Assesment of genotoxicity induced by lead pollution in tomato Lycopersicum esculentum by molecular and population markers

Yıl 2015, Cilt: 8 Sayı: 1, 83 - 89, 15.04.2015

Semra Soydam Aydın Zeynep Kılıç Çiğdem Dönmez Ersin Altunkaynak Sümer Aras

Öz

Heavy metal contamination is an important environmental problem that may lead to alterations in vital growth processes, mineral nutrition, transpiration, photosynthesis, enzyme activity and nucleic acids. The effects of environmental pollutants on plants can be monitored using various techniques at different levels. Random amplified polymorphic DNA RAPD is a semi-quantitative technique that has been used for genetic mapping, taxonomy, phylogeny and the detection of various kinds of DNA damage and mutations that result from genotoxic agents such as heavy metal contamination. In this study, tomato Lycopersicum esculentum L. seeds that had germinated in various concentrations of Pb NO3 2 solutions were used for measuring population parameters such as dry weight, total soluble protein content, root length and, ultimately, inhibitory rate IR values. The seeds were also used to determine the genotoxic effect of the lead, reflected as the appearance or disappearance of bands in RAPD profiles. Inhibition or activation of root elongation was found to be the first effect of metal toxicity to show up in the plants that were tested. Also, total soluble protein content was significantly affected by increased Pb2+ concentrations. The data obtained from RAPD band profiles and genomic template stability GTS revealed results that were consistent with the population parameters

Anahtar Kelimeler

Tomato Lycopersicum esculentum L., RAPD, lead Pb2+, population parameters

Domates Bitkisinde Lycopersicum esculentum L. Kurşun Kirliliği ile İndüklenen Genotoksisitenin Moleküler ve Populasyon Markörleri ile Değerlendirilmesi

Öz

Ağır metal kirliliği bitkilerin gelişim süreci, mineral beslenme, terleme, fotosentez, enzim aktivitesi ve nükleik asit sentezi gibi bir çok alanda farklılaşmalara sebep olan çevresel bir problemdir. Çevresel kirleticilerin bitkiler üzerindeki etkileri çeşitli teknikler kullanılarak belirlenebilir. Rastgele çoğaltılmış polimorfik DNA RAPD yöntemi genetik haritalama, taksonomi, filogeni ve ağır metal kontaminasyonu gibi genotoksik ajanların neden olduğu çeşitli DNA hasarları veya mutasyonlarının belirlenmesinde kullanılan yarı-nicel bir tekniktir. Bu çalışmada, domates Lycopersicum esculentum L. tohumları farklı konsantrasyonlardaki Pb NO3 2 solüsyonu içerisinde çimlendirilmiş ve bu tohumların kuru ağırlık, toplam çözünebilir protein içeriği, kök uzunluğu ve sonuçta engelleyici oranı IR belirlenmiştir. Tohumlar aynı zamanda kurşun solüsyonunun RAPD profilinde yeni bir band oluşumu veya kayboluşu olarak akseden genotoksik etkisini belirlemek amacıyla kullanılmıştır. Kök uzamasının inhibisyonu veya aktivasyonu bitkilerde metal toksisitesinin ilk belirtisi olarak gözlenmiştir. Aynı zamanda toplam çözünebilir protein içeriği artan Pb2+ konsantrasyonundan önemi oranda etkilenmiştir. RAPD band profillerinden ve genomik kalıp stabilitesinden GTS elde edilen veriler populasyon parametreleri ile uyumludur

Anahtar Kelimeler

Domates Lycopersicum esculentum L., RAPD, kurşun Pb2+, populasyon parametreleri

Kaynakça

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