Isolation and molecular characterization of non-Azotobacter bacteria using Azotobacter isolation protocols from pastures in the south of Turkey

Yıl 2023, Cilt: 16 Sayı: 3, 252 - 257, 15.12.2023

Ebru Çelen

https://doi.org/10.46309/biodicon.2023.1273996

Öz

Isolation and molecular characterization of non-Azotobacter bacteria using Azotobacter isolation protocols from pastures in the south of Turkey

Ebru ÇELEN
ORCID: 0000-0002-8452-5933

Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, Gölköy, 14280 Bolu, Türkiye

Abstract
Soil is a biodiversity-rich ecosystem. Nitrogen-fixing Azotobacter bacteria, an important component of this ecosystem, are frequently isolated using various methods. The aim of the present study was to perform partial molecular characterization of non-Azotobacter isolates derived during two Azotobacter isolation protocols, as well as to determine which bacteria can be obtained using this method. A total of 800 isolates were acquired from soil samples collected from various pastures in Antalya province of Turkey, with 92 of them being molecularly characterized. These isolates were clustered through RFLP analysis of 16S rRNA gene and the DNA sequences of the isolates representing different groups were performed. According to these results, the bacteria belonging to various genera (Agrobacterium, Phyllobacterium, Variovorax, Acinetobacter, Pseudomonas, Agromyces, and Arthrobacter) were identified. The results show that similar bacteria could be obtained through two isolation protocols used in this study. However, more diverse bacteria were encountered on the Brown-medium than on soil-past.
Keywords: Azotobacter, isolate, soil, 16S rDNA

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Anahtar Kelimeler

Azotobacter, isolate, soil, 16S rDNA

Türkiye'nin güneyindeki meralardan Azotobacter izolasyon protokolleri kullanılarak Azotobacter olmayan bakterilerin izolasyonu ve moleküler karakterizasyonu

Öz

Türkiye'nin güneyindeki meralardan Azotobacter izolasyon protokolleri kullanılarak Azotobacter olmayan bakterilerin izolasyonu ve moleküler karakterizasyonu
Özet
Toprak, biyolojik çeşitlilik açısından zengin bir ekosistemdir. Bu ekosistemin önemli bir bileşeni olan azot bağlayan Azotobacter bakterileri çeşitli yöntemlerle sıklıkla izole edilir. Bu çalışmanın amacı, iki Azotobacter izolasyon protokolü sırasında elde edilen Azotobacter olmayan izolatların kısmi moleküler karakterizasyonunu gerçekleştirmek ve bu yöntemle hangi bakterilerin elde edilebileceğini belirlemektir. Türkiye'nin Antalya ilindeki çeşitli meralardan toplanan toprak örneklerinden toplam 800 izolat elde edilmiş ve bunlardan 92'si moleküler olarak karakterize edilmiştir. İzolatlar, 16S rRNA genlerinin RFLP analizi ile gruplandırılmış ve farklı grupları temsil eden izolatların DNA dizileri gerçekleştirilmiştir. Bu sonuçlara göre çeşitli cinslere (Agrobacterium, Phyllobacterium, Variovorax, Acinetobacter, Pseudomonas, Agromyces ve Arthrobacter) ait bakteriler tespit edilmiştir. Sonuçlar, bu çalışmada kullanılan iki izolasyon protokolü ile benzer bakterilerin elde edilebileceğini göstermektedir. Bununla birlikte, Brown Besi ortamında Soil-Past’a göre daha çeşitli bakteriler tespit edilmiştir.
Anahtar kelimeler: Azotobacter, izolat, toprak, 16S rDNA

Anahtar Kelimeler

Azotobacter, izolat, toprak, 16S rDNA

Destekleyen Kurum

Akdeniz Üniversitesi ve York Üniversitesi

Teşekkür

Çalışmaya konu olan bakteriler Akdeniz Üniversitesi Fen-Edebiyat Fakültesi Biyoloji Bölümü Laboratuvarlarında izole edilmiştir. İzolatların moleküler karakterizasyonu York Üniversitesi Biyoloji Bölümü’nde yapılmıştır. Çalışma boyunca Prof. Dr. J. Peter W. Young ve Dr. Cyril Bontemps bilgi ve deneyimlerini paylaşarak destek olmuşlardır. Her iki araştırmacıya ve araştırmanın deneylerini gerçekleştirmiş olduğum kurumlara teşekkür ederim.

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