A tissue culture technique for propagation of Paulownia elongata tree

Year 2013, Volume: 6 Issue: 3, 1 - 5, 15.12.2013

Ely Zayova Maria Petrova Roumiana Vasilevska-ivanova Daniela Stoeva Boris Krapchev

Abstract

Stem tips and nodal explants of Paulownia elongata plants grown at controlled conditions were cultured on Murashige and Skoog medium MS supplemented with NAA and BAP. Medium containing 2 mg/L BAP and 0.05 mg/L NAA gave the best shoot production and maximum number of shoots from nodal explant 8.2 . The results indicated that P. elongata has a great potential for vegetative buds formation as the answer strongly related to the composition of medium and culture conditions. The described protocol allows the establishment of numerous cloned elite plants from selected genotypes of P. еlongatа

Keywords

P. еlongatа, Shoot tips, Nodal explants, Micropropagation, Plant growth regulators

References

• Abou Dahab, A. M., Habib, M.A., Afaf, Y.A. Hosni and A.M.M. Gabr, 2005. Effect of some sterilization treatments and growth regulators on Ruscus hypoglossum L. Arab. J. Biotech. 8/1. 127-140.
• Bergmann, B. A. 1995. Micropropagation of Paulownia elongata. Proc. 23rd South. for Tree Improvement Conf., Asheville, North Carolina, 266.
• Bergmann, B. A., Moon, H. K. 1997. In vitro adventitious shoot production in Paulownia. Plant Cell Rep. 16. 315-319.
• Bergmann, B. A. 1998. Propagation method influences first year field survival and growth of Paulownia. New Forest. 16. 251-264.
• Burger, D. W., Liu, L., Wu, L. 1985. Rapid micropropagation of Paulownia tomentosa. Hort. Sci. 20. 760-761.
• Carpenter, S. B. 1977. This “princess” heals disturbed land. Am. Forests. July. 22-23.
• Chang, S. H., Donald, D. G. M. 1992. In vitro organogenesis and plantlet formation of Paulownia elongata. S. Afr. For. J. 163. 27–29.
• Ipekçi, Z., Altinkut, A., Kazan, K., Bajrovic, K., Gozukirmizi, N. 2001. Highfrequency plant regeneration from nodal explants of Paulownia elongata. Plant Biol. 3. 113–115.
• Ipekçi, Z., Gozukirmizi N. 2004. Indirect somatic embryogenesis and plant regeneration from leaf and internode explants of Paulownia elongata. Plant Cell, Tiss. Org. 79/3. 341-345.
• Kays, J., Johnson, G., Stringer, G. 1997. How to produce and market Paulownia. Bulletin 319, (pp.22), Coop. Exten. Serv., Univ. Maryland.
• Jiang, Z., Gao, L., Fang, Y., Sun, X. 1994. Analysis of Paulownia- intercropping types and their benefits in Woyang County of Anhiu Province. For. Ecol. Manage. 67. 329-337.
• Komamine, A., Kawara, R., Matsumoto, M., Sunabori, S., Toya, T., Fujimura, T. 1992. Mechanisms of somatic embryogenesis in cell cultures: physiology, biochemistry and molecular biology. In Vitro Cell & Dev. Biol. 28. 11- 14.
• Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15. 475–497.
• Olson, J. R., Carpenter, S. B. 1985. Specific gravity, fiber length, and extractive content of young Paulownia. Wood and Fiber Sci. 17/14. 428-438.
• Ozaslan, М., Can, C., Aytekin, T. 2005. Effect of explant source on in vitro propagation of Paulownia tomentosa Steud. Biotechnol. & Biotechnol. Eq. 19/3. 20-26.
• Rao, D. C., Goh, C. J., Kumar, P. P. 1996. High frequency adventitious shoot regeneration of Paulownia spp. cultured in vitro. Plant Cell Rep. 16. 204-209.
• Sayed, S. S., Gabr, A. M. M. 2007. In vitro culture and genetic stability of Deutzia scabra THVNB. J. Biol. Chem. Environ. Sci. 2/2. 321-336.
• Slavova, J. 1988. Development of in vitro methods for vegetative propagation and haploids production in Beta vulgaris. PhD Thesis, Shumen (in Bulgarian).
• Slavova, J., Kaschieva, M. 2004. Implementation of in vitro method for obtaining fodder beet haploid plants (Beta vulgaris L var Crassa) I. Obtaining of shoots from isolated unpollinated ovules of fodder beet. Field Crops Studies, 1/2. 273 – 277.
• Song, S. L., Sato, T., Ishii, K., Saito, A., Obba, K. 1990. In vitro mass propagation by meristem culture of two mature trees of Paulownia catalpifolia. J. Jpn. For. Sci. 72/6. 495-498.
• Upadhyay, R., Arumugam, N., Bhojwani, S. S. 1989. In vitro propagation of Picrorhiza kurroa Royle Ex. Benth.: an endangered species of medicinal importance. Phytomorphology 39/2, 3. 235–242.
• Wang, Q., Shogren, J. F. 1992. Characteristics of the crop Paulownia system in China. Agriculture, Ecosystems and Environ. 39. 145–152.
• Yang J. C., Ho C. K., Chen Z. Z., Chang S. H. 1996. Characteristics of the crop –Paulownis system in China. Agriculture, Ecosystems and Environ., 39. 145-152.
• Zayova, E., Vassilevska-Ivanova, R., Kraptchev, B., Stoeva, D. 2010. Somaclonal variations through indirect organogenesis in eggplant (Solanum melongena L.). BioDiCon. 3/3. 1-5.
• Zhu, Z. H., Chao, C. J., Lu, X. Y. and Xiong, Y. G. 1986. Paulownia in China: Cultivation and utilization. Asian Network for Bio Sci. & Intern. Devel. Res. Centre, Canada. pp. 65.. .