İnsan eritrosit paketinde kadmiyum ile indüklenen oksidatif strese tannik asit, kakao ve sarı kantaron yağının antioksidatif etkileri

Abstract

Kadmiyum (Cd), çevre kirliliğine neden olan ağır metaller arasında yer almakta ve hücrelerde geçici veya kalıcı oksidatif strese sebep olabilmektedir. Bu toksik maddelerin oluşturduğu oksidatif strese canlı organizmaların oluşturduğu yanıtta antioksidatif enzim mekanizmaları yetersiz kalabilmektedir. Bu çalışmada, Cd ile indüklenen oksidatif stresi önlemek amacıyla, antioksidan etkileri bilinen tannik asit (TA), kakao yağı (KY) ve sarı kantaron yağının (SKY) insan eritrositlerindeki lipit peroksidasyonu ve antioksidan enzimler üzerine olası koruyucu etkileri araştırılmıştır. Bu amaçla yapılan çalışmada, 7 sağlıklı gönüllüden kan örnekleri alınarak eritrosit paketleri hazırlanmıştır. Hazırlanan eritrosit paketlerinden kontrol, Cd, TA, KY, SKY ve kombinasyonlarını (Cd+TA, Cd+KY ve Cd+SKY) içeren deney grupları oluşturulmuştur. Tüm gruplarda süperoksit dismutaz (SOD), malondialdehit (MDA) ve katalaz (KAT) enzim düzeyleri ölçülmüştür. Normal dağılım gösteren ve göstermeyen değişken grupları arasındaki karşılaştırmalar Tek Yönlü Varyans analizi (ANOVA) ve Kruskal-Wallis testi ile değerlendirilmiştir. SOD aktivitesinin Cd grubuna göre (3231±214,6 ü/gHb), Cd uygulaması sonrası TA (3507±168,2 ü/gHb), KY (3518±170,0 ü/gHb) ve SKY (3469±249,5 ü/gHb) uygulanan gruplarda arttığı gözlendi. MDA aktivitesinin Cd grubuna göre (60,3±14,9 nmol/gHb), Cd uygulaması sonrası KY (52,1±24,3 nmol/gHb) ve SKY (54,1±23,7 nmol/gHb) uygulanan gruplarda azaldığı ancak TA (61,5±50,0 nmol/gHb) uygulanan grupta değişmediği gözlendi. KAT aktivitesinin Cd grubuna göre (196,2±223,0 ü/gHb), Cd uygulaması sonrası TA (228,2±31,3 ü/gHb), KY (281,6±295,3 ü/gHb) ve SKY (267,8±69,4 ü/gHb) uygulanan gruplarda arttığı gözlendi. Bu sonuçlar KY ve SKY’nin Cd ile indüklenen oksidatif stresi azaltabileceğini ancak MDA düzeyleri açısından TA’in çok fazla etkili olmadığını göstermektedir. Yapılacak diğer çalışmalar açısından elde edilen bu veriler yol gösterici olabilecektir.

Keywords

• kadmiyum
• oksidatif stres
• insan eritrositi

Antioxidative effects of tannic acid, cacao oil and st. john’s wort oil on the oxidative stress induced by cadmium in packed human erythrocytes

Abstract

Cadmium (Cd) is among the heavy metals causing environmental pollution and can cause temporary or permanent oxidative stress in the cells. Antioxidative enzyme mechanisms may be inadequate in the response of living organisms to oxidative stress caused by these toxic substances. In this study, in order to prevent Cd induced oxidative stress, the possible protective effects of (TA), cacao oil (CO) and St. John’s Wort oil (JWO) on lipid erythrocytes and antioxidant enzymes were investigated. We prepared packed erythrocytes from the blood samples of 7 healthy volunteers. Experiment groups consisting of control, Cd, TA, CO, JWO and their combinations (Cd+TA, Cd+CO ve Cd+JWO) were arranged. In all groups, malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were measured. Comparisons between groups of normally distributed variable were evaluated by One-Way variance analysis (ANOVA). Comparisons between groups of not normally distributed variable wereevaluated by Kruskal-Wallis test. After Cd administration SOD activity was increased in TA (3507± 68.2 u/gHb), CO (3518 ±170.0 u/gHb) and JWO (3469±249.5 u/ gHb) groups compared to Cd group. After Cd administration MDA levels were decreased in CO (52,1±24,3 nmol/gHb) and JWO (54,1±23,7 nmol/gHb) groups compared to Cd group. However, it did not change in the TA (61,5±50,0 nmol/gHb) group. After Cd administration CAT activity was increased in TA (228,2±31,3 u/gHb), CO (281,6±295,3 u/gHb) and JWO (267,8±69,4 u/gHb) groups compared to Cd group (196,2±223,0 u/gHb). These results indicate that HF and SCI may reduce Cd-induced oxidative stress, but TA is not very effective in terms of MDA levels. These data obtained in terms of other studies may be guiding.

Keywords

• cadmium
• oxidative stress
• human erythrocytes

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