Çimento tozu kirliliğine maruz kalan bitkilerde RAPD analizi ile genomik stabilite ve DNA hasarının analizi

Year 2019, Volume: 12 Issue: 3, 65 - 69, 15.12.2019

Serap Sunar , Filiz Aygun Erturk Guleray Agar

Abstract

En önemli çevre kirleticilerinden biri çimento tozudur. Bu çalışmada, çimento tozunun bitkiler üzerindeki potansiyel genotoksik etkisini tanımlamak için RAPD teknolojisi kullanılmıştır. Bu amaçla, Erzurum'da Askale çimento fabrikasına 10000 m (Kontrol alanı) ve 0-100 m uzaklıkta toplanan 12 bitki türünün ( Convolvulus sepium, Astragalus christianus, Taraxacum androssovii Medicago varia, Alyssum murale, Artemisia spisigera, Falcaria vulgaris, Anchusa strigose, Glaucium leiocarpum, Salvia syriaca, Cryciata taurica Tragopogon albinervis)DNA karşılaştırması yapıldı. Çalışmada 16 RAPD primeri kullanılmış ve 390 bant elde edilmiştir. 0-100m alanlardan toplanan bitki RAPD profillerinde, kontrollerine göre (fabrikadan 10000 metre uzakta) önemli farklılıklar gözlenmiştir. Kontrol bitkilerinde bazı bantların kaybolduğu ve yeni bantların oluştuğu görülmüştür. Ayrıca, GTS (Genomic template stability) değeri, 0-100 m alandan toplanan bitkilerde, kontrollerinkilerle karşılaştırıldığında önemli ölçüde azaldığı görülmüştür( %56.20). 0-100m ve kontrol alanından toprak örnekleri toplanmış ve ağır metal içerikleri bakımından incelenmiştir. Analiz sonuçlarına göre, 0-100 m'den toplanan topraklarda Nikel, Kadmiyum, Çinko, Kurşun ve Bakır konsantrasyonlarının, çarpıcı şekilde yüksek olduğu bulunmuştur. Bu sonuçlar topraktaki yüksek ağır metal içeriğinin bitkilerde RAPD profillerinde ve GTS’de değişikliklere neden olduğunu göstermektedir. Sonuçlar RAPD ve GTS testlerinin çevre kirliliğinin bitkiler üzerindeki genotoksik etkisini tanımlamak için temel, etkili ve tekrarlanabilir yöntemler olduğunu göstermiştir.

Keywords

çimento tozu, bitki, toprak, ağırmetal, genotoksisite

Analysis of genomic stability and DNA damage in plants exposed to cement dust pollution using the RAPD analysis

Abstract

One of the most important environmental pollutants is cement dust. In this study, RAPD technology was used to define the potential genotoxic impact of cement dust on plants. For this purpose, the DNA comparison of 12 plant species( Convolvulus sepium, Astragalus christianus, Taraxacum androssovii Medicago varia, Alyssum murale, Artemisia spisigera, Falcaria vulgaris, Anchusa strigose, Glaucium leiocarpum, Salvia syriaca, Cryciata taurica Tragopogon albinervis)collected from the areas of 10000 m (Control area) or 0-100 m away from Askale cement factory in Erzurum; was performed. 16 primers were used, and 390 bands were obtained. Important differences were observed in plant RAPD profiles collected from 0-100 m areas when compared to their respective controls (10000 m away from the factory). Some bands in control plants got lost, and new band formations were observed. The Genomic template stability (GTS) value decreased significantly in plants collected from 0-100 m area as compared to the controls (%56.20). Soil samples from 0-100 m and control area were collected and examined for their heavy metal contents. According to the analysis results, Nickel, Cadmium, Zinc, Lead and Copper concentrations were found to be dramatically high in the soil collected from 0-100 m as compared to the control area. These results indicate that the high heavy metal content in the soil causes the changes in RAPD profiles and GTS in plants. The data also indicated that RAPD and GTS assays are basic, effective and reproducible means of the genotoxicity control of environmental pollution.

Keywords

Cement dust, Plants, Soil, Heavy Metals, Genotoxicity

References

• Isıklı, B., Demir, T.A., Akar, T., Berber, A., Urer, S.M. et al. (2006). Cadmium exposure from the cement dust emissions: A field study in a rural residence. Chemosphere, 63, 1546-1552.
• Ade-Ademilua, O.E., Umebese, C.E. (2007). The growth of Phaseolus vulgaris L. cv. Ife Brown (Leguminosae) in a cement site rich in heavy metals. Pakistan Journal of Biological Sciences, 10,182-185.
• Uysal,I.,Ozdilek,H.G.,Ozturk,M. 2012. Effect of kiln dust from a cement factory on growth of Vicia faba L. J. Environ. Biol., 33, NO. 02 (Suppl.) pp: 525-530.
• Ozturk, M., Ashraf, M., Aksoy,A.,Ahmad, M.S. A. (Eds.) 2015. Plants, Pollutants & Remediation. Springer Science+Business Media, NY, 407 pp.
• Gupta, A.K., Mishra, R.M. (1994). Effect of lime kilns air pollution on some plant species. Environmental Science and Pollution Research,13, 1-9.
• Iqbal, M.Z., Shafug, M. (2001). Periodical Effect of Cement Dust Pollution on the Growth of Some Plant Species. Turkısh Journal of Botany, 25,19-24.
• Sheikh, K.H., Ozturk, M., 1976. Secmen, O., Vardar, Y., Field studies of the effects of cement dust on the growth and yield of olive trees in Turkey. Environmental Conservation, 3, 117-121.
• Cortez Pimental, J., Peixoto Menezes, A. (1978). Pulmonary and hepatic granulomatous disorders due to the inhalation of cement and micadust. Thorax, 33:219–227.
• Malceva, L.M., Tatanov, L.A. (1974). Professional ‘nye zabolevanija organov dyhanija V uslovijah sovremennogo cementogo proizvodstva. Gigiena Trudai professional ‘nye zabolevanija, 3:14.
• Rafnsson, V. and Johannesdottir, S.G. (1986). Mortality among masons in Iceland. British Journal of Industrial Medicine, 43, 522–525.
• Albin, M, Jakobsson, K., Attewell, R., Johansson, L., Wellinder, H. (1990). Mortality and cancer morbidity in cohorts of asbestos cement workers and referents. British Journal of Industrial Medicine 47:602–610.
• Jakobsson, K., Attewell, R., Hultgren, B., Sjoland, K. (1990). Gastrointestinal cancer among cement workers — a case referent study. International Archives of Occupational and Environmental Health (Int Arch Occup Environ Health), 62, 337–340.
• Jakobsson, K., Horstmann, V., Welinder, H. (1993). Mortality and cancer morbidity among cement workers. The British Journal of Industrial Medicine, 50,264–272.
• Fatima, S.K., Aruna Prabhavathi, P., Padmavathi, P., Reddy, P.P. (2001). Analysis of chromosomal aberrations in men occupationally exposed to cement dust, Mutation Research 490,179–186
• Zeinalzadehtabrizi, H., Hosseinpour, A., Aydin, M., Haliloglu, K. (2015). A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes, Journal of Biodiversity and Environmental Sciences, 7, 222-225.
• Mutlu, S., Atıcı, O., Kaya, Y. (2009). Effect of cement dust on diversity and antioxidant enzyme activities of plants growing around a cement factory. Fresenius Environmental Bulletin,18(10),1823-1827.
• Sharma, Y.K., Davis, K.R. (1997). The effects of ozone on antioxidant responses in plants. Free Radical Biology and Medicine, 23:480-488.
• Alscher, R.G., Erturk, N., Heath, L.S. (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants, Journal Of Experimental Botany, 53,1331-1341.
• Verma, S., Dubey, R.S. (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants, Plant Science, 164,645-655.
• Ahmad P., Ozturk, M., Gucel, S. (2012). Oxidative damage and antioxidants induced by heavy metal stress in two cultivars of mustard (Brassica juncea L.) plants. Fresenius Environmental Bulletin, 21 (10), 2953-2961.
• Erturk, F., Agar, G., Arslan, E., Nardemir, G., Sahin, Z. (2014). Determination of genomic instability and DNA methylation effects of Cr on Maize (Zea mays L.) using RAPD and CRED-RA analysis. Acta Physıologıae Plantarum, 36(6),1529-1537.
• Taspinar, M.S., Agar, G., Yildirim, N., Sunar, S., Aksakal, O., Bozari, S. (2009). Evaluation of selenium effect on cadmium genotoxicity in Vicia faba using RAPD. Journal of Food, Agriculture and Environment, 7(4), 857-860.
• Samardakiewicz, S., Wozny, A. (2005). Cell division in Lemna minor roots treated with lead. Aquatic Botany, 83,289-295.
• Ghori, N.H., Ghori, T., Hayat, M.Q., Imadi, S.R., Gul,A., Altay,V., Ozturk,M. (2019). Heavy metal stress and responses in plants. International Journal of Environmental Science and Technology, 16, 1807–1828.
• Gichner, T., Znidar, I., Szakova, J. (2008). Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants. Mutation Research, 652, 186-190.
• Yücel, E., Hatipoğlu, A., Güner, Ş.T. (2008). The Effects of the Lead (PbCl2) on Mitotic Cell Division of Anatolian Black Pine (Pinus nigra ssp. pallasiana). Biological Diversity and Conservation 1(2), 124-129.