PLANT-DERİVED VACCİNES

Abstract

Plants are platforms where recombinant proteins and other biopharmaceuticals can be produced easily, cheaply and safely and can be purified back. Recently, many recombinant proteins such as growth hormone, antibody milk proteins, serum albumin and various industrial enzymes produced in bacterial or mammalian cell cultures have been produced in plant tissue or in plant cell culture. Plant tissues provide suitable post-translational modifications for production of recombinant viral and bacterial antigens and show the same biological activity as the recombinant vaccines produced in microorganisms. All of these have paved the way for their usage in vaccine production. Production of recombinant protein in plants requires stable or transient integration of target gene sequence depending on the location in the plant cell. While the biolistic method is used for the stable transformation of the target gene in the nucleus or chloroplast, plant pathogen Agrobacterium sp. mediated gene transfer method is used for transient gene transfer. Plants are extremely suitable expression vectors for industrial production of pharmaceutical proteins, with their proven production capacity and economic feasibility.

Keywords

• Plant derived vaccines
• genetic modification
• plant biotechnology

Bitkisel türevli aşılar

Abstract

Bitkiler, rekombinant proteinlerin ve diğer biyofarmasotiklerin kolay, ucuz ve güvenli üretiminin sağlanabildiği ve geri saflaştırılabildiği platformlardır. Günümüzde bakteri ya da memeli hücre kültürlerinde üretilen büyüme hormonu, antikor süt proteinleri, serum albumini ve çeşitli endüstriyel enzimler gibi birçok rekombinant proteinin bitkisel dokuda ya da bitki hücre kültüründe üretimi gerçekleştirilmiştir. Bitkisel dokuların rekombinant viral ve bakteriyel antijenlerin üretimleri için uygun post-translasyonel modifikasyonları sağlamaları ve mikroorganizmalarda üretilen rekombinant aşılar ile aynı biyolojik aktiviteyi göstermeleri, aşı üretiminde kullanılmalarının önünü açmıştır. Bitkisel rekombinant protein üretimi, hedef gen dizisinin bitki hücresindeki konumuna bağlı olarak stabil veya geçici entegrasyonunu gerektirir. Biyolistik yöntemi hedef genin çekirdek veya kloroplastta stabil transformasyonu için kullanılırken, bir bitki patojeni olan Agrobacterium sp. aracılı gen transferi yöntemi, geçici gen transferi için kullanılmaktadır. Bitkisel sistemler kanıtlanmış üretim kapasiteleri ve ekonomik fizibiliteleri ile farmasotik proteinlerin endüstriyel boyutta üretimleri için son derece uygun ekspresyon vektörleridir.

Keywords

• Bitkisel aşılar
• genetik modifikasyon
• bitki biyoteknoloji

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