Determination of phenolic compounds and antibacterial activity of Juglans regia

Abstract

Treatment with plants has gained more importance because of reaching critical limits to antibiotic resistant. In this study phenolic compounds of walnut membrane was analyzed using by HPLC. Antibacterial activity of methanol and ethanol extracts of juglans fruit membranes were evaluated against Gram-positive and Gram-negative strains Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Methicillin-Resistant Staphylococcus aureus ATCC 43300, Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PAO1 and clinical Staphylococcus spp. As a results; it was observed that all extracts had antibacterial activity on Gram-positive strains different rate 12 mm to 19 mm . The lowest minimum inhibitory concentration MIC value was detected 1,01 mg/ml for reference strains. HPLC analysis showed walnut membrane extracts have some phenolic acids and flavonoids such as gallic acid, epicatechin, catechin, rutin, p-hydroxy benzoic acid, p-coumaric acid, rosmarinic acid

Keywords

• Walnut membrane,phenolic,HPLC,antibacterial

Juglans regia fenolik bileşen içeriklerinin ve antibakteriyel aktivitesinin belirlenmesi

Abstract

Antibiyotik direncinin kritik seviyelere ulaşması neticesinde enfeksiyon hastalıklarının tedavisinde bitkilerin kullanımı giderek önem kazanmıştır. Yapılan bu çalışmada ceviz ara kabuğunun içerdiği bazı fenolik bileşikler HPLC ile analiz edilmiştir. Ayrıca antibakteriyel etkisi bazı Gram-pozitif ve Gram-negatif Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Methicillin-Resistant Staphylococcus aureus ATCC 43300, Chromobacterium violecium ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PAO1 suşlar ile klinik Stafilokok izolatları üzerinde antibakteriyel etkisi araştırılmıştır. Sonuçta ekstraktların Gram-pozitif bakterilere karşı farklı oranlarda antibakteriyel etkiye sahip olduğu gözlenmiştir 12 mm-19 mm . Gram-negatif bakterilerden ise sadece C. violaceum ATCC 12472 üzerinde antibakteriyel etkisi olduğu gözlenmiştir. Minumum inhibisyon konsantrasyon değeri ise en düşük 1,01 mg/ml olarak tespit edilmiştir. HPLC analiz sonuçlarına göre de farklı oranlarda fenolik asit ve flavonoid gallik asit, epikateşin, kateşin, rutin, p-hidroksi benzoik asit, p-kumarik asit, rosmarik asit içerdiği tespit edilmiştir

Keywords

• Ceviz ara kabuk,fenolik,HPLC,antibakteriyel

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