Vinca soneri l. metanol ekstraktının fitokimyasal profili ve biyolojik aktiviteleri

Abstract

Vinca soneri l. metanol ekstraktının fitokimyasal profili ve biyolojik aktiviteleri Özet Amaç: Bu çalışmada, Vinca soneri Koyuncu bitkisinin toprak üstü kısımlarından metanol ekstresi hazırlanmış ve LC-MS/MS analizleri ile kimyasal karakterizasyonu yapılmıştır. Metod: V. soneri örnekleri Erzincan’dan toplanmış; toprak üstü kısımları gölgede kurutulup toz haline getirilmiştir. Toz halindeki materyal oda sıcaklığında iki kez metanol ile ekstrakte edilmiş ve elde edilen ekstreler, vakum altında ve 40°C'de döner buharlaştırıcı (rotary evaporatör) kullanılarak uçurulmuştur. Bulgular: Çalışmada, başta fenolik asitler (kinik asit, protokateşuik asit, klorojenik asit, protokateşuik aldehit, rozmarinik asit ve kafeik asit), flavonoidler (rutin, kuersetin, izokuersetrin, hesperidin, akasetin ve luteolin) ve flavonoid glikozitleri (nikotiflorin) olmak üzere çeşitli ikincil metabolitlerin içeriği belirlenmiştir. Ayrıca ekstrelerin antioksidan kapasiteleri (DPPH ve ABTS radikal süpürme testleri) ile kolinesteraz, tirozinaz, elastaz ve kolajenaz enzimlerine karşı inhibitör özellikleri incelenmiştir. Sonuç: Elde edilen bulgular, V. soneri bitkisinin kimyasal ve biyolojik özelliklerine dair detaylı veri sunmakta ve nutrasötikler ile gıda takviyelerinin geliştirilmesi için bilimsel bir temel oluşturmaktadır.

Keywords

• Vinca soneri
• antioksidanlar
• enzim inhibitör özellikleri
• LC-MS/MS.

Phytochemical profile and biological activities of Vinca soneri Koyuncu methanol extract

Abstract

Purpose: In the present study, methanol extract was prepared from the aerial parts of Vinca soneri and has been chemically characterized by LC-MS/MS analyses. Method: V. soneri was collected from the Erzincan, the aerial parts were dried in the shade and powdered. The powdered material was extracted with methanol twice at room temperature, and they were evaporated by rotary evaporator at 40°C under vacuum. Findings: Notably, the content of some secondary metabolites, namely phenolic acids (quinic acid, Protocatechuic acid, Chlorogenic acid, Protocatechuic aldehyde, rosmarinic acid and caffeic acids), flavonoids (rutin, quercetin, isoquercitrin, hesperidin, acacetin, and luteolin), flavonoid glycosides (nicotiflorin), has been determined. Furthermore, the extracts were tested for their antioxidant capabilities (DPPH, and ABTS radical scavenging assays) and enzyme inhibitory properties against cholinesterase, tyrosinase, elastase, and collagenase. Conclusion: Our findings present data for chemico-biological characteristics of V. soneri and give a scientific foundation for the development of nutraceuticals and food supplements.

Keywords

• Vinca soneri
• antioxidants
• enzyme inhibitory properties
• LC-MS/MS.

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