Evaluation of antifungal activity of cardamom oil against standard and clinical Candida isolates

Yıl 2018, Cilt: 11 Sayı: 3, 31 - 37, 15.12.2018

İlknur Dağ

Öz

Candida species are the members of the human normal flora and they may cause oppurtunistic fungal infections in immuncompromised patients. The available antifungal treatment regiments is quite limited due to the toxicity, high cost and presence of multidrug resistant strains. Recently, natural essential oils and their constituents have drawn attention for their antimicrobial and antibiofilm efficacies. Cardamom essential oil has strong antimicrobial effects. Cardamom can be isolated from the seeds of the ginger family Zingiberaceae. The aim of this study is to investigate the effects of cardamom essential oil against standard and clinical Candida strains. The minimum inhibitory concentration MIC of cardamom was determined using the broth microdilution method according to the Clinical Laboratory Standards Institute. Amphotericin B was used as a positive standard control antibiotic. Cardamom oil have shown strong antifungal activity the tested all Candida isolates MIC ⩽ 0.1% vol/vol . Our MIC and transmission electron microscopic TEM studies confirms that cardamom oil possess in vitro antifungal activity. It may be used as an alternative antifungal agent or natural food preservatives. In addition it may helpful for the discovery of new antifungal drug. However efficacy, safety and toxicity profiles of this oil will need to be addressed

Anahtar Kelimeler

Candida, cardamom oil, antifungal, TEM, MIC

Kardamom yağının standart ve klinik Candida izolatlarına karşı antifungal etkinliklerinin değerlendirilmesi

Öz

Candida türleri insan normal florasının üyelerinden olup, bağışıklığı baskılanmış hastalarda fırsatçı fungal infeksiyonlara yol açabilmektedirler. Mevcut antifungal tedavi rejimleri toksisite, yüksek maliyet ve çoklu ilaç dirençli suşların bulunuşu sebebiyle bir hayli sınırlıdır. Son yıllarda doğal esansiyal yağlar ve bunların bileşenleri, sahip oldukları antimikrobiyal ve antibiyofilm etkinliklerinden dolayı büyük dikkat çekmektedir. Kardamom esansiyal yağı da güçlü antimikrobiyal etkilere sahiptir ve Zingiberaceae ailesi tohumlarından izole edilebilmektedir. Çalışmamızın amacı standart ve klinik Candida suşlarına karşı bu yağın etkinliğini araştırmaktır. Kardamomun Minimum İnhibisyon Konsantrasyonu MİK , Klinik Laboratuar Standartları Enstitüsü’nün talimatlarına göre sıvı mikrodilüsyon testi ile elde edilmiştir. Çalışmada pozitif standart kontrol olarak Amfoterisin B antibiyotiği kullanılmıştır. Kardamom yağı, test edilen tüm Candida izolatları üzerine güçlü bir antifungal aktivite göstermiştir MİK ⩽ %0.1 vol/vol . MİK ve geçirimli elektron mikroskobik TEM çalışmamız kardamom yağının in vitro antifungal etkinliğini onaylamaktadır. Kardamom yağı alternatif bir antifungal ajan ya da doğal besin koruyucusu olarak kullanılabilir. Ayrıca yeni antifungal ilaçların keşfi için faydalı olabilir. Ancak bu yağın etkinlik, güvenlik ve toksisite profillerinin iyi bir şekilde aydınlatılmasına ihtiyaç bulunmaktadır

Anahtar Kelimeler

Candida, kardamom yağı, antifungal, TEM, MİK

Kaynakça

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