Influence of surface kind on biofilm formation by Salmonella spp. and Listeria monocytogenes from food-contact surfaces

Yıl 2017, Cilt: 10 Sayı: 1, 124 - 132, 15.04.2017

Bükay Yenice Gürsu İlknur Dağ Şeref İset Gökhan Dıkmen

Öz

Salmonella spp. and Listeria monocytogenes are important pathogenic bacteria, which are transmitted by food. It is known that both microorganisms may produce biofilm on biotic or abiotic surfaces. Bacteria in biofilms exhibit enhanced resistance to cleaning and sanitation. In this study, we investigated the biofilm producing ability of 8 Salmonella spp. and 6 L. monocytogenes isolates by microtiter plate and tube adherence method. All tested Salmonella spp. and L. monocytogenes strains produced biofilm but strains of L. monocytogenes exhibited a higher ability of biofilm formation. Concominantly with these two methods, adhesion and biofilm formation of selected strains to six different industrial surface was also assessed by scanning electron microscope SEM . In addition, biofilm formation and development of selected two strains were also evaluated on granite surfaces and at five incubation periods 2th, 4th, 6th, 24th and 48th hours . Mature biofilm formation was determined after 24 and 48 hours. Granite, marble, wood and glass surfaces presented higher intensity of biofilm, compared to the steel and plastic surfaces. Especially granite and marble are the surfaces in which we found to be the most convenient for the biofilm formation

Anahtar Kelimeler

Biofilm, Salmonella, Listeria, Surface Kind

Besin temas yüzeylerinden elde edilen Salmonella spp. ve Listeria monocytogenes izolatlarının biyofilm oluşumu üzerine yüzey çeşidinin etkisi

Öz

Salmonella spp. ve Listeria monocytogenes besin yoluyla taşınan önemli patojenik bakterilerdendir. Her iki mikroorganizmanın da canlı ve cansız yüzeyler üzerinde biyofilm oluşturabildikleri bilinmektedir. Biyofilmde bulunan bakteriler temizleme ve sanitasyon işlemlerine artan bir direnç gösterirler. Biz bu çalışmada 8 Salmonella spp. ve 6 Listeria monocytogenes izolatının biyofilm oluşturma yeteneğini mikrotitre plaka ve tüp aderans metodlarıyla araştırdık. Test edilen tüm Salmonella spp. ve L. monocytogenes izolatları biyofilm oluşturmuş ancak L. monocytogenes daha yüksek derecede biyofilm oluşumu göstermiştir. Bu iki teste ilave olarak seçilen izolatların altı farklı endüstriyel yüzey üzerindeki adezyon ve biyofilm oluşumları da taramalı elektron mikroskopla tayin edilmiştir SEM . Ayrıca seçilen iki izolatın biyofilm oluşum ve gelişimleri granit yüzeyler üzerinde ve beş farklı inkübasyon periyodunda değerlendirilmiştir 2., 4., 6., 24. ve 48. saatler . Olgun biyofilm oluşumu 24. ve 48. saatler sonrasında tespit edilmiştir. Granit, mermer, tahta ve cam yüzeyler, çelik ve plastik yüzeylere kıyasla daha yoğun biyofilm oluşturmuşlardır. Özellikle granit ve mermer biyofilm oluşumu için daha elverişli yüzeyler olarak bulunmuştur

Anahtar Kelimeler

biyofilm, Salmonella, Listeria, yüzey çeşidi

Kaynakça

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