Türkiye'de doğal olarak yetişen Nepeta isaurica Boiss. Heldr. apud Bentham and Nepeta sulfuriflora P.H. Davis'in uçucu yağ bileşimi

Year 2023, Volume: 16 Issue: 1, 51 - 55, 15.04.2023

Hasan Maral , Ömer Çeçen , Musa Türkmen

https://doi.org/10.46309/biodicon.2023.1219905

Abstract

Nepeta türleri halk hekimliğinde terletici, idrar söktürücü, antiseptik, büzücü, astım önleyici, öksürük kesici, spazm çözücü, ateş düşürücü, yatıştırıcı ve mide söktürücü olarak yaygın şekilde kullanılmaktadır. Bu çalışma, Nepeta isaurica ve Nepeta sulfuriflora bitkilerinin uçucu yağının kimyasal bileşimini belirlemek için planlanmıştır. N. isaurica bitkisinin toprak üstü kısımlarının %0,72 oranında uçucu yağ içerdiği, GC-MS analizinde toplam yağın %98,86'sını temsil eden 37 bileşik tespit edilmiş ve uçucu yağın ana bileşenlerinin trans-pinokarveol, α-pinen, linalool, 1,8-sineol, α-terpineol, α-cadinol, spathulenol ve β-pinen olduğu belirlenmiştir. N. sulfuriflora'nın toprak üstü kısımlarının %0.64 oranında uçucu yağ içerdiği belirlenmiştir. GC-MS analizinde ise toplam yağın %99.07'sini temsil eden 37 bileşik tanımlanmıştır. N.sulfuriflora'da bulunan uçucu yağın ana bileşeninin 1,8-sineol (%72,05) olduğu belirlenmiştir. Çalışma sonucunda N. sulfuriflora'nın 1,8-sineol deposu olarak kullanılabileceği sonucuna varılmıştır.

Keywords

uçucu yağ, GC-MS, Lamiaceae, Nepeta isaurica, Nepeta sulfuriflora

Essential oil composition of Nepeta isaurica Boiss. Heldr. apud Bentham and Nepeta sulfuriflora P.H. Davis growing naturally in Türkiye

Abstract

Nepeta species are widely used in folk medicine as a diaphoretic, diuretic, antiseptic, astringent, anti-asthmatic, cough suppressant, antispasmodic, antipyretic, sedative and stomachic. This study was designed to examine the chemical composition of the essential oil of Nepeta isaurica and Nepeta sulfuriflora. It was determined that the aerial parts of the N. isaurica plant contained 0.72% essential oil, 37 compounds were identified representing 98.86% of the total oil analyzed via GC-MS. The major components of the essential oil obtained from these plants were trans-pinocarveol, α-pinene, linalool, 1,8-cineole, α-terpineol, α-cadinol, spathulenol and β-pinene. It was determined that the aerial parts of N. sulfuriflora contained 0.64% essential oil. GC-MS analysis identified 37 compounds representing 99.07% of the total oil. It has been determined that the main component of the essential oil in N. sulfuriflora is 1,8-cineol (72.05%). As a result of the study, it was concluded that N. sulfuriflora could be used as a 1,8-cineol reservoir.

Keywords

uçucu yağ, GC-MS, Lamiaceae, Nepeta isaurica, Nepeta sulfuriflora

References

• [1] Cardona, J., Celis, A., Cuca, L., Delgado, W., et al. (2008). Extractos vegetales utilizados como biocontroladores conenfasis enla familia Piperacea. Una Revision Agronomia Colombiana, 26, 97-106.
• [2] Sharma, A. & Sing Cannoo, D. (2013). Phytochemical composition of essential oils isolated from different species of genus Nepeta of Labiatae family: A Review. Pharmacophore, 4 (6), 181-211.
• [3] Baytop, T. (1984). Türkiye’de Bitkiler ile Tedavi. (Vol 40, pp. 240-376). Istanbul Üniv. Eczacılık Fakültesi Yayınları.
• [4] Asparganah, J., Sarabian, S. & Ziarati, P. (2013). Essential oil of Nepeta genus (Lamiaceae) from Iran: a review. J Essent Oil Res., 26 (1), 1-12.
• [5] Kharazian, N., Shourabi, S. Z. & Yousefi, M. (2013). Chromosome count and study of eleven Nepeta L. (Lamiaceae) species from Iran. Biodicon, 6 (1), 76-87.
• [6] Hedge, I. C. & Lamond J. M. (1982). Nepeta L. In P. H. Davis, Flora of Turkey and East Aegean Islands. (Vol 7, pp. 264-288). Edinburgh: Edinburgh University Press.
• [7] Ayla, K. & Dirmenci, T. (2008). Nutlet surface micromorphology of the Genus Nepeta L. (Lamiaceae) in Turkey. Turkish Journal of Botany, 32 (2), 103-112.
• [8] Sarıkürkçü, C., Eskici, M., Karanfil, A. & Tepe, B. (2019). Phenolic profile, enzyme inhibitory and antioxidant activities of two endemic Nepeta species: Nepeta nuda subsp. glandulifera and N. Cadmea. South African Journal of Botany, 120, 298-301.
• [9] Maral, H., Kayıcılar, F. R., Cecen, O., Turkmen, M. & Turk Baydır, A. (2022). Antioxidant activity and chemical composition of the essential oil of Nepeta cilicica Boiss. Apud Bentham from Turkey. 3rd International Conference on Applied Engineering and Natural Sciences (ICAENS 2022) July 20-23, 2022, Konya.
• [10] Türkmen, M., Kara, M., Maral, H. & Soylu, S. (2022). Determination of chemical component of essential oil of Origanum dubium plants grown at different altitudes and antifungal activity against Sclerotinia sclerotiorum. Food Process Preserv. 2022;46: e15787. doi: 10.1111/jfpp.15787.
• [11] Harborne, J. B. & Baxter, H. (2001). Chemical dictionary of economic plants, John Wiley & Sons, 224 p. [12] Dorsam, B., WU, C. F., Efferth, T., Kaina, B. & Fahrer, J. (2015). The eucalyptus oil ingredient 1,8-cineol induces oxidation DNA damage. Arch Toxicol, 89 (5), 797-805.
• [13] Klocke, J. A., Darlington, M. V. & Balandrin, M. F. (1987). 1,8- Cineole (Eucalyptol), a mosquito feeding and ovipositional repellent from volatile oil of Hemizonia fitchii (Asteraceae). J Chem Ecol, 13 (12), 2131- 2141.
• [14] Sfara, V., Zerba, E. N. & Alzogaray, R. A. (2009). Fumigant insecticidal activity and repellent effect of and essential oils and seandn monoterpenes on first-instar nymphs of Rhodnius prolixus. J Med Entomol, 46 (3), 511-515.
• [15] Loi, R. X., Solar, M. C. & Weidenhamer, J. D. (2008). Solid-phase microextraction method for in vivo measurement of allelochemical uptake. J Chem Ecol, 34 (1), 70-75.
• [16] Baser, K. H. C., Demircakmak, B. & Duman, H. (1997). Composition of the essential oils of Nepeta flavida Hub. -Mor and Nepeta sulfuriflora P.H. Davis. Proceedings of the XIth Symposium on Plant Originated Crude Drugs. Ankara, May 22-24, 450-456.
• [17] Baser, K. H. C., Kirimer, N., Kurkcuoglu, M. & Demirci, B. (2000). Essential oil of Nepeta species growing in Turkey. Chemistry of Natural Compounds, 36 (4): 356-359.
• [18] Kökdil, G., Kurucu, S. & Topçu, G. (1997). Investigation on the essential oil of some Nepeta species growing in South Anatolia. Proceedings of the XIth Symposium on Plant Originated Crude Drugs. Ankara, May 22-24, 446-449. [19] Dehghan, G., Torbati, S., Mohammadian, R., Movafeghi A. & Talebpour, A.H. (2018). Essential oil composition, total phenol and flavonoid contents and antioxidant activity of Salvia sahendica at different developmental stages,” Journal of Essential Oil Bearing Plants, 21 (4), 1030-1040.
• [20] Javazi, H., Saadatmand, S., Delazar, A. & Khavari Nejad, R.A. (2016). Influence of different developmental stages on content and composition of the essential oil and antioxidant activity of Nepeta scrophularioides Rech.f. essential oil. Journal of Essential Oil Bearing Plants, 19, 693-698.
• [21] Sellami, I. H., Maamouri, E., Chahed, T., Wannes, W. A., Kchouk, M. E. & Marzouk, B. (2009). Effect of growth stage on the content and composition of the essential oil and phenolic fraction of sweet marjoram (Origanum majorana L.). Industrial Crops and Products, 30, 395-402.
• [22] Maksimovic, M., Vidic, D., Miloš, M., Edita Šolic, M., Abadic, S. & Siljak-Yakovlev, S. (2007). Effect of the environmental conditions on essential oil profile in two Dinaric Salvia species: S.brachyodon vandas and S. officinalis L. Biochemical Systematics and Ecology, 35, 473-478.
• [23] Lattoo, S. K., Dhar, R. S., Dhar, A. K., Sharma, P. R. & Agarwal, S. G. (2006). Dynamics of essential oil biosynthesis in relation to inflorescence and glandular ontogeny in Salvia sclarea. Flavour and Fragrance Journal, 21, 817-821.
• [24] Oliveira, M. J., Campos, I. F. P., Oliveira, C. B. A., Santos, M. R., Souza, P. S., Santos S. C., Seraphin, J. C. & Ferri, P. H. (2005). Influence of growth phase on the essential oil composition of Hyptis suaveolens. Biochemical Syst. and Ecol., 33, 275-285.
• [25] Thappa, R. K., Agarwal, S. G., Srivastava, T. N. & Kapahi, B. K. (2001). Essential oils of four Himalayan Nepeta species. J Essent Oil Res. 13:189–191.
• [26] Kashyap, T. K., Melkani, A.B., Mathela, C. S., Dev, V., Olmstead, M. M., Hope, H. & Bottini, A. T. (2003). Essential oil from Nepeta govaniana Benth. study of its major terpenes. J Essent Oil Res. 15:28–30.
• [27] Alim, A., Goze, I., Cetin, A., Atlas, A. D., Cetinus, S.A. & Vural, N. 2009. Chemical composition and in vitro antimicrobial and antioxidant activities of the essential oil Nepeta nuda L. subsp. albiflora (Boiss.) gams. Afr J Microbiol Res. 3:463–467.
• [28] Bisht, D. S., Padalia, R. C., Singh, L., Pande, V., Lal, P. & Mathela, C. S. (2010). Constituents and antimicrobial activity of the essential oils of six Himalayan Nepeta species. J Serb Chem Soc. 75:739–747.
• [29] Shafaghat, A. & Oji, K. (2010). Nepetalactone content & antibacterial activity of essential oils from different parts of Nepeta persica. Nat Prod Commun. 5:625–628.
• [30] Bisht, D. S., Joshi, S. C., Padalia, R. C. & Mathela, C. S. (2012). Isoiridomyrmecin rich essential oil from Nepeta erecta Benth. and its antioxidant activity. Nat Prod Res. 26:29–35.
• [31] Rather, M. A., Hassan, T., Dar, B. A., Shawl, A. S., Qurishi, M. A. & Ganai, B. A. (2012). Essential oil composition of Nepeta raphanorhiza Benth growing in Kashmir valley. Rec Nat Prod. 6:67–70.
• [32] Soltanbeigi, A. & Sakartepe, E. (2020). Chemical specification of wild Salvia tomentosa Mill. collected from Inner Aegean Region of Turkey. Z Arznei Gewurzpfla 24(1): 31-35.
• [33] Soltanbeigi, A. & Maral, H. (2022). Agronomic yield and essential oil properties of Purple Coneflower (Echinacea purpurea L. Moench) with different nutrient applications. Chilean J. Agric. Anim. Sci., ex Agro-Ciencia, 38 (2): 164-175.