Siyah üzüm ekstresinin meme kanseri hücrelerinde MMP-9 gen ekspresyonuna etkisi

Abstract

Meme kanseri sağ kalım oranındaki düşüş ve tedavisindeki başarısızlığın en önemli etkenleri arasında metastaz görülmektedir. Meme kanseri metastazının moleküler yolaklarının aydınlatılması büyük bir öneme sahiptir. Son yıllarda yapılan araştırmalar, bitkilerde bulunan polifenolik bileşiklerin anti-invaziv ve anti-metastatik yeteneklerine sahip olduğunu göstermektedir. Bol miktarda polifenol içeren siyah üzüm ekstresinin meme kanseri ve metastazı üzerindeki etkisi ve moleküler mekanizması henüz tam olarak bilinmemektedir. Çalışmamızda, insan meme kanseri hücre dizisine uygulanan siyah üzüm ektresinin hücre canlılığı ve adezyonuna etkisi ile metastazda rolü olabileceği düşünülen MMP-9 gen ekspresyonuna etkisinin belirlenmesi amaçlanmıştır. MCF-7 meme kanser hücre dizilerinde tripan blue yöntemi ile hücre canlılığı, XTT yöntemi ile hücre adezyonu ve qRT-PCR ile MMP-9 gen ekspresyonu belirlenmiştir. Çalışmamızda, siyah üzüm ekstresinin MMP-9 gen ekspresyonunu, hücre canlılığını ve adezyonunu (p>0.05) azalttığı bulunmuştur. Sonuç olarak polifenoller gibi doğal kanser önleyici ajanların kanserin oluşmasını ve yayılmasını önleyebileceği fikri desteklenmiştir. Bununla birlikte polifenollerin etkilediği moleküler mekanizmaların aydınlatılabilmesi için ileri çalışmalara ihtiyaç vardır.

Keywords

• Meme kanseri
• Üzüm ekstresi
• MMP-9
• Gen ekspresyonu

Effect of black grape extract on MMP-9 gene expression in breast cancer cells

Abstract

Metastasis is among the most important factors in the decrease in breast cancer survival and treatment failure. It is of great importance to elucidate the molecular pathways of breast cancer metastasis. Recent research shows that polyphenolic compounds in plants have anti-invasive and anti-metastatic activities. Molecular mechanism of black grape extract including abundant polyphenols on breast cancer and metastasis are not yet fully known. In our study, it was aimed to determine the effect of black grape extract applied to human breast cancer cell line on cell viability and adhesion and on MMP-9 gene expression, which is thought to have a role in metastasis. In the study, cell viability by trypan blue method, cell adhesion with XTT method and MMP-9 gene expression by qRT-PCR were determined in MCF-7 breast cancer cell lines. In our study, it was found that black grape extract decreased MMP-9 gene expression, cell viability and adhesion (p> 0.05). The study results support the hypothesis that natural anti-cancer agents such as polyphenols can prevent cancer from occurring and spreading. However, further studies are needed to elucidate the molecular mechanisms of polyphenols.

Keywords

• Breast cancer
• Grape extract
• MMP-9
• Gene expression

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