Mentha longifolia subsp. longifolia L. HUDSON (Lamiaceae)’dan elde edilen uçucu yağın Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae) üzerine Beslenme engelleyici etkileri

Abstract

Türkiye’de Mentha longifolia subsp. longifolia L. HUDSON (Lamiaceae)‘dan elde edilen uçucu yağların Ordu ilinden toplanan S. vigintiquatuorpunctata (Coleoptera: Coccinellidae) üzerine antifeedant (beslenmeyi engelleyici) etkisi araştırılmıştır. Zararlı böceklere karşı bitkilerin korunması genellikle kimyasal insektisitler kullanılarak yapılmaktadır. Fakat kimyasal insektisit kullanımı ekosistem için ciddi riskler oluşturmaktadır. Son zamanlarda bilim adamları, kimyasal insektisitler yerine bitkilerden elde edilen uçucu yağların (fitokimyasalların) kullanımı üzerine araştırmalara odaklanmışlardır. Benzer şekilde, M. longifolia subsp. longifolia L.‘dan elde edilen uçucu yağ 5 farklı dozda (312,5, 625, 1250, 2500 ve 5000 ppm) hazırlanmıştır. Sonuç olarak M. longifolia subsp. longifolia L.‘dan elde edilen uçucu yağların S. vigintiquatuorpunctata türü üzerinde güçlü bir antifeedant etkiye sahip olduğu belirlenmiştir. Mentha longifolia bitkisinden elde edilen uçucu yağın artan derişimleri ile antifeedant etki arasında pozitif korelasyon korelasyon (r = 0,733, P < 0,005) belirlenmiştir. Antifeedant etki 5000 ppm derişiminde maximuma ulaşmıştır. 5000 ppm için AFI değeri 71,88± 6,4 tür. Buna ek olarak, GC-MS ile M. longifolia subsp. longifolia yapraklarından elde edilen uçucu yağın kimyasal bileşenleri belirlenmiştir. M. longifolia subsp. longifolia bitkisinden elde edilen uçucu yağda ana bileşen olarak %39,58 oranında 2-Cyclohexen-1-one ve ikinci olarak ta %16,97 oranında p-Menthone tespit edilmiştir.

Keywords

• antifeedant
• Mentha longifolia
• Subcoccinella vigintiquatuorpunctata
• uçucu yağ

Antifeedant Effects of Essential oil of Mentha longifolia subsp. longifolia L. HUDSON (Lamiaceae) on Subcoccinella vigintiquatuorpunctata L. (Coleoptera: Coccinellidae)

Abstract

Antifeedant activity of essential oils obtained from Mentha longifolia subsp. longifolia L. HUDSON (Lamiaceae) against S. vigintiquatuorpunctata (Coleoptera: Coccinellidae) adults that are collected from Ordu were tested in Turkey. In general, protection of plants against pests is performed using chemical insecticides, However the use of chemical insecticides on control of the insects creates serious risks on the ecosystem. Lately, scientists are focused on studies about using essential oils obtained from plants (phytochemicals) as alternatives to chemicals insecticides for pest control. Similarly essential oils obtained from M. longifolia subsp. longifolia L. was prepared in 5 different doses (312.5, 625, 1250, 2500 and 5000 ppm). As a result, essential oils obtained from M. longifolia subsp. longifolia L. were seen to have strong antifeedant effects. It were determined positive correlation (r = 0.733, P < 0.005) between increasing concentrations of the essential oils obtained from Mentha longifolia L. and AFI indices. The AFI value reached a maximum at 5000 ppm. The AFI value for 5000 ppm is 71.88 ± 6.4. Additionally, chemical composition of essential oil of M. longifolia subsp. longifolia leaves were determined by GC-MS analysis. The main component of essential oil of M. longifolia subsp. longifolia was 2-Cyclohexen-1-one (39.58) and second one was p-Menthone (16.97%).

Keywords

• antifeedant
• Mentha longifolia
• Subcoccinella vigintiquatuorpunctata
• essential oil

References

1. Wheeler, A.G., Henry, T.J. (1981). Seasonal history and habits of the European alfalfabeetle, Subcoccinella vigintiquatuorpunctata (L.) (Coleoptera: Coccinellidae). Coleopterists Bulletin, 35, 197–203.
2. Başaran, U., Acar, Z., Mut, H., Aşcı, Ö.Ö. (2006). Doğal Olarak Yetişen Bazı Baklagil Yembitkilerinin Bazı Morfolojik ve Tarımsal Özellikleri. Journal of the Faculty of Agriculture, 21 (3),314–317.
3. Lemus, I., Garcia, R., Delvillar, E., Knop, G. (1999). Hypoglycaemic activity of four plants used in chilean popular medicine. Phytotherapy Research, 13, 91–94.
4. Mossa, A. (2016). Green Pesticides: Essential Oils as Biopesticides in Insect-pest Management, Journal of Environmental Science and Technology, 9(5):354-378. http://doi:10.3923/jest.2016.354.378
5. Isman, M. B. (1995). Leads and prospects for the development of new botanical insecticides. Rev. Pesticide Toxicol, 3, 1-20.
6. Taiz, L. and Zaiger, E. (2002). Plant physiology, Third Edition. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts. p. 690.
7. Alpkent, Y. N., Alaoğlu, Ö. and Çetin, H. (2013). Fumigant toxicity of some plant essential oils to Ephestia kuehniella Zeller (Lepidoptera : Pyralidae). Bitki Koruma Bülteni, 53(2): 115-126.
8. Bekircan, Ç., Cüce, M. and Sökmen, A. (2014). Antifeedant Activity of the Essential Oils from Four Different Lamiaceae Species against Agelastica alni L. (Coleoptera Chrysomelidae). Advances in Zoology and Botany, 2(4): 57-62. http://doi:10.13189/azb.2014.020401

9. Bekircan, Ç., Cüce, M., Bekircan, T. and Tosun, O. (2015). Role of Different Thymus Essential Oils on Feeding Performance of Leptinotarsa decemlineata Say, 1824 (Chrysomelidae Coleoptera). Advances in Zoology and Botany, 3(1): 6-9. http://doi: 10.13189/azb.2015.030102
10. Motazedian, N., Ravan, S. and A. Bandani, R. (2012). Toxicity and Repellency Effects of Three Essential Oils against Tetranychus urticae Koch (Acari: Tetranychidae). Journal of Agriculture Science and Technology, 14: 275-284.
11. Odeyemi, O. O., Masika, P. and Afolayan, A. J. (2008). Insecticidal activities of essential oil from the leaves of Mentha longifolia L. subsp. capensis against Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae). Entomological Society of Southern Africa,16(2) : 220-225.
12. Singh, P. and Pandey, A. K. (2018) .Prospective of Essential Oils of the Genus Mentha as Biopesticides. Frontiers in Plant Science, 9:1-14. https://doi.org/10.3389/fpls.2018.01295
13. D’Incao, M. P., Gosmann, G., Machado, V., Fiuza, L. M., Moreira, G. R. P. (2012). Effect of Saponin Extracted from Passiflora alata Dryander (Passifloraceae) on development of the Spodoptera frugiperda (J.E. Smith) (Lepidoptera, Noctuidae). International Journal of Plant Research, 2(5): 151-159. http://doi: 10.5923/j.plant.20120205.03
14. Sadek, M. M. (2003). Antifeedant and toxic activity of Adhatoda vascia leaf extract against Spodoptera littoralis (Lep., Noctuidae). Journal of Applied Entomology, 127, 396-404.
15. Adam, R.P. (2001). Identification of Oil Ccomponents by Gas Chromatography/Quadrupole Mass Spactroscopy. Allured Publishing Corporation, Carol Stream, IL, USA.
16. Taghizadeh, Saroukolai, A., Nouri-Ganbalani, G., Rafiee-Dastjerdi, H., Hadian, J. (2014). Antifeedant activity and toxicity of some plant essential oils to Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), Plant Protect. Sci., 50: 207–216.
17. Alaklabi, A., Arif, İ. A., Ahamed, A., Manilal, A., Surendrakumar, R. and Idhayadhulla, A. (2016). Larvicidal, nematicidal, antifeedant and antifungal, antioxidant activities of Mentha spicata (Lamiaceae) root extracts. Tropical Journal of Pharmaceutical Research, 15 (11): 2383-2390. http://dx.doi.org/10.4314/tjpr.v15i11.12
18. Regnault-Roger C., Hamraoui A., Holeman M., Th eron E., Pinel R. (1993). Insecticidal effect of essential oils from Mediterranean plants upon Acanthoscelides obtetus (Say) (Coleoptera Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.). Journal of Chemical Ecology, 14: 1965–1975.
19. Khani, A. and Asghari, J. (2011). Insecticide activity of essential oils of Mentha longifolia, Pulicaria gnaphalodes and Achillea wilhelmsii against two stored product pests, the flour beetle, Tribolium castaneum and the cowpea weevil, Callosobruchus maculates. Journal of Insect Science, 12 (73): 1-10. https://doi: 10.1673/031.012.7301
20. Okut, N., Yagmur, M., Selcuk, N. and Yıldırım, B. (2017).Chemical composition of essential oil of Mentha longifolia L. subsp. longifolia growing wild. Pakistan Journal of Botany, 49(2): 525-529.
21. Baki, H., Aktaş, A. G., Bekircan Ç. ve Bektaş, E. (2017). Antifeedant effects of Ferulago longistylis extracts from Erzincan on Ephestia kuehniella. Biological Diversity and Conservation, 10/1: 110-114.