Pharmacognostic value of leaf anatomy and trichome morphology for identification of forskolin in a novel medicinal plant Coleus forskohlii

Yıl 2011, Cilt: 4 Sayı: 2, 165 - 171, 15.08.2011

Selima Khatun Ugur Cakılcıoglu Narayan C Chatterjee

Öz

Forskolin, a labdane diterpene is the active principle of the indigenous medicinal plant Coleus forskohlii Briq. It is the only known natural source of the diterpenoid forskolin. Forskolin has a unique property of activating almost all hormone sensitive adenylate cyclase enzymes in a biological system. The pharmacological and biochemical investigations established that forskolin possesses multifaceted biological activities. This research paper have highlighted on phytochemical vis-a-vis histochemical localization of other terpenoids and forskolin in leaves of C. forskohlii. Qualitative analysis showed that terpenoids were present in leaves of the herb. Histochemical analysis of leaves of C. forskohlii shows that forskolin was found in the cells of palisade parenchyma, spongy parenchyma, and glandular trichomes of leaf in both the upper and lower epidermis. Thin layer chromatography of chloral hydrate washings showed presence of other terpenoids and forskolin in leaves of C. forskohlii. Rf value of forskolin was 0.6 and Rf values of other terpenoids were 0.62, 0.66, 0.86. This confirms that the yellowish-violet coloured vesicles seen in the sections of leaf contain the terpenoids. The leaves of the Indian drug plant are needed very badly to identify, isolate, design, develop, modify or to prepare new pharmacologically active compounds from the other terpenoids than forskolin. The mechanisms of action of various secondary metabolites isolated from this potential medicinal herb are yet to be elucidated. Leaves are significant p

Anahtar Kelimeler

Coleus forskohlii, Forskolin, Phytochemical analysis, Leaf anatomy, Trichome morphology

Yeni keşfedilen Coleus forskohlii tıbbi bitkisinde forskolinin teşhisi için yaprak anatomisi ve tüy morfolojisinin farmakognozik değeri

Öz

Labdan yapılı diterpen olan forskolin, doğal olarak yetişen tıbbi bir bitki olan Coleus forskohlii Briq.’in etken maddesidir. Bilinen tek doğal diterpenoid forskolin kaynağıdır. Forskolin, bir biyolojik sistemdeki hemen hemen bütün hormona duyarlı adenilat siklaz enziminlerini aktive etme özelliğine sahiptir. Farmakolojik ve biyokimyasal çalışmalar forskolinin çok yönlü biyolojik aktivitelere sahip olduğunu göstermiştir. Bu çalışmada C. forskohlii yapraklarında diğer terpenoidlerin ve forskolinin histokimyasal ve fitokimyasal lokalizasyonu karşılıklı olarak açıklanmıştır. Yapılan nitel araştırma, bitkinin yapraklarında terpenoid bulunduğunu göstermiştir. C. forskohlii bitkisinin yaprakları üzerinde yapılan histokimyasal inceleme palizat parenkiması, sünger parenkiması hücrelerinde ve hem üst hem alt epidermisteki glandüler tüylerde forskolin bulunduğunu göstermiştir. Kloral hidratla yıkanan materyallerin ince tabaka kromatografisi C. forskohlii yapraklarında forskolin ve diğer terpenoidlerin bulunduğunu ortaya koymuştur. Forskolin için Rf değeri 0.6; diğer terpenoidler için Rf değerler ise 0.62, 0.66, 0.86 olarak bulunmuştur. Bu sonuç, yaprak seksiyonlarında görülen sarımsı-mor renkli keseciklerin terpenoid içerdiğini doğrulamaktadır. Forskolin dışında diğer terpenoidlerden farmakolojik olarak aktif bileşiklerin teşhis edilmesi, izole edilmesi, tasarlanması, geliştirilmesi, değiştirilmesi veya hazırlanması için Hindistan da tıbbi bitki yapraklarına büyük ölçüde ihtiyaç duyulmaktadır. Bu potansiyel tıbbi bitkiden izole edilen çeşitli sekonder metabolitlerin etki mekanizmaları henüz açıklığa kavuşmamıştır. Kök ve yumrulara kıyasla yapraklar önemli ölçüde daha zengin antioksidan kaynağıdır p

Anahtar Kelimeler

Coleus forskohlii, Forskolin, Fitokimyasal analizi, Yaprak anatomisi, Tüy morfolojisi

Kaynakça

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