Brezilya Barbatimão ağacından izole edilen endofitik fungusların uçucu metabolitleri

Yıl 2017, Cilt: 10 Sayı: 2, 1 - 7, 15.08.2017

Gökalp İşcan

Öz

Endofitik funguslar bitkilerle ortaklaşa bir yaşam sürdürmekle birlikte, onların biyotik ve abiyotik stres koşullarına karşı dirençlerini arttırmaktadırlar. Endofitik fungusların biyoçeşitliliği çok fazladır ve birçok biyoaktif bileşik sentezleyebilirler. Bu basit yapılı doğal moleküller ilaç, gıda ve parfümeri endüstrisinin büyük ilgisini çekmektedir. Çalışmamızda Brezilya’nın yerel bir bitkisi olan Stryphodendron adstringens’in yaprak ve gövde kabuklarından 5 farklı endofitik fungus izole edilerek moleküler metotlar ile tanımlanmışlardır. Funguslar uygun sıvı besi ortamı içerisinde kültüre alınarak, ürettikleri uçucu madde profili tepe boşluğu-katı faz mikro-ekstraksiyon ve gaz kromatografisi/kütle spektrometresi GK/KS kullanılarak, ortaya konmuştur. İzole edilen fungusların terpen yapısında mono- ve seskiterpenler ile hidrokarbon yapısında organik uçucu bileşikler ürettikleri saptanmıştır

Anahtar Kelimeler

endofitler, tepe boşluğu-katı faz mikro-ekstraksiyonu, GK/KS, uçucu organik bileşikler

Volatile metabolites of endophytic fungi isolated from Brazilian Barbatimão tree

Öz

Entophytic fungi live in a symbiotic association with higher plants and may improve the host resistance to biotic and/or abiotic stresses. Endophytes have a very rich biodiversity and they can synthesize so many bioactive metabolites. These simple natural molecules are challenging potential resources for the pharmaceutical, food and fragrance industries. In the present study five endophytic fungi were isolated from barks and leaves of Brazilian native tree Stryphodendron adstringens and identified by molecular methods. The fungi were cultured in liquid media and the volatile compounds profile were determined by using headspace solid-phase micro-extraction HS-SPME and gas chromatography/mass spectrometry GC/MS techniques. Hydrocarbones and the terpenic compounds mono- and sesquiterpenes were detected as the main volatiles of the isolated fungus

Anahtar Kelimeler

endophytes, headspace-solid phase micro-extraction, GC/MS, volatile organic compounds

Kaynakça

• Banerjee, D., Strobel, G. A., Booth, B., Sears, J., Spakowicz, D., Busse, S. (2010). An endophytic Myrothecium inundatum producing volatile organic compounds. Mycosphere, 1(3), 241-247.
• Baydar, H., Erbaş, S. (2016). Yağ Gülü (Rosa damascena Mill.)’nde Tepe Boşluğu Katı Faz Mikro Ekstraksiyonu (HS- SPME) ve Konvansiyonel Su Distilasyonu Yöntemleri ile Elde Edilen Uçucu Bileşenlerin Karşılaştırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 20(1), 27-36.
• Buzzini, P., Martini, A., Cappelli, F., Pagnoni, U.M., Davoli, P. (2003). A study on volatile organic compounds (VOCs) produced by tropical ascomycetous yeasts. Antonie Van Leeuwenhoek, 84(4), 301-311.
• Carvalho, C. R., Gonçalves, V. N., Pereira, C. B., Johann, S., Galliza, I. V., Alves, T. M., Rabello, A., Sobral, Zani, C.L., M.E.G., Rosa, C. A., Rosa, L. H. (2012). The diversity, antimicrobial and anticancer activity of endophytic fungi associated with the medicinal plant Stryphnodendron adstringens (Mart.) Coville (Fabaceae) from the Brazilian savannah. Symbiosis, 57(2), 95-107.
• Chaudhary, A., Kaur, P., Singh, B., Pathania, V. (2009). Chemical composition of hydrodistilled and solvent volatiles extracted from woodchips of Himalayan Cedrus: Cedrus deodara (Roxb.) Loud. Natural Product Communications, 4(9), 1257-1260.
• de Capriles, C. H., Mata, S., Middelveen, M. (1989). Preservation of fungi in water (Castellani): 20 years. Mycopathologia, 106(2), 73-79.
• Duan, J. L., Li, X. J., Gao, J. M., Wang, D. S., Yan, Y., Xue, Q. H. (2013). Isolation and identification of endophytic bacteria from root tissues of Salvia miltiorrhiza Bge. and determination of their bioactivities. Annals of Microbiology, 63(4), 1501-1512.
• Ezra, D., Hess, W. M., Strobel, G. A. (2004). New endophytic isolates of Muscodor albus, a volatile-antibiotic- producing fungus. Microbiology, 150(12), 4023-4031.
• Joulain, D., König, W. A. (1998). The atlas of spectral data of sesquiterpene hydrocarbons. Hamburg, EB-Verlag.
• Kompe Özdener, Y., Akın Mutlu, V. (2017). Mycorrhizal diversity in some species of Dactylorhiza genus (Orchidaceae), Biological Diversity and Conservation, 10(1), 55-64.
• Kumar, S., Kaushik, N. (2012). Metabolites of endophytic fungi as novel source of biofungicide: a review. Phytochemistry Reviews, 11(4), 507-522.
• McLafferty, F. W., Stauffer, D. B., Stenhagen, E., Abrahamsson, S., Heller, S. R., Milne, G. W. (1989). The Wiley/NBS Registry of Mass Spectral Data: Vol. 1., New York, John Wiley & Sons.
• Melo J. O., Endo T. H., Bersani-Amado L. E., Svidzinski A. E., Baroni S., Palazzo de Mello J. C., Bersani-Amado C. A. (2007). Effect of Stryphnodendron adstringens (barbatimão) bark on animal models of nociception. Brazilian Journal of Pharmaceutical Sciences, 43(3), 465-469.
• Morath, S. U., Hung, R., Bennett, J. W. (2012). Fungal volatile organic compounds: a review with emphasis on their biotechnological potential. Fungal Biology Reviews, 26(2), 73-83.
• Prenafeta-Boldş, F. X., Andrea, K. U. H. N., Luykx, D. M., Heidrun, A. N. K. E., van Groenestıjn, J. W. (2001). Isolation and characterisation of fungi growing on volatile aromatic hydrocarbons as their sole carbon and energy source. Mycological Research, 105(04), 477-484.
• Rosa, L. H., Vaz, A. B., Caligiorne, R.B., Campolina, S., Rosa, C.A. (2009). Endophytic fungi associated with the Antarctic grass Deschampsia antarctica Desv. (Poaceae). Polar Biology, 32(2), 161-167.
• Roze, L. V., Beaudry, R. M., Linz, J. E. (2012). Analysis of volatile compounds emitted by filamentous fungi using solid-phase microextraction-gas chromatography/mass spectrometry. Fungal Secondary Metabolism: Methods and Protocols, 133-142.
• Schulz, B., Boyle, C. (2005). The endophytic continuum. Mycological research, 109(06), 661-686.
• Sieber, T. N. (2007). Endophytic fungi in forest trees: are they mutualists? Fungal Biology Reviews, 21(2), 75-89.
• Stierle, A., Strobel, G., Stierle, D., Grothaus, P., Bignami, G. (1995). The search for a taxol-producing microorganism among the endophytic fungi of the pacific yew, Taxus brevifolia. Journal Natural Products, 58, 1315–1324
• Strobel A. (2003). Endophytes as sources of bioactive products, Microbes and Infection, 5, 535–544.
• Strobel, G. A. Daisy, B. (2003). Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Reviews. 67, 491–502.
• Strobel, G., Daisy, B., Castillo, U., Harper, J. (2004). Natural products from endophytic microorganisms⊥. Journal of Natural Products, 67(2), 257-268.
• Suryanarayanan, T. S., Thirunavukkarasu, N., Govindarajulu, M. B., Sasse, F., Jansen, R., Murali, T.S. (2009). Fungal endophytes and bioprospecting. Fungal Biology Reviews, 23, 9-19
• Swamy, M. K., Sinniah, U. R. (2015). A Comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.:An Aromatic Medicinal Plant of Industrial Importance. Molecules, 20, 8521-8547.
• Tan, R. X., Zou, W. X. (2001), Endophytes: a rich source of functional metabolites. Natural Product Reports, 18(4), 448-459.
• Ting, A. S. Y., Mah, S. W., Tee, C.S. (2010). Identification of volatile metabolites from fungal endophytes with biocontrol potential towards Fusarium oxysporum F. sp. cubense race 4. American Journal of Agricultural and Biological Sciences, 5(2), 177-182.
• Torres, V.D., Pandey, A., Castro, G.R. (2011). Banana flavor: insights into isoamyl acetate production; In book: Bananas: Nutrition, Diseases and Trade Issues (Cohen, A.E.). Hauppauge, N.Y. Nova Publishers.
• Zhang, H. W., Song, Y. C., Tan, R. X. (2006). Biology and chemistry of endophytes. Natural Product Reports, 23(5), 753-771.