Antenna and mouthpart defect in Chironomus Camptochironomus tentans larvae Chironomidae and their relevance with habitat characteristics

Abstract

A shallow, eutroph and metal contaminated lake one of the Ramsar sites in Turkey, Lake Uluabat was investigated from August 2004 to July 2005 to determine the defects at some mouthparts mentum, mandible and epipharyngis and antenna of Chironomus Camptochrinomus tentans larvae. Although a total of 1800 chironomid larvae belonging twelve taxa were found in Lake Uluabat, it was dominated by Chironomus Camptochironomus tentans Fabricius, 1805. A total of 327 C. C. tentans were examined, 55.04% of which possessed defects. A total of 12 stations were sampled but all samples of C. C. tentans, collected from two stations where water circulation is reduced, demonstrated the highest incidence of deformities during the study. Defects were found in all mouthparts and antenna in C. C. tentans but mentum and epipharyngis defects were the most frequent. In each sample station, dissolved oxygen, temperature, pH and depth of the lake water were measured as in situ. In addition, concentrations of eight metals Pb, Zn, Cd, Cu, Ag, Cr, Fe and Ni were measured monthly in lake water for all samples at each sampling station, n = 9 and sediment n monthly. = 9 . Analyses of water and sediment from Lake Uluabat showed the presence of metal pollutants such as zinc, nickel, lead and copper. The relationship between defect and water-sediment toxicity relationship was analyzed by Correspondence Analysis. According to the results, the total rate of defect were largely correlated with high amounts of Ni, Zn, Pb and Cd in sediment and PO4, pH in water. In mouthpart; the mentum defects positively correlated with Ag and Zn in sediment, SO4 and COD in water and negatively correlated with Cd in water and Cu in sediment; epipharyngis defect positively correlated with Zn in water and with Ag in sediment. Antenna defects showed a statistically correlation with Cr in sediment and COD, NO3–N, SO4 in water

Keywords

• Defects in Chironomidae larvae,Lake Uluabat

Chironomus Camptochironomus tentans Chironomidae larvaları anten ve ağız parçalarındaki şekil bozuklukları ve habitat özellikleri ile ilişkileri

Abstract

Türkiye’deki önemli Ramsar alanlarından biri olan Uluabat Gölü sığ, ötrof ve metal ile kontamine olmuş bir göldür. Chironomus Camptochrinomus tentans larvalarının anten ve ağıız parçalarındaki mentum, anten, mandibul ve epifarinks şekil bozukluklarını incelemek amacıyla Ağustos 2004-Temmuz 2005 tarihleri arasında örnekler toplanmıştır. Araştırmada toplam 1800 chironomid larvası incelenmiş, Uluabat Gölü’nün 12 Chironomid taxonu içerdiği ve Chironomus Camptochironomus tentans Fabricius, 1805 türünün baskın olduğu tespit edilmiştir. Toplamda 327 C. C. tentans incelenerek bunların %55.04’ünde anten ve ağız parçalarında şekil bozukluklarının olduğu saptanmıştır. Su sirkülâsyonunun azaldığı iki istasyondan toplanan tüm C. C. tentans örnekleri çalışma süresince en yüksek oranda şekil bozukluğunun tespit edildiği örneklerdir. C. C. tentans larvaları tüm ağız parçalarında şekil bozuklukları tespit edilmiş olup, bu normalden sapmaların mentum ve epifarinkste daha yaygın olduğu belirlenmiştir. Her bir örnekleme noktasında, su ölçümleri derinlik, pH, çözünmüş oksijen ve sıcaklık in situ olarak yapılmıştır. Ek olarak, sekiz metalin Cd, Cr, Pb, Cu, Ni, Fe, Zn ve Ag göl suyunda her bir istasyonda bütün örnekler için, n=9 ve sedimentteki n=9 konsantrasyonları aylık olarak analiz edilmiştir. Uluabat Gölü’nden sediment ve su analizleri nikel, çinko, bakır ve kurşun gibi metal kirleticilerin varlığına işaret etmektedir. Anten ve ağız parçalarındaki şekil bozuklukları ile su-sediment toksisitesi arasındaki ilişki Correspondence Analysis Uyum Analizi ile incelenmiştir. Sonuçlarımıza göre anten ve ağız parçalarındaki toplam şekil bozukluğu oranı sedimentte nikel, çinko, kadmiyum ve kurşun, suda ise pH ve fosforun yüksek içerikleri ile pozitif korelasyon göstermektedir. Mentumdaki şekil bzuklukları ise sedimentteki Ag ve Zn, suda SO4 ve COD ile positif korelasyon gösterirken, sudaki Cd ve sedimentteki Cu ile negatif korelasyon göstermektedir. Anten şekil bozuklukları sedimentte krom, suda kimyasal oksijen ihtiyacı, nitrat, sülfat ile pozitif korelasyon göstermektedir. Epifarinks şekil bozuklukları ise suda çinko, sedimentte gümüş ile pozitif korelasyon göstermektedir. Epifarinksteki şekil bozulmaları sudaki Zn ve sedimentteki Ag ile pozitif korelasyon gösterirken, anten yapısındaki bozulmaların sedimentteki Cr ve sudaki COD, NO3–N, SO4 içeriği ile pozitif korelasyon gösterdiği tespit edilmiştir

Keywords

• Uluabat Gölü,Chironomidae larvalarında şekil bozuklukları

References

1. Al-Shami, S., Rawi, C. S. M., Nor, S. A. M., Ahmad, A. H., Ali, A. (2010). Morphological deformities in Chironomus spp. (Diptera: Chironomidae) larvae as a tool for impact assessment of anthropogenic and environmental stresses on three rivers in the Juru River System, Penang, Malaysia. Journal of Environmental Entomology, 39, 210–222.
2. APHA, American Public Health Association. (1992). Standard methods for the examination of water and wastewater. CSS (Complete Statistica System): Statistica. References for Statistical Procedures (DOS and Windows version), Release 3.1. StatSoft Inc., Tulsa (OK): Washington StatSoft Inc.
3. Armitage, P. D., Blackburn, J. H. (1985). Chironomidae in a pennine stream system receiving mine drainage and organic enrichment. Hydrobiologia, 121, 165–172.
4. Arslan, N., Ayık, O., Sahin, Y. (2010a). Diversity and structure of Chironomidae (Diptera) limnofauna of Lake Uluabat, a Ramsar site of Turkey, and their relation to environmental variables. Turkish Journal Fisheries and Aquatic Sciences, 10(3), 235-243.
5. Arslan, N., Koç, B., Cicek, A. (2010b). Metal contents of macroinvertebrates (Oligochaeta - Chironomidae), in water, and bottom sediments of Lake Uluabat, a Ramsar site of Turkey. The Scientific World Journal Environment, 10, 1269-1281.
6. ASTM, American Society for Testing and Materials. (1985). Preparation of biological samples for inorganic chemical analysis. 1. Annual Book of ASTM Standards, D-19: 740–747.
7. Bhattacharyay, G., Sadhu, A. K., Mazumdar, A., Chaudhuri, P. K. (2005). Antennal deformities of chironomid larvae and their use in biomonitoring of heavy metal pollutants in the River Damodar of West Bengal, India. Environmental Monitoring and Assessment, 108, 67–84.
8. Bird, G. A. (1994). Use of chironomid deformities to assess environmental degradation in the Yamaska River, Quebec. Environmental Monitoring and Assessment, 30, 163–175.

9. Camur Elipek, B., Arslan, N., Kırgız, T., Oterler, B., Guher, H., Ozkan, N. (2010). Analysis of benthic macroinvertebrates in relation to environmental variables of Lake Gala (Edirne / Turkey). Turkish Journal of Fisheries and Aquatic Sciences, 10, 315-322.
10. Dalkıran, N., Karacaoglu, D., Dere, S., Senturk, E., Torunoglu, T. (2006). Factors affecting the current status of a eutrophic shallow lake (Lake Uluabat, Turkey): Relationships between water physical and chemical variables. Chemistry and Ecology, 22(4), 279–
11. Diggins, T. P., Stewart, K. M. (1998). Chironomid deformities, benthic community composition, and trace elements in the Buffalo River (New York) area of concern. Journal of the North American Benthological Society, 17(3), 311–323.
12. Eisler R. (1996). Silver hazards to fish, wildlife and invertebrates: A synoptic review. Contaminant Hazard Reviews. Laurel, MD (US): Department of the Interior Patuxent Wildlife Research Center. Report No.:32.
13. Emiroglu, O., Cicek, A., Arslan, N., Aksan, S., Ruzgar, M. (2010). Boron concentration in water, sediment and different organisms around large borate deposits of Turkey. Bulletin of Environmental Contamination and Toxicology, 84(4), 427-431.
14. EPA Method 2007. (2001). Determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectrometry.
15. EPA Method 3051. (1998). Microwave assisted acid digestion of sediments, sludges, soils, and oils.
16. EPA, Environmental Protection Agency (US). (2006). National recommended water quality criteria-correction. Office of Water. 822- z-99-001.
17. Epler, J. H. (2001). Identification manual for the larval Chironomidae (Diptera) of North and South Carolina. A guide to the taxonomy of the midges of the Southeastern United States, including Florida. North Carolina, Special Publication, SJ2001-SP13.
18. Fılık Iscen C., Emiroglu, O., Ilhan, S., Arslan, N., Yılmaz, V. (2008). Application of multivariate statistical techniques in the assessment of surface water quality in Uluabat Lake, Turkey. Environmental Monitoring and Assessment, 144, 269-276.
19. Greenacre, M., Blasius, J. editors. (1994). Correspondence analysis in the social science. San Diego: Academic Press.
20. Hellawell M. J., 1998. Toxic substances in rivers and streams. Environmental Pollution, 50, 61–85.
21. Hudson, L. A., Ciborowski, J. J. (1996). Spatial and taxonomic variation in incidence of mouthpart deformities in midge larvae (Diptera: Chironomidae, Chironomini). Canadian Journal of Fisheries and Aquatic Sciences, 53, 297-304.
22. Janssens de Bisthoven, L. (1999). Biomonitoring with deformities in aquatic organisms. In: Gerhardt A editor. Biomonitoring of Polluted Water. Zürich: Transtech Publications. Environmental Science Forum, 9, 65–94.
23. Janssens de Bisthoven, L., Huysmans, C., Ollevier, F. (1994). The in situ relationships between sediment concentrations of micropollutants and morphological deformities in Chironomus gr. thummi larvae (Diptera, Chironomidae) from lowland rivers (Belgium): a spatial comparison. In: Cranston PA editor. Chironomids: from genus to ecosystems. CSIRO, Melbourne. p. 63-80.
24. Janssens de Bisthoven, L., Nuyts, P., Goddeeris, B., Ollevier, F. (1998). Sublethal parameters in morphologically deformed Chironomus larvae: clues to understanding their bioindicator value. Freshwater Biology, 39, 179-191.
25. Kazancı, N., Leroy, S. A. G., Öncel, S., İleri, Ö., Toprak, Ö., Costa, P., Sayılı, S., Turgut, C., Kibar, M. (2010). Wind control on the accumulation of heavy metals in sediment of Lake Uluabat, Anatolia, Turkey. Project supported by Technical and Scientific Research https://bura.brunel.ac.uk/bitstream/2438/4190/1/JOPL1528R3.pdf. Turkey (YDABCAG 103Y102), and by NATO (EST.CLG.978645).
26. Kokmen, S., Arslan, N., Filik, C., Yılmaz, V. (2007). Zoobenthos of Lake Uluabat, a Ramsar site in Turkey, and their relationship with environmental variables. Clean, 35(3), 266-274.
27. Kosalwat, P., Knight, A. W. (1987). Chronic toxicity of copper to a partial life cycle of the midge Chironomus decorus. Archives of Environmental Contamination and Toxicology, 16, 283–290.
28. Lenat, D. R. (1993). Using mentum deformities of Chironomus larvae to evaluate the effects of toxicity and organic loading in streams. Journal of the North American Benthological Society, 12(3), 265–269.
29. Madden, C. P., Austin, A. D., Suter, P. J. (1995). Pollution monitoring using chironomid larvae: what is a deformity? In: Cranston P editor. Chironomids: from genes to ecosystems. CSIRO Pub East Melbourne, Australia. p. 89–100.
30. Madden, C. P., Suter, P. J., Nicholas, B. C., Austin, A. D. (1992). Deformities in chironomid larvae as indicator of pollution (pesticide) stress. Netherland Journal of Aquatic Ecology, 26, 551–557.
31. Magnin, G., Yarar, M. (1997). Important bird breeding areas in Turkey, İstanbul.
32. Martinez, E. A., Moore, B. C., Schaumloffel, J., Dasgupta, N. (2001). Induction of morphological deformities in Chironomus tentans exposed to zinc- and lead-spiked sediments. Environmental Toxicology and Chemistry, 20(11), 2475–2481.
33. Martinez, E. A., Moore, B. C., Schaumloffel, J., Dasgupta, N. (2004). Teratogenic versus mutagenic abnormalities in chironomid larvae exposed to Zinc and Lead. Archives of Environmental Contamination and Toxicology, 47, 193–198.
34. Ozdamar, K. (1999). Paket programlarla istatistiksel veri analizi “çok değişkenli analizler”. Eskişehir: Kaan Kitabevi.
35. Republic of Turkey Ministry of Environment and Forest. (2015). Regulation on Management of Surface Water Quality.
36. Sahin, Y. (1991). Türkiye Chironomidae Potamofaunası. Project No.:TBAG-869.
37. Simkiss, K., Taylor, M. G. (1989). Metal fluxes across the membranes of aquatic organisms. Critical Reviews in Aquatic Sciences, 1, 173–188.
38. Thornberg, A. E. (1995). Mouthpart deformities in chironomids from heavy metal contaminated sediments of the Coeur d’ Alene region [master’s thesis]. [Pullman (WA)]: Washington State University.
39. Turgut, C. (2005). Ulubat Gölü çökellerinde ve göl suyunda metal konsantrasyonlarının incelenmesi. Yüksek Lisans Tezi, Gebze Yuksek Teknoloji Enstitüsü, Gebze, Kocaeli, 92 p.
40. Uzgoren, N., Uzgoren, E. (2007). Dumlupınar Üniversitesi lisans öğrencilerinin memnuniyetini etkileyen bireysel özelliklerin istatistiksel analizi-hipotez testi, ki-kare testi ve doğrusal olasılık modeli. Dumlupınar Üniversitesi Sosyal Bilimler Dergisi, 17, 173-192.
41. van de Guchte, C., van Urk, G. (1989). Discrepancies in the effects of field and artificially heavy metal contaminated aquatic sediments upon midge larvae. In: Vernet JP editor Heavy metals in the environment. 7th International Conference; Geneva.
42. van Urk, G., Kerkum, F. C. M., Smit, H. (1992). Life cycle patterns, density and frequency of deformities in Chironomus larvae (Diptera: Chironomidae) over a contaminated sediment gradient. Canadian Journal of Fisheries and Aquatic Sciences, 49, 2291– 2299.
43. Vermeulen, V. C. (1995). Elaborating chironomid deformities as bioindication of toxic sediment stress: the potential application of mixture toxicity concepts. Annales Zoologici Fennici, 32, 265-285.
44. Warwick, W. F. (1985). Morphological abnormalities in Chironomidae (Diptera) larvae as measures of toxic stress in freshwater ecosystem: indicating antennal deformities in Chironomus meigen. Canadian Journal of Fisheries and Aquatic Sciences, 42, 1881
45. Warwick, W. F., Tisdale, N. A. (1988). Morphological deformities in Chironomus, Cryptochironomus, and Procladius (Diptera: Chironomidae) from two differentially stressed sites in Tobin Lakes, Saskatchewan. Canadian Journal of Fisheries and Aquatic Sciences, 45, 1123–1144.
46. Yıldız, Z. (2004). Eğitim düzeyi ile suç türü arasındaki ilişkinin araştırılması. Kuram ve Uygulamada Eğitim Yönetimi Dergisi, 38, 278-291.