro propagation of Alhagi maurorum (Fabaceae)

Abstract

In vitro propagation of Alhagi maurorum (Fabaceae) Mustafa CUCE 1 ORCID: 0000-0001-7406-171X 1 Department of Food Technology, Şebinkarahisar School of Applied Sciences, Giresun University, 28400 Giresun, Türkiye Abstract Purpose: In this study, a contribution was made to a reliable and reproducible in vitro propagation protocol of the rare plant species Alhagi maurorum, which belongs to the Fabaceae family and is of medicinal importance. Method: Mature seeds were used as the initial material. In germination studies, MS medium without any plant growth regulator and MS containing 1.0 mg L-1 GA3 were preferred as the basic medium. At the end of the germination culture period (4 weeks), MS medium supported with 1.0 mg L-1 GA3 was found to be superior with 60% germination success. In shoot multiplication studies, nodal segments obtained at the end of the 4th subculture were used as explants, and MS basal medium was tested by combining 6-BA, kinetin, 2iP, zeatin and TDZ (1.0 mg L-1 separately with IBA (0.1 mg L-1). Findings: The highest mean values per explant for shoot number (4.75), node number (7.50), fresh weight (0.985 g), and dry weight (0.1171 g) were obtained from MS medium containing zeatin + IBA. MS medium supplemented with kinetin + IBA reached the highest mean shoot length with 69.26 mm. In terms of root induction, the highest rooting percentage (90.3%) was obtained in MS media enriched with 1.0 mg L-1 IBA after 4 weeks. This concentration of IBA yielded the highest root number and root length with 5.86 and 38.80 respectively, in MS medium. Rooted plants were initially acclimatized in a climate chamber and then carefully transferred to greenhouse conditions. Conclusion: This propagation method can be considered for ex situ conservation and propagation of rare plant species of medicinal value. Keywords: Alhagi maurorum, rare endemic, zeatin, in vitro propagation

Keywords

• Alhagi maurorum
• rare endemic
• zeatin
• in vitro propagation

Alhagi maurorum'un (Fabaceae) in vitro çoğaltılması

Abstract

Alhagi maurorum'un (Fabaceae) in vitro çoğaltılması Özet Amaç: Fabaceae familyasında yer alan ve tıbbi açıdan önemli bir tür olan nadir Alhagi maurorum’un güvenilir ve tekrarlanabilir bir in vitro çoğaltma protokolüne bu çalışmada katkıda bulunulmuştur. Metod: Başlangıç materyali olarak olgun tohumlar kullanılmıştır. Çimlendirme çalışmalarında temel besi ortamı olarak herhangi bir bitki büyüme düzenleyicisi içermeyen ve 1,0 mg L-1 GA3 ile içeren MS tercih edilmiştir. Çimlendirme kültür süresi sonunda (4 hafta) 1,0 mg L-1 GA3 ile desteklenen MS ortamı %60 çimlenme başarısı ile daha üstün bulunmuştur. Sürgün çoğaltma çalışmalarında 4. alt kültür sonunda elde edilen nodal segmentler eksplant olarak kullanılmış, 6-BA, kinetin, 2iP, zeatin ve TDZ (1.0 mg L-1) IBA (0,1 mg L-1) ile ayrı ayrı kombine edilerek MS temel besi ortamında test edilmiştir. Bulgular: Eksplant başına ortalama en yüksek sürgün sayısı (4,75), nod sayısı (7,50), yaş ağırlık (0,985 g) ve kuru ağırlık (0,1171 g) değerleri zeatin + IBA içeren MS ortamından elde edilmiştir. Sadece kinetin + IBA ile desteklenmiş MS besi ortamı 69,26 mm ile en yüksek sürgün uzunluğuna ulaşmıştır. Kök indüksiyonu açısından, en yüksek köklenme yüzdesi (%90,3), 4 hafta sonra 1,0 mg L-1 IBA ile zenginleştirilmiş MS ortamında elde edilmiştir. Bu IBA konsantrasyonu, sırasıyla 5,86 ve 38,80 ile en yüksek kök sayısını ve kök uzunluğunu vermiştir. Köklenen bitkiler önce bir iklim odasında iklimlendirilmiş, ardından sera koşullarına aktarılmıştır. Sonuç: Bu çoğaltma yöntemi, tıbbi değeri olan nadir bitki türlerinin ex situ muhafazası ve çoğaltılması için de düşünülebilir. Anahtar kelimeler: Alhagi maurorum, nadir endemik, zeatin, in vitro çoğaltım

Keywords

• Alhagi maurorum
• nadir endemik
• zeatin
• in vitro çoğaltım

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